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Conference Program
 
Plant Senescence
June 27 - July 2, 2004
Mount Holyoke College
South Hadley, MA

Plant senescence is a highly regulated and complex process during which the plant reclaims as many mobilisable nutrients as possible from the senescing tissues. Genetic and molecular analyses suggest that the cell death associated with senescence is a form of programmed cell death (pcd). Situations of environmental abiotic and biotic stress can trigger senescence and/or other forms of pcd. Additionally, pcd is essential for certain aspects of plant development. This meeting will focus on how senescence and the various forms of plant pcd are regulated and executed.

Our goal is to bring together people using multi disciplinary approaches to the study of senescence and pcd. Gene isolation and molecular analysis have been the focus of much of the work in this area for the last decade but recent exponential advances in genomic analysis have revolutionised this approach. Additionally, the structure and dynamics of cellular and organismal function have begun to be analysed in the context of senescence and pcd. A new session on systems biology will investigate the use of these approaches. Knowledge of genes is only a beginning; we need to have a clear understanding of where and how those gene products act within the cell. The meeting will integrate genetic and molecular approaches with other disciplines such as cell biology, biochemistry and physiology. A systematic analysis of recycling processes and investigation of the role of organelles in senescence and cell death may well reveal exciting links between the different death processes.

From a practical viewpoint, expanding our knowledge of the processes of plant senescence has many potential economic benefits. The timing and efficiency of senescence has an important role in determining the yield and pre-harvest quality of many cereal, forage and horticultural crops. In addition, a significant proportion of all fresh plant products for food or feed are lost, or suffer from reduced quality, due to plant senescence. For example, environmental stress leading to premature senescence can result in loss of yield and reduced nutritional quality. Post harvest senescence and consequent loss of nutrients are important economic problems causing substantial losses to producers as well as loss of quality and nutritional value to the consumer. Therefore increased knowledge of the regulation and processes of senescence will provide information for plant breeders to generate crops with improved yield, stress-resistance and also post-harvest quality with benefits for growers, suppliers and consumers.


Contributors
We would like to thank our contributors for their generous support of this meeting: the National Science Foundation, the University of Warwick, the University of Chicago, the Gatsby Foundation, Syngenta, the American Floral Endowment, Advanced Technologies Cambridge, Monsanto and Pioneer-Hybred International.

SUNDAY
2:00 pm - 9:00 pmArrival and Check-in
6:00 pmDinner
7:30 pm - 9:30 pmKEY NOTE SESSION: OVERVIEWS OF LIFESPAN, CELL DEATH AND CELL FATE CONTROL
Discussion Leader: Vicky Buchanan-Wollaston (HRI Wellesbourne)
7:30 pm - 7:45 pmWelcome Remarks
7:45 pm - 8:10 pmEric Baehrecke (University of Maryland)
"Genetic regulation of programmed cell death during Drosophila development"
8:20 pm - 8:45 pmGordon Lithgow (Buck Institute, CA)
"Stress response as the determinant of organismal senescence - the lifespan of C. elegans"
8:55 pm - 9:20 pmArp Schnittger (Max Planck Inst, Koeln)
"Arabidopsis trichomes as model cells to study the connection between cell fate, cell cycle and cell death"
MONDAY
7:30 am - 8:30 amBreakfast
8:45 amGroup Photo
9:00 am - 12:30 pmGENETIC CONTROL OF SENESCENCE, RIPENING AND CELL DEATH
Discussion Leader: Harry Klee (University of Florida)
9:00 am - 9:20 amHong Gil Nam (Pohang University of Science and Technology)
"Involvement of cytokinin signaling components in control of leaf senescence in Arabidopsis"
9:30 am - 9:50 amDominique Roby (INRA/CNRS)
"Functions and targets of AtMYB30, a transcriptional regulator of the hypersensitive cell death in Arabidopsis"
10:00 amCoffee Break
10:30 am - 10:50 amPaul Dijkwel (University of Groningen)
"Arabidopsis genes that regulate ethylene-induced senescence"
11:00 am - 11:20 amGuri Johal (Purdue University)
"Identifying natural suppressors of cell death and senescence in plants: implications in breeding"
11:30 am - 11:50 am James Giavannoni (Cornell University)
"Positional cloning of ripening mutations impacting fruit ripening and senescence in tomato"
12:00 pm - 12:20 pmGary Drews (University of Utah)
"Cell death during female gametophyte development"
12:30 pmLunch
1:30 pm - 4:00 pmFree Time
4:00 pm - 6:00 pmPoster Session
6:00 pmDinner
7:30 pm - 9:30 pmCELL DEATH AND CELL SEPARATION IN ORGAN DEVELOPMENT
Discussion Leader: Tony Bleecker (University of Wisconsin, Madison)
7:30 pm - 7:50 pmSara Liljegren (University of North Carolina)
"Cell separation in Arabidopsis flowers and fruit"
8:00 pm - 8:20 pmJerry Roberts (University of Nottingham)
"Molecular and genetic strategies to dissect the abscission process in Arabidopsis"
8:30 pm - 8:50 pmHilary Rogers (Cardiff University)
"Gene expression during Alstroemeria floral senescence"
9:00 pm - 9:20 pmHiroo Fukuda (University of Tokyo)
"Formation and rupture of a lytic vacuole is a pivotal step of programmed cell death in plants"
TUESDAY
7:30 am - 8:30 amBreakfast
9:00 am - 12:30 pmSIGNALING IN CELL DEATH RESPONSES
Discussion Leader: Dominique Roby (INRA/CNRS)
9:00 am - 9:20 amJeff Dangl (University of North Carolina)
"Control of cell death by the LSD1 gene familty"
9:30 am - 9:50 amImre Somssich (Max-Planck-Institut, Koeln)
"WRKY transcription factors involved in cross-talk between defense and senescence signaling pathways"
10:00 amCoffee Break
10:30 am - 10:50 amHarry Klee (University of Florida)
"Control of ethylene responses at the level of receptor function"
11:00 am - 11:20 amSusheng Gan (Cornell University)
"Functional analysis of leaf senescence-associated transcription factors in Arabidopsis"
11:30 am - 11:50 amLiz Harrison (HRI Wellesbourne)
"Unravelling the signalling pathways that control gene expression during senescence"
12:00 pm - 12:20 pmCarmen Castresana (Universidad Autónoma)
"Role of oxylipins as cell death protectants"
12:30 pmLunch
1:30 pm - 4:00 pmFree Time
4:00 pm - 6:00 pmPoster Session
6:00 pmDinner
7:30 pm - 9:30 pmBIOCHEMISTRY AND PHYSIOLOGY OF SENESCENCE AND CELL DEATH
Discussion Leader: Howard Thomas (Institute of Grassland and Environmental Research)
7:30 pm - 7:50 pmPaul Bethke (University of California, Berkeley)
"Active oxygen and nitric oxide as determinants of programmed death in barley aleurone cells"
8:00 pm - 8:20 pmKlaus Apel (ETH, Swiss Federal Institute of Technology)
"Singlet oxygen and the role of a genetically defined cell death program in Arabidopsis thaliana"
8:30 pm - 8:50 pmStefan Hörtensteiner (Institute of Plant Sciences, BERN)
"Biochemical and molecular analysis of chlorophyll catabolism"
9:00 pm - 9:20 pmSergi Munné-Bosch (Universitat de Barcelona)
"The role of chloroplastic antioxidants in the ageing process of Mediterranean shrubs"
WEDNESDAY
7:30 am - 8:30 amBreakfast
9:00 am - 12:30 pmMECHANICS OF CELLULAR RECYCLING DURING SENESCENCE AND CELL DEATH
Discussion Leader: Russell Jones (University of California, Berkeley)
9:00 am - 9:20 amDave Robinson (University of Heidelberg)
"Autophagy: what (plant) cells do when they are hungry"
9:30 am - 9:50 amIkuko Hara-Nishimura (Kyoto University)
"A vacuolar processing enzyme exhibiting caspase-1-like activity regulates TMV-induced cell death in tobacco leaves"
10:00 amCoffee Break
10:30 am - 10:50 amJan Smalle (University of Wisconsin, Madison)
"Functions of the Ubiquitin/26S proteasome pathway in Arabidopsis hormone signaling and senescence"
11:00 am - 11:20 amJohn Thompson (University of Waterloo)
"Regulation of programmed cell death by eucaryotic translation initiation factor 5a: Implications for growth and development of plants"
11:30 am - 11:50 amHelen Ougham (IGER)
"Chloroplast senescence: there and back again"
12:00 pm - 12:30 pmRyouichi Tanaka (Hokkaido University)
"Light-dependent and -independent cell death caused by accumulation of pheophorbide a in transgenic Arabidopsis plants expressing antisense RNA for pheophorbide a oxygenase"
12:30 pmLunch
1:30 pm - 4:00 pmFree Time
4:00 pm - 6:00 pmPoster Session
6:00 pmDinner
7:30 pm - 7:50 pmBusiness meeting
7:50 pm - 9:30 pmHOT TOPICS
Discussion Leader: Jean T. Greenberg (University of Chicago)
Dingzhong Tang (Indiana University)
"Control of pathogen-induced cell death by EDR1 and EDR2"
Elin Johansson (Göteborg University)
"Progression and Specificity of Protein Oxidation in the Life Cycle of Arabidopsis thaliana"
Maria Suarez (University of Agricultural Science, Uppsala SWEDEN)
"Metacaspase-dependent programmed cell death is essential for plant embryogenesis"
Lirong Zeng (Ohio State University)
"Understanding the molecular mechanisms of a novel U-box/ARM-repeat protein-regulated programmed cell death and broad-spectrum disease resistance in rice"
Andreas M. Fischer (Montana State University)
"Functional analysis of cereal leaf senescence using a genetic approach"
Kohki Yoshimoto (National Institute for Basic Biology)
"Plant Autophagy: Autophagy-Defective Mutants Exhibit Early Senescence"
THURSDAY
7:30 am - 8:30 amBreakfast
9:00 am - 12:30 pmORGANELLE AND CYTOSKELETON FUNCTIONS DURING SENESCENCE AND CELL DEATH
Discussion Leader: Hiroo Fukuda (University of Tokyo)
9:00 am - 9:20 amGreg C Vanlerberghe (University of Toronto)
"The mitochondrial respiratory chain and programmed cell death in plants"
9:30 am - 9:50 amNoni Franklin-Tong (University of Birmingham)
"Self-incompatibility triggers programmed cell death in Papaver pollen"
10:00 amCoffee Break
10:30 am - 10:50 amChristine Gietl (Technische Universitaet Muenchen)
"Ricinosomes: an organelle for developmentally regulated programmed cell death in senescing plant tissue"
11:00 am - 11:20 amNan Yao (University of Chicago)
"Cell biological analysis of mechanisms of programmed cell death in Arabidopsis protoplasts"
11:30 am - 11:50 amPeter V. Bozhkov (University of Agricultural Science, Uppsala SWEDEN)
"Cell-death programme of plant embryo"
12:00 pm - 12:20 pmRenier Van Der Hoorn (Wageningen University)
"Activity profiling of papain-like proteases in plants"
12:30 pmLunch
1:30 pm - 4:00 pmFree Time
4:00 pm - 6:00 pmPoster Session
6:00 pmDinner
7:30 pm - 9:30 pmSYSTEMS BIOLOGY AS A MEANS TO UNDERSTAND SENESCENCE AND CELL DEATH
Discussion Leader: Guri Johal (Purdue Univeristy)
7:30 pm - 7:50 pmNick Smirnoff (University of Exeter)
"Role of ascorbate and other antioxidants in senescence and cell death"
8:00 pm - 8:20 pmShimon Gepstein (Technion University)
"Proteomics profiling of leaf senescence"
8:30 pm - 8:50 pmJörg Kudla (Universitat Münster)
"Stress, Calcium and protein phosphorylation: The role of CBL calcium sensor/CIPK protein kinase signaling network"
9:00 pm - 9:20 pmAllan Shapiro (U. of Delaware)
"A Systems Biology Approach to Control over Programmed Cell Death in the Arabidopsis Hypersensitive Response"
FRIDAY
7:30 am - 8:30 amBreakfast
9:00 amDepart

 
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