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Three Dimensional Electron Microscopy
Gordon Research Conference

Breaking Barriers to Go Beyond Structure: Describing Functional States and Biological Context

Dates

June 21-26, 2015

Location

Colby-Sawyer College
New London, NH

Organizers

Chair:
Eva Nogales

Vice Chair:
Yifan Cheng

Meeting Description

The 2015 Gordon Research Conference on Three-Dimensional Electron Microscopy (3DEM) will continue its tradition of showcasing leading method development and biological research in three-dimensional structure determination, from macromolecules to cells.

Visualizing the structures of biological molecular machines is crucial for a mechanistic understanding of cellular function. 3DEM allows determination of structure and conformational dynamics of a wide variety of specimens, ranging from macromolecules and macromolecular assemblies to organelles and whole cells in their native environment. Progress in this field since this conference started in 1985 has been remarkable, and 3DEM has become a key method in both structural and cell biology. The technique has just entered a new era with the use of high-end electron microscopes coupled to new direct electron detection cameras that has led to high-resolution structural information using single particle cryo-EM, and unprecedented clarity in cellular ultrastructure characterization by electron tomography. Technical breakthroughs are pushing resolution limits, the breath of the applicability, and the biological insight provided by 3DEM. The conference will focus on the latest technological advances in instrumentation, software development, data processing, and tools for interpretation and validation that lead to improvements in quality, resolution, and reliability of the EM maps. It will also cover recent examples of how 3DEM is being used to elucidate biological function, with an emphasis on the description on multiple functional states and the biological context of macromolecules essential for their function.

The Conference will bring together the leading experts, junior researchers, and representatives of industrial partners in this exciting and rapidly evolving structural biology field. Following the tradition of previous conferences, the program will offer an attractive combination of lectures and poster sessions, with poster presenters selected for short talks during the course of the conference. The program will also provide excellent opportunities for discussions and informal gatherings. Due to limited attendance (<180 participants) and the over-subscribed nature of previous meetings, early registration is encouraged.

Contributors

Final Meeting Program

Sunday
2:00 pm - 9:00 pmArrival and Check-in
6:00 pmDinner
7:30 pm - 7:40 pmWelcome / Introductory Comments by GRC Site Staff
7:40 pm - 9:30 pmBiological Cutting Edge Methodologies Across Scales
Discussion Leader: Eva Nogales (University of California, Berkeley, USA)
7:40 pm - 7:50 pmOpening Remarks
7:50 pm - 8:25 pmDavid Baker (University of Washington, USA)
"Design of Novel Protein Structures, Functions, and Assemblies"
8:25 pm - 8:40 pmDiscussion
8:40 pm - 9:15 pmXiaowei Zhuang (Harvard University, USA)
"Super-Resolution Fluorescence Imaging with STORM"
9:15 pm - 9:30 pmDiscussion
Monday
7:30 am - 8:30 amBreakfast
9:00 am - 12:30 pmImproving Sample Preparation, Data Quality, Image Analysis and Resolution
Discussion Leader: Holger Stark (Max Planck Institute for Biophysical Chemistry, Germany)
9:00 am - 9:15 amIntroduction by Discussion Leader
9:15 am - 9:40 amNikolaus Grigorieff (Howard Hughes Medical Institute, USA)
"High-Dose Imaging for High-Resolution Cryo-EM"
9:40 am - 9:55 amDiscussion
9:55 am - 10:20 amHenning Stahlberg (University of Basel, Switzerland)
"High-Resolution Structures of Membrane Proteins by Cryo-EM of Small 2D Crystals of Limited Order"
10:20 am - 10:35 amDiscussion
10:35 am - 11:05 amCoffee Break
11:05 am - 11:30 amChristopher Russo (MRC Laboratory of Molecular Biology, United Kingdom)
"Applications of Ultrastable Gold Substrates in High Resolution Electron Cryomicroscopy and Electron Tomography"
11:30 am - 11:45 amDiscussion
11:45 am - 12:10 pmHong Zhou (University of California, Los Angeles, USA)
"Pushing the Limits for Cellular Machines with Volta Phase Plate, Imaging Filter and Electron Counting"
12:10 pm - 12:25 pmDiscussion
12:25 pm - 12:30 pmGeneral Discussion
12:30 pmLunch
1:30 pm - 4:00 pmFree Time
4:00 pm - 6:00 pmPoster Session
6:00 pmDinner
7:30 pm - 9:30 pmStructure in the Cellular Context
Discussion Leader: Dorit Hanein (Sanford Burnham Institute for Medical Research, USA)
7:30 pm - 7:35 pmIntroduction by Discussion Leader
7:35 pm - 8:00 pmWolfgang Baumeister (Max Planck Institute of Biochemistry, Germany)
"The Challenge of Doing Structural Biology In Situ"
8:00 pm - 8:15 pmDiscussion
8:15 pm - 8:40 pmDaniela Nicastro (Brandeis University, USA)
"Cryo-Electron Tomography and Tomography-Guided 3D Reconstruction of Cilia and Flagella"
8:40 pm - 8:55 pmDiscussion
8:55 pm - 9:15 pmJun LIu (University of Texas Medical School at Houston, USA)
"Visualizing Bacterial Type III Secretion Systems In Situ"
9:15 pm - 9:30 pmDiscussion
Tuesday
7:30 am - 8:30 amBreakfast
9:00 am - 12:30 pmInterpretation and Validation of Low-Medium Resolution Cryo-EM Structures
Discussion Leader: Hong-Wei Wang (Tsinghua University, China)
9:00 am - 9:15 amIntroduction by Discussion Leader
9:15 am - 9:40 amCarolyn Moores (ISMB, Birkbeck College London, United Kingdom)
"Conserved Mechanisms of the Microtubule-Regulated Kinesin ATPase"
9:40 am - 9:55 amDiscussion
9:55 am - 10:25 amGroup Photo / Coffee Break
10:25 am - 10:50 amGabriel Lander (The Scripps Research Institute, USA)
"Characterizing the Processive Dynein-Dynactin Complex"
10:50 am - 11:05 amDiscussion
11:05 am - 11:30 amJohn Rubinstein (Hospital for Sick Children / University of Toronto, Canada)
"Conformational Heterogeneity in Rotary ATPase"
11:30 am - 11:45 amDiscussion
11:45 am - 12:10 pmTanmay Bharat (MRC Laboratory of Molecular Biology, United Kingdom)
"Using Recent Advances in Single-Particle Cryo-EM Structure Determination for Sub-Tomogram Averaging"
12:10 pm - 12:25 pmDiscussion
12:25 pm - 12:30 pmGeneral Discussion
12:30 pmLunch
1:30 pm - 4:00 pmFree Time
4:00 pm - 6:00 pmPoster Session
6:00 pmDinner
7:30 pm - 9:30 pmSelected Poster Presentations: Recent Advances in 3D-EM
Discussion Leader: Edward Egelman (University of Virginia, USA)
7:30 pm - 7:40 pmMatteo Allegretti (Max Planck Institute of Biophysics, Germany)
"Structure of Polytomella ATP Synthase Dimers Give Insight into the Mechanism of Membrane Ion Translocation"
7:40 pm - 7:45 pmDiscussion
7:45 pm - 7:55 pmBenjamin Barad (University of California, San Francisco, USA)
"EMRinger: Side-Chain-Directed Model and Map Validation for 3D Electron Cryomicroscopy"
7:55 pm - 8:00 pmDiscussion
8:00 pm - 8:10 pmJose Maria Carazo Garcia (Spanish National Center for Biotechnolgy (CNB), Spain)
"AutoRes: Structure Resolution Determination Through Autocorrelation Analysis"
8:10 pm - 8:15 pmDiscussion
8:15 pm - 8:25 pmLeifu Chang (MRC Laboratory of Molecular Biology, United Kingdom)
"Structure and Mechanism of the Anaphase-Promoting Complex"
8:25 pm - 8:30 pmDiscussion
8:30 pm - 8:40 pmMichael Cianfrocco (Harvard University, USA)
"Low Cost, High Performance Processing of Single Particle Cryo-Electron Microscopy Data in the Cloud"
8:40 pm - 8:45 pmDiscussion
8:45 pm - 8:55 pmPaula Da Fonseca (MRC Laboratory of Molecular Biology, United Kingdom)
"Cryo-EM of Eukaryotic Inhibitor Bound 20S Proteasome Complexes as Tool to Develop New Therapeutic Drugs"
8:55 pm - 9:00 pmDiscussion
9:00 pm - 9:10 pmNatalya Dudkina (Birkbeck College, United Kingdom)
"Membrane Pore Formation in Pathogen Attack"
9:10 pm - 9:15 pmDiscussion
9:15 pm - 9:25 pmElizabeth Kellogg (University of California, Berkeley, USA)
"Stabilizing Mechanism of Microtubule-Targeting Agents at Near-Atomic Resolution Using Cryo-EM"
9:25 pm - 9:30 pmDiscussion
Wednesday
7:30 am - 8:30 amBreakfast
9:00 am - 12:30 pmStrategies for Atomic Modeling of High-Resolution Cryo-EM Structures
Discussion Leader: Steve Harrison (Harvard Medical School, USA)
9:00 am - 9:10 amIntroduction by Discussion Leader
9:10 am - 9:30 amAxel Brunger (Stanford University, USA)
"Refining Atomic Models with Non-Atomic Resolution"
9:30 am - 9:45 amDiscussion
9:45 am - 10:05 amSimon Jenni (Harvard Medical School, USA)
"Building a 2109 Residue Protein De Novo in a 3.9 A Resolution Map"
10:05 am - 10:20 amDiscussion
10:20 am - 10:45 amCoffee Break
10:45 am - 11:05 amPaul Adams (Lawrence Berkeley National Laboratory, USA)
"New Methods in Phenix for Real Space Refinement of Atomic Models Against Cryo-EM Maps"
11:05 am - 11:20 amDiscussion
11:20 am - 11:40 amFrank Dimaio (University of Washington, USA)
"Protein Structure Refinement at Near-Atomic Resolution with RosettaEM"
11:40 am - 11:55 amDiscussion
11:55 am - 12:15 pmAlan Brown (MRC Laboratory of Molecular Biology, United Kingdom)
"Coot and REFMAC for Model Building and Refinement into Cryo-EM Maps"
12:15 pm - 12:30 pmDiscussion
12:30 pmLunch
1:30 pm - 4:00 pmFree Time
4:00 pm - 6:00 pmPoster Session
6:00 pmDinner
7:00 pm - 7:30 pmBusiness Meeting
Nominations for the Next Vice Chair; Fill in Conference Evaluation Forms; Discuss Future Site and Scheduling Preferences; Election of the Next Vice Chair
7:30 pm - 9:30 pmStrategies to Analyze and Interpret Heterogeous Samples
Discussion Leader: Helen Saibil (Birkbeck College London, United Kingdom)
7:30 pm - 7:35 pmIntroduction by Discussion Leader
7:35 pm - 8:00 pmDmitry Lyumkis (The Salk Institute for Biological Studies, USA)
"Large-Scale 3D Heterogeneity Analysis Using Likelihood-Based Focused Classification in Frealign"
8:00 pm - 8:10 pmDiscussion
8:10 pm - 8:35 pmCharles Sindelar (Yale University, USA)
"New Approaches for Addressing Heterogeneity in Helical Filaments"
8:35 pm - 8:50 pmDiscussion
8:50 pm - 9:15 pmAbbas Ourmazd (University of Wisconsin - Milwaukee, USA)
"Mapping the Conformations and Energy Landscapes of Biological Machines"
9:15 pm - 9:30 pmDiscussion
Thursday
7:30 am - 8:30 amBreakfast
9:00 am - 12:30 pmCorrelative Methodologies and Large Scale Imaging
Discussion Leader: Grant Jensen (California Institute of Technology, USA)
9:00 am - 9:15 amIntroduction by Discussion Leader
9:15 am - 9:40 amPeijun Zhang (University of Pittsburgh, USA)
"Automation in Correlative Live Cell Light and Cryo-Electron Microscopy"
9:40 am - 9:55 amDiscussion
9:55 am - 10:20 amKay Gruenewald (University of Oxford, United Kingdom)
"Super-Resolution Cryo Microscopy Using Standard Fluorescent Protein"
10:20 am - 10:35 amDiscussion
10:35 am - 11:05 amCoffee Break
11:05 am - 11:30 amTao Xu (Chinese Academy of Sciences, China)
"Superresolution Correlative Imaging for Protein Localization in Cellular Context"
11:30 am - 11:45 amDiscussion
11:45 am - 12:10 pmBenjamin Kopek (Hope College, USA)
"Correlative Super-Resolution Fluorescence and Scanning Electron Microscopy"
12:10 pm - 12:25 pmDiscussion
12:25 pm - 12:30 pmGeneral Discussion
12:30 pmLunch
1:30 pm - 4:00 pmFree Time
4:00 pm - 6:00 pmPoster Session
6:00 pmDinner
7:30 pm - 9:30 pmSelected Poster Presentations: Recent Advances in the Application of Cryo-EM Methodology
Discussion Leader: Yifan Cheng (University of California, San Francisco, USA)
7:30 pm - 7:40 pmJacqueline Milne (National Institutes of Health, USA)
"Cryo-EM Structures of Metabolic Jacqueline Enzyme Complexes at Atomic Resolution"
7:40 pm - 7:45 pmDiscussion
7:45 pm - 7:55 pmAlexis Rohou (Janelia Research Campus, Howard Hughes Medical Institute, USA)
"Structure and Helical Variability of PhuZ Filaments in Different Nucleotide States"
7:55 pm - 8:00 pmDiscussion
8:00 pm - 8:10 pmCarsten Sachse (European Molecular Biology Laboratory (EMBL), Germany)
"Structural Basis of PB1 Domain-Mediated Polymerization by the p62/Nbr1 Autophagy Receptor"
8:10 pm - 8:15 pmDiscussion
8:15 pm - 8:25 pmFlorian Schur (European Molecular Biology Laboratory (EMBL), Germany)
"Resolving Retroviral Structures at Subnanometer Resolution Using Cryo-Electron Tomography and Subtomogram Averaging"
8:25 pm - 8:30 pmDiscussion
8:30 pm - 8:40 pmMichaela Spiegel (Forschungszentrum Jülich, ICS-6, Germany)
"Sorting Cryo-EM Images into Classes of Similar Molecular Conformations"
8:40 pm - 8:45 pmDiscussion
8:45 pm - 8:55 pmJanet Vonck (Max Planck Institute of Biophysics, Germany)
"Structure of Alcohol Oxidase by Cryo-EM"
8:55 pm - 9:00 pmDiscussion
9:00 pm - 9:10 pmElizabeth Wright (Emory University School of Medicine, USA)
"Correlative Microscopy Investigations of Paramyxovirus Assembly"
9:10 pm - 9:15 pmDiscussion
9:15 pm - 9:25 pmKai Zhang (MRC Laboratory of Molecular Biology, United Kingdom)
"The Structure of the Dynactin Complex and Its Interaction with Dynein"
9:25 pm - 9:30 pmDiscussion
Friday
7:30 am - 8:30 amBreakfast
9:00 amDeparture
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