SUNDAY |
2:00 pm - 9:00 pm | Arrival and Check-in |
6:00 pm | Dinner |
7:30 pm - 9:30 pm | Cellular Osmoregulation: the interface of cell biology and physical biochemistry |
7:30 | Discussion Leader: Maurice Burg, National Institutes of Health, USA |
7:40 | M. Thomas Record, Jr., University of Wisconsin, Madison Escherichia coli as an osmotic system: changes in cytoplasmic amounts of water and solutes in response to osmotic stress and their predicted consequences for biopolymer processes |
8:10 | Discussion |
8:20 | Paul H. Yancey, Whitman College, USA
Lessons from nature: protecting but not overprotecting macromolecules with cellular osmolytes in habitat stresses from the Dead Sea to the deep sea |
8:50 | Discussion |
9:00 | Colloquium - Questions to be addressed during conference |
MONDAY |
7:30 am - 8:30 am | Breakfast |
8:30 am | Group Photo |
9:00 am - 12:30 pm | How do macromolecule-water-solute interactions modulate biochemical events? |
9:00 | Discussion Leaders: D. Wayne Bolen, University of Texas Medical Branch, USA & Paul H. Yancey, Whitman College, USA |
9:15 | Allen P Minton, National Institutes of Health, USA
Macromolecular excluded volume amplifies the dependence of intracellular reaction rates and equilibria upon cellular hydration |
9:45 | Discussion (Leader: D. Wayne Bolen) |
10:00 | Coffee |
10:40 | Kim D. Collins, University of Maryland Medical School, USA
The Role of Kosmotropes and Chaotropes in Cells |
11:10 | D. Wayne Bolen, University of Texas Medical Branch, USA
The Effects of Organic Osmolytes on the Kinetics of Folding and Unfolding |
11:40 | V. Adrian Parsegian, National Institutes of Health, USA Osmotic action on the nanometer scale |
12:10 | Discussion (Leader: Paul H. Yancy) |
12:30 pm | Lunch |
6:00 pm | Dinner |
7:30 pm - 9:30 pm | Transporters and channels that are osmosensors |
7:30 | Discussion Leader: Janet M Wood, University of Guelph, Canada |
7:35 | Reinhard Krämer, Universität Käln, Germany Osmosensing in the regulation of activity and transcription of BetP from Corynebacterium glutamicum |
8:05 | Discussion |
8:10 | Janos K Lanyi, University of
California, Irvine, USA Assembly of a hydrogen-bonded network of water accounts for osmotic effects in the transport cycle of
bacteriorhodopsin |
8:40 | Discussion |
8:45 | Boris Martinac, The University of Western Australia, Australia
Structure and function of mechanosensitive channels in prokaryotes |
9:15 | Discussion |
TUESDAY |
7:30 am - 8:30 am | Breakfast |
9:00 am - 12:30 pm | Solutes accumulation and its impact on membrane structure, stability and function |
9:00 | Discussion Leader: Stephen H. White, University of California at Irvine, USA |
9:10 | Michael Wortis, Simon Fraser University, Canada Tension and tension distribution in bilayer membranes. Newton's laws, continuum mechanics and molecular bonds |
9:40 | Discussion |
10:00 | Coffee |
10:40 | Bert Poolman, University of Groningen On the role of membrane lipids in osmosensing by an ABC transporter |
11:20 | Helena Santos, New University of Lisbon, Portugal
Biosynthesis of organic osmolytes from hyperthermophiles |
11:50 | John H. Crowe, University of California-Davis, USA
Lyotropic effects of water and sugars on biological membranes |
12:20 | Discussion |
12:30 pm | Lunch |
6:00 pm | Dinner |
7:30 pm - 9:30 pm | Transmembrane water and ion fluxes associated with osmoregulation |
7:30 | Discussion Leader: Wolfgang Epstein, University of Chicago, USA |
7:35 | Rainer Hedrich, Julius-von-Sachs-Institut fuer Biophysik, Germany
Regulation of the osmo-sensitive plant potassium channel GORK |
8:05 | Discussion |
| DEBATE |
8:10 | Thomas Zeuthen, University of
Copenhagen, Denmark Cotransporters as molecular water pumps - Are unstirred layer effects significant? |
8:30 | Jean-Yves Lapointe, University
of Montreal, Canada New evidence that the water flux associated with Na/glucose cotransport is essentially passive |
8:50 | Rebuttal - Thomas Zeuthen, University of
Copenhagen, Denmark |
9:00 | Rebuttal - Jean-Yves Lapointe, University
of Montreal, Canada |
9:10 | Discussion |
WEDNESDAY |
7:30 am - 8:30 am | Breakfast |
9:00 am - 12:30 pm | Channels implicated in osmoregulation |
9:00 | Discussion Leader: Ching Kung, University of Wisconsin-Madison, USA |
9:05 | Ian R. Booth, University of Aberdeen, Scotland Understanding mechanogating in bacteria - how MscS and MscL protect Escherichia coli against hypoosmotic stress |
9:35 | Andriy Anishkin, University of Maryland, USA
Reconstruction of the Gating Pathway of MscL In Situ: Spatial Scale of the Transition, Sequential Action of two Gates, Intermediate and Alternative Open States |
9:50 | Discussion |
10:00 | Coffee |
10:40 | Ralf Kaldenhoff, Universität
Würzburg, Germany Plant aquaporins: molecular function and physiological consequences for osmoregulated processes |
11:10 | Menachem Moshelion, Université catholique de Louvain, Belgium
Dynamic changes in osmotic water permeability of protoplast cell membrane during an osmotic challenge |
11:25 | Discussion |
11:35 | Eric Honoré, National Centre for Scientific Research, France The mechano-gated two P domain K+ channels |
12:05 | Discussion |
12:30 pm | Lunch |
6:00 pm | Dinner |
7:30 pm - 9:30 pm | Signal transduction and osmoregulation |
7:30 | Discussion Leader: Florian Lang, Universität Tübingen, Germany |
7:35 | Joan Ferraris, National Institutes of Health, USA
Tonicity-dependent transactivation of the mammalian Tonicity-Responsive Enhancer Binding Protein (TonEBP/OREBP) |
8:05 | Discussion |
8:10 | Else K Hoffmann, University of
Copenhagen, Denmark Intracellular signalling in cell volume regulation: Role in cell proliferation and apoptotic cell death |
8:40 | Discussion |
8:45 | Jian-Kang Zhu, University of Arizona, USA Signaling pathways mediating osmotic stress responses in Arabidopsis |
9:15 | Discussion |
THURSDAY |
7:30 am - 8:30 am | Breakfast |
9:00 am - 12:30 pm | Osmosensing in signal transduction |
9:00 | Discussion Leader: Karlheinz Altendorf, Universität Osnabrück, Germany |
9:05 | Jan Fassler, University of Iowa,
USA Osmotic Stress Sensing by the SLN1 Two Component Pathway in Yeast |
9:35 | Kay Marin, University of Rostock, Germany Identification of histidine kinases that act as sensors in the perception of salt stress in Synechocystis sp. PCC 6803 |
9:50 | Discussion |
10:00 | Coffee |
10:40 | Pauline Schaap, University of
Dundee, UK Anatomy of an adenylyl cyclase osmosensor of Dictyostelium discoideum |
11:10 | Discussion |
11:30 | Ching Kung, University of Wisconsin-Madison, USA The transient receptor potential channel on the yeast vacuole is mechanosensitive |
12:00 | Discussion |
12:30 pm | Lunch |
6:00 pm | Dinner |
7:30 pm - 9:30 pm | Structural remodelling of cells in response to osmotic stress |
7:30 | Discussion Leader: Stephan C. Schuster, Max-Planck-Institut für Entwicklungsbiologie, Tübingen, Germany |
7:35 | Rut Carballido-Lopez, University of Oxford, UK
Bacterial cytoskeleton: cell shape determination in Bacillus subtilis |
8:05 | Discussion |
8:10 | András Kapus, University Health Network &d University of Toronto, Canada Hyperosmotic stress induced remodeling of the cytoskeleton: role of Rho family small GTPases |
8:40 | Discussion |
8:45 | Angelika Noegel, University of Cologne,
Germany Cellular stress responses in Dictyostelium mutants |
9:15 | Discussion |
FRIDAY |
7:30 am - 8:30 am | Breakfast |
9:00 am | Depart |