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Three Dimensional Electron Microscopy
Visualizing Biological Processes with 3DEM
June 24-29, 2007
Colby-Sawyer College
New London, NH
Chair:
Phoebe L. Stewart

Vice Chair:
Werner Kuehlbrandt

The theme of the 2007 Three Dimensional Electron Microscopy GRC meeting is Visualizing Biological Processes with 3D EM. Three dimensional structural information can be obtained for large macromolecules and macromolecular assemblies such as the ribosome or icosahedral viruses via single particle reconstruction; for biological samples arrayed in two-dimensional crystals via electron crystallography; for helical filaments via helical reconstruction; or for molecular or cellular samples via electron tomography. This conference will provide a forum for the latest biological results, as well as for technological developments including EM instrumentation and image processing software. Additional speakers will be selected at the meeting for the Poster Presentations and Discussion sessions from among the poster presenters. Future conferences include a June 15-20, 2008 meeting to be held at Il Ciocco, Barga, Italy chaired by Werner Kuehlbrandt; a 2009 meeting to be held in the United States chaired by Stephen Fuller; and a 2010 meeting to be held in Europe chaired by Holger Stark.

The Chair-Elect for 2009 and Poster Chair for this year's meeting is Stephen Fuller. The Social Chair is Andy Hoenger.

Contributors
Gordon Research Conferences
FEI Company
Agouron Institute
National Institutes of Health
Gatan, Inc.
JEOL USA, Inc.
Novartis Vaccines and Diagnostics, Inc.
Protochips, Inc.
Image Science Software GMBH
Tietz Video and Image Processing Systems GMBH
E.A. Fischione Instruments, Inc.
Cell Press
SUNDAY
2:00 pm - 11:00 pmArrival and Check-in
6:00 pmDinner
7:30 pm - 7:40 pmWelcome / Introductory Comments by GRC Site Staff
7:40 pm - 9:30 pmVISUALIZING BIOLOGICAL PROCESSES WITH 3D EM
7:40 pm - 7:50 pmDiscussion Leader: Phoebe Stewart (Vanderbilt University Medical Center)
Introduction and Overview
7:50 pm - 8:25 pmTony Crowther (MRC Laboratory of Molecular Biology)
"Cryomicroscopy of Viruses: Variations on a Theme"
8:25 pm - 8:40 pmDiscussion
8:40 pm - 9:15 pmRon Milligan (The Scripps Research Institute)
"Visualizing Mechanochemical Mechanisms in the Kinesins"
9:15 pm - 9:30 pmDiscussion
MONDAY
7:30 am - 8:30 amBreakfast
9:00 am - 12:30 pmRNA AND RIBOSOME TRANSACTIONS
9:00 am - 9:10 amDiscussion Leader: Michael Radermacher (University of Vermont)
Introduction and Overview
9:10 am - 9:35 amJean-Francois Menetret (Boston University School of Medicine)
"Ribosome Binding of a Single Copy of the Secy/Sec61 Complex: Implications for the Initiation of Protein Translocation"
9:35 am - 9:50 amDiscussion
9:50 am - 10:15 amJan Giesebrecht (Charité - Universitätsmedizin Berlin)
"Analysis of Functional 80S Intermediates of the Mammalian Ribosomal Elongation Cycle by Multi-Particle Cryo-EM"
10:15 am - 10:30 amDiscussion
10:30 amCoffee Break / Group Photo
11:10 am - 11:35 amGregory Allen (HHMI / Wadsworth Center)
"Cryo-EM of a Chimeric Ribosome-Group II Intron Complex: a General Method for Imaging Large, Structured RNAs"
11:35 am - 11:50 amDiscussion
11:50 am - 12:15 pmB.V.V. Prasad (Baylor College of Medicine)
"Structural Insights into Rotavirus Replication"
12:15 pm - 12:30 pmDiscussion
12:30 pmLunch
1:30 pm - 4:00 pmFree Time
4:00 pm - 6:00 pmPoster Session
6:00 pmDinner
7:30 pm - 9:30 pmDNA TRANSACTIONS
7:30 pm - 7:40 pmDiscussion Leader: Niko Grigorieff (HHMI / Brandeis University)
Introduction and Overview
7:40 pm - 8:05 pmFrancisco Asturias (The Scripps Research Institute)
"Structure of a RSC-Nucleosome Complex and Implications for the Chromatin Remodeling Mechanism"
8:05 pm - 8:15 pmDiscussion
8:15 pm - 8:40 pmArnaud Amzallag (Ecole Polytechnique Fédérale de Lausanne)
"3D Reconstruction and Analysis of Shapes of DNA Minicircles Observed by Cryo-Electron Microscopy"
8:40 pm - 8:50 pmDiscussion
8:50 pm - 9:15 pmTakayuki Kato (Osaka University)
"Structural Analysis of DNA Tetrahedron"
9:15 pm - 9:30 pmDiscussion
TUESDAY
7:30 am - 8:30 amBreakfast
9:00 am - 12:30 pmISOLATED PROTEINS AND PROTEIN FILAMENTS
9:00 am - 9:10 amDiscussion Leader: Daniela Nicastro (Brandeis University)
Introduction and Overview
9:10 am - 9:35 amDeborah Kelly (Harvard Medical School)
"Monolayer Purification: A Rapid Method for Isolating Protein Complexes for Single Particle EM"
9:35 am - 9:50 amDiscussion
9:50 am - 10:15 amCharles Sindelar (Lawrence Berkeley National Laboratory)
"Nanometer-Resolution Studies of Human Conventional Kinesin Complexed to the Microtubule"
10:15 am - 10:30 amDiscussion
10:30 amCoffee Break
11:10 am - 11:35 amGrant Jensen (California Institute of Technology)
"Electron Cryotomography of the Bacterial Cytoskeleton"
11:35 am - 11:50 amDiscussion
11:50 am - 12:15 pmDorit Hanein (The Burnham Institute)
"Three-Dimensional Structure of the Arp2/3 Branch Junction"
12:15 pm - 12:30 pmDiscussion
12:30 pmLunch
1:30 pm - 4:00 pmFree Time
4:00 pm - 6:00 pmPoster Session
6:00 pmDinner
7:30 pm - 9:30 pmPOSTER PRESENTATIONS AND DISCUSSION I
Discussion Leader: Ingeborg Schmidt-Krey (Georgia Institute of Technology)
TBA (8 speakers, 10 min presentation and 5 min discussion each)
WEDNESDAY
7:30 am - 8:30 amBreakfast
9:00 am - 12:30 pmHIGHER ORDER ASSEMBLIES
9:00 am - 9:10 amDiscussion Leader: James Conway (University of Pittsburgh School of Medicine)
Introduction and Overview
9:10 am - 9:35 amBeate Rockel (Max Planck Institute of Biochemistry)
"Electron Microscopy of a Giant Protease"
9:35 am - 9:50 amDiscussion
9:50 am - 10:15 amJack Johnson (The Scripps Research Institute)
"Late Stage HK97 Virus Particle Maturation: Visualizing a Brownian Ratchet with Crystallography and CryoEM"
10:15 am - 10:30 amDiscussion
10:30 amCoffee Break
11:10 am - 11:35 amThomas Marlovits (IMP-IMBA Research Center, Austrian Academy of Sciences)
"Reprogramming the Type III Injectisome"
11:35 am - 11:50 amDiscussion
11:50 am - 12:15 pmSteven Ludtke (Baylor College of Medicine)
"Backbone Trace and Dynamics of GroEL by Single Particle Reconstruction"
12:15 pm - 12:30 pmDiscussion
12:30 pmLunch
1:30 pm - 4:00 pmFree Time
4:00 pm - 6:00 pmPoster Session
6:00 pmDinner
7:00 pm - 7:30 pmBusiness Meeting
(Nominations and election for the Chair of the 2011 meeting in the US, who will also be the Poster Chair for the 2009 meeting in the US; Fill out Conference Evaluation Forms; Discuss future Site & Scheduling preferences)
7:30 pm - 9:30 pmMEMBRANE PROTEINS AND MEMBRANE PROXIMAL EVENTS
7:30 pm - 7:40 pmDiscussion Leader: Werner Kuehlbrandt (Max Planck Institute of Biophysics)
Introduction and Overview
7:40 pm - 8:05 pmBernard Heymann (National Institute of Arthritis and Musculoskeletal and Skin Diseases)
"Cryo-Electron Tomography of Coated Vesicles: Distribution of Particle Forms and Defining their Components"
8:05 pm - 8:15 pmDiscussion
8:15 pm - 8:40 pmMark Yeager (The Scripps Research Institute)
"Structure of Full-length HIV-1 CA by Electron Cryo-Crystallography: A Model for the Mature Capsid Lattice"
8:40 pm - 8:50 pmDiscussion
8:50 pm - 9:15 pmStephen Fuller (Wellcome Trust Centre for Human Genetics)
"Cryo-Electron Microscopy Provides Insights into how HIV Cheats at Assembly and Infection"
9:15 pm - 9:30 pmDiscussion
THURSDAY
7:30 am - 8:30 amBreakfast
9:00 am - 12:30 pmTECHNICAL ADVANCES
9:00 am - 9:10 amDiscussion Leader: Bridget Carragher (The Scripps Research Institute)
Introduction and Overview
9:10 am - 9:35 amJens Meiler (Vanderbilt University)
"EM-Fold: A Novel Approach for Protein Structure Elucidation from Cryo-EM Density Maps"
9:35 am - 9:50 amDiscussion
9:50 am - 10:15 amRadostin Danev (Okazaki Institute for Integrative Bioscience)
"Single Particle Analysis Based on Zernike Phase Contrast TEM"
10:15 am - 10:30 amDiscussion
10:30 amCoffee Break
11:10 am - 11:35 amHarald Rose (Technical University Darmstadt)
"Criteria and Prospects for Realizing Optimum Electron Microscopes"
11:35 am - 11:50 amDiscussion
11:50 am - 12:15 pmSriram Subramaniam (National Cancer Institute)
"3D Imaging of Mammalian Cells with Ion-Abrasion Scanning Electron Microscopy"
12:15 pm - 12:30 pmDiscussion
12:30 pmLunch
1:30 pm - 4:00 pmFree Time
4:00 pm - 6:00 pmPoster Session
6:00 pmDinner
7:30 pm - 9:30 pmPOSTER PRESENTATIONS AND DISCUSSION II
Discussion Leader: Esther Bullitt (Boston University School of Medicine)
TBA (8 speakers, 10 min presentation and 5 min discussion each)
FRIDAY
7:30 am - 8:30 amBreakfast
9:00 amDepart
 
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