| Sunday evening, July 5
|
Fast Breaking Developments |
|
Chair: Jeff Esko |
|
Bob Rosenberg (20') |
A new template for heparan sulfate biosynthesis |
|
Andrew Spicer (20') |
Eukaryotic hyaluronan synthases |
|
Paul Deangelis (5') |
An alternative reaction pathway for hyaluronan synthesis |
|
Israel Vlodavsky (20') |
Cloning, expression and function of mammalian heparanase |
|
Karen Dame (5') |
The degradation of heparan sulfate by heparanases is dependent on the distribution of sulfate/iduronate-rich domains |
|
Craig Freeman (5') |
Purification and characterization of mammalian heparanases |
| Monday morning, July 6
|
Proteoglycan Structure and Assembly |
|
Chairs: Koji Kimata and John Gallagher |
|
Hiroko Habuchi (20') |
Characterization and function of heparan sulfate
O-sulfotransferases |
|
Lena Kjellén (20') |
Targeted disruption of murine genes coding for heparan
sulfate glucosaminyl N-deacetylase/N-sulfotransferases |
|
Junichi Aikawa (5') |
Molecular cloning and tissue-specific expression of NDNS-3, a third member of the heparan sulfate N-deacetylase/N-sulfotransferase gene family |
|
Jaap van den Born (5') |
Differential inhibition of two glucosaminyl N-deacetylases/N-sulfotransferase isoforms by N-sulfated heparan sulfate/heparin domains revealed by a new ELISA |
|
Osami Habuchi (5') |
Purification and characterization of chondroitin 4-sulfotransferase from the culture medium of rat chondrosarcoma cells |
|
Hiroshi Kitagawa (5') |
Properties and differential gene expression of glucuronosyltransferase I involved in the biosynthesis of glycosaminoglycan-protein linkage region of proteoglycans |
|
Jeremy Turnbull (20') |
Strategies for direct sequencing of glycosaminoglycan
saccharides |
|
R. Sasiskeharan (20') |
Enzymatic degradation of heparin-like glycosaminoglycans |
|
Robert Kokenyesi (5') |
Regulation of dermatan/chondroitin sulfate size, sulfation and epimerization in a core protein specific manner |
| Monday Afternoon
|
Workshop: Genetic approaches for studying molecular interactions |
|
Chair: Andy Mendelsohn |
| Monday evening
|
Protein-Carbohydrate Interactions |
|
Chair: H. Edward Conrad |
|
Barbara Mulloy (20') |
Modeling heparin-protein interactions using the solution conformation of heparan as a 'molecular ruler' |
|
John Gallagher (20') |
Heparin sulfate: sequencing without end |
|
Andrew J. Day (20') |
The structure of hyaluronan binding domains |
|
James San Antonio (5') |
Defining the domains of Type I collagen involved in heparan binding and endothelial tube formation |
|
Markku Salmivirta (5') |
Growth factor recognition domains in heparan sulfate |
|
Narayanen Parthsarathy (5') |
Oligosaccharide sequence of heparan sulfate with high affinity for laminin |
| Tuesday morning, July 7
|
Signaling and Cytoskeletal Interactions |
|
Chair: John Couchman |
|
Guido David (20') |
Syntenin, a PDZ-protein that binds syndecan cytoplasmic domain |
|
Paul Goetinck (20') |
Signaling through syndecan-4 |
|
Alan Rapraeger (10') |
Tyrosine phosphorylation and binding interactions of syndecan cytoplasmic domains |
|
Cheryl Knudson (10') |
Hyaluronan binding to bovine articular chondrocytes alters CD44 phosphorylation and association with the cytoskeleton |
|
|
Cell Adhesion and Migration
|
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Chair: Markku Jalkanen |
|
Bryan Toole (20') |
Perturbation of hyaluronan interactions blocks tumor progression |
|
Lucille Wrenshall (5') |
Regulation by heparan sulfate and IL-1alpha of the ontogenic expression of TCR, CD4, and CD8 |
|
Anne Woods (20') |
Syndecans in adhesion and matrix assembly |
|
Markku Jalkanen (5') |
Activation of syndecan-1 enhancer (FiRE) in migrating cells of cutaneous wounds |
|
Joe Iida (5') |
Tyrosine phosphorylation of p130cas upon clustering of chondroitin sulfate proteoglycan in human melanoma cellsr |
| Tuesday evening
|
Microbial Adhesion and Model Organisms |
|
Chair: Magnus Höök |
|
X. Lin (20') |
Role of heparan sulfate proteoglycans in Wingless/Wnt and FGF signaling in Drosophila |
|
Michael Sarras (20') |
Cell/extracellular matrix interactions in Hydra as a model
epithelial system |
|
Carlos Semino (20') |
The Zebrafish embryo: A model for studying carbohydrate signaling molecules |
|
Xinhua Lin (20') |
Role of heparan sulfate proteoglycans in Wingless/Wnt and FGF signaling in Drosophila |
|
Andrew Spicer (10') |
Molecular cloning and characterization of human and mouse UDP-glucose dehydrogenase genes, the orthologues of Drosophila sugarless |
|
John Fessler (10') |
Papilin: Cloning and expression of an early mesoderm and basement membrane sulfated proteoglycan gene |
| Wednesday morning, July 8
|
Proteoglycans in Skeletal Development |
|
Chair: Anna Plaas |
|
Alan J. Grodzinsky (20') |
Cartilage matrix mechanics and remodeling in response to load |
|
Anders Apsberg (5') |
Fibulins are ligands for the selectin-like domains of aggrecan and versican |
|
Alan Murdoch (5') |
G3 domain and intracellular trafficking |
|
Marian Young (20') |
Mice with targeted disruption of the biglycan gene exhibit decreased bone density and increased expression of decorin |
|
Peter Roughley (5') |
Procollagen C proteinase and the conversion of prodecorin and probiglycan to their mature forms |
|
|
Proteoglycans in Neurobiology |
|
Chair: Arthur Lander |
|
Christine E. Holt (20') |
Heparan sulfates play a role in target recognition in the
developing visual system |
|
Sally Stringer (5') |
Role of the heparin-binding growth factors, glycosminoglycans and receptors in glial progenitor cell differentiation |
|
Yu Yamaguchi (20') |
Molecular interactions of lecticans and brain ECM |
|
Steve Carlson (5') |
The synaptic vesicle proteoglycan SV2 as a laminin receptor on nerve terminals |
| Wednesday evening
|
Proteoglycans in Cardiovascular Biology |
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Chair: Robert D. Rosenberg |
|
James A. Huntington (20') |
Structure of the core heparin pentasaccharide with antithrombin and the conformational change |
|
Ariadne de Agostini (5') |
Anticoagulant heparan sulfate proteoglycans in rat and human ovary |
|
Nick Shworak (5') |
Isolation, characterization, chromosomal localization and targeted disruption of the murine 30st1 gene |
|
Merton Bernfield (20') |
Syndecans in tissue repair: A delicate balance |
|
Roberto Perris (5') |
Expression mapping of PG-M/Versican isoforms in human tissues and structure/function studies of vascular isoforms |
|
John Whitelock (5') |
Adhesive properties of human perlecan for vascular cells |
|
Michael Simons (20') |
Ryudocan signaling in angiogenesis |
| Thursday morning, July 9
|
Gene Disruptions and Pathophysiology |
|
Chair: Renato Iozzo |
|
Lorne A. Clarke (20') |
Biochemical and pathologic features in a murine model for
MPS I: Insights into mucopolysaccharide storage diseases |
|
Shukti Chakravarti (20') |
Lumican, collagen fibril assembly and extracellular matrix architecture |
|
Jim Funderburgh (5') |
Biglycan stimulation of TGFß is correlated with development of cytoskeletal elements in cultured keratocytes |
|
Chia-Yang Liu (5') |
Molecular cloning and characterization of mouse keratocan, a cornea specific keratan sulfate proteoglycan |
|
Renato Iozzo (5') |
Antisense targeting of perlecan blocks tumor growth and angiogenesis in vivo |
|
Arthur Lander (20') |
Structure/function relationships in glypicans |
|
Jorge Filmus |
Role of glypican-3 in an overgrowth and dysmorphic syndrome |
|
Naoki Itano (5') |
Knockout mice of hyaluronan synthase-1 (HAS1) gene |
|
John McDonald (5') |
Molecular cloning and gene targeting of hyaluronan synthase-2 reveals a critical role in mouse cardiovascular development |
|
Barbara Vertel/Marvin Tanzer (5') |
Aggrecan domains and traffic control: direction and misdirection |
| Thursday evening
|
Therapeutic Strategies based on Proteoglycans and Glycosaminoglycans |
|
Chair: Merton Bernfield |
|
Ajit Varki |
Glycosaminoglycans as selectin ligands and therapeutic agents |
|
James Rusche |
Small molecule antagonists of GAG-protein interactions |
|
John J. Hopwood |
Replacement therapies for the treatment of mucopolysaccharidosis patients |