Conference Program
 
Diffraction Methods In Structural Biology
July 13-18, 2008
Bates College
Lewiston, ME
Chair:
Elspeth F. Garman

Vice Chair:
Andrew G.W. Leslie

Structural Biology is a field which underpins our basic understanding of the molecular basis of disease, and hence of disease prevention. Diffraction of X-rays from crystalline samples is used to obtain accurate information on the three-dimensional structure of large biologically important molecules: this knowledge often allows the mechanisms of action of molecular pathways to be determined, and provides the potential to interfere with them if they have undesirable outcomes. The continuing development of experimental and computational methods is crucial to the successful application of the technique to increasingly complex biological systems.

This GRC seeks to cover the latest advances in all aspects of Diffraction Methods for Structural Biology. Sessions are planned on the experimental and computational aspects, future directions in synchrotron based macromolecular crystallography, challenging problems, pushing the boundaries of the technique, emerging technologies, complementary methods and imaging methods of the future.

Contributors
AMGEN, INC.
AREA DETECTOR SYSTEMS CORPORATION
BLAKE INDUSTRIES, INC.
BRISTOL-MYERS SQUIBB COMPANY
BRUKER AXS (ANALYTICAL X-RAY SYSTEMS)
CCP4
CELL PRESS
GENENTECH, INC.
GILEAD SCIENCES
HAMPTON RESEARCH CORPORATION
MARRESEARCH GMBH
MERCK & CO., INC.
MOLECULAR DIMENSIONS LIMITED
MONSANTO COMPANY
NATIONAL INSTITUTES OF HEALTH
RAYONIX L.L.C.
RIGAKU AMERICAS CORPORATION
WYETH RESEARCH
SUNDAY
2:00 pm - 11:00 pmArrival and Check-in
6:00 pmDinner
7:30 pm - 7:35 pmWelcome / Introductory Comments by GRC Site Staff
7:35 pm - 9:30 pmMACROMOLECULAR STRUCTURES: PUSHING THE BOUNDARIES
7:35 pm - 7:40 pm Discussion Leader: Janet Smith (University of Michigan at Ann Arbor, USA)
7:40 pm - 8:25 pm Jens Preben Morth (Aarhus University, Denmark)
"Structure determination of the sodium-potassium pump"
8:25 pm - 8:35 pm Discussion
8:35 pm - 9:20 pm Jack Johnson (Scripps Research Institute, USA)
"Crystallography of Viral Maturation Intermediates: Large Unit Cells, Particle Dynamics, and Energy Landscapes"
9:20 pm - 9:30 pm Discussion
MONDAY
7:30 am - 8:30 amBreakfast
9:00 am - 12:30 pmEXPERIMENTAL ASPECTS
9:00 am - 9:05 am Discussion Leader: James Holton (University of California San Fancisco and Lawrence Berkeley National Laboratory, USA)
9:05 am - 9:40 am Douglas Juers (Whitman College, USA)
"Rational approaches to cryo-crystallography protocols"
9:40 am - 9:50 am Discussion
9:50 am - 10:15 am Coffee Break / Group Photo
10:15 am - 10:50 am Zbyszek Dauter (Frederick Cancer Institute, USA)
"A thoughtful approach to data collection"
10:50 am - 11:00 am Discussion
11:00 am - 11:35 am Eddie Snell (Hauptman-Woodward Medical Research Institute, USA)
"Are X-rays damaging to structural biology? A case study with xylose isomerase"
11:35 am - 11:45 am Discussion
11:45 am - 12:20 pm Martin Weik (Institut de Biologie Structurale, Grenoble, France)
"Temperature-controlled cryo-crystallography to study the structural dynamics of proteins"
12:20 pm - 12:30 pm Discussion
12:30 pmLunch
1:30 pm - 4:00 pmFree Time
4:00 pm - 6:00 pmPoster Session
6:00 pmDinner
7:30 pm - 9:30 pmCOMPLEMENTARY TECHNIQUES
7:30 pm - 7:35 pm Discussion Leader: Mitchell Guss (University of Sydney, Australia)
7:35 pm - 8:05 pm Junko Yano (Lawrence Berkeley National Laboratory, USA)
"Combination of XAS and XRD for studying a high-resolution structure of the photosynthetic water-splitting catalyst"
8:05 pm - 8:15 pm Discussion
8:15 pm - 8:45 pm Dean Myles (Oak Ridge National Laboratory USA)
"New opportunities for neutron structural biology"
8:45 pm - 8:55 pm Discussion
8:55 pm - 9:25 pm Arwen Pearson (University of Leeds, UK)
"Single crystal spectroscopy coupled to crystallography"
9:25 pm - 9:30 pm Discussion
TUESDAY
7:30 am - 8:30 amBreakfast
9:00 am - 12:30 pmFUTURE DIRECTIONS IN SYNCHROTRON BASED MACROMOLECULAR CRYSTALLOGRAPHY
9:00 am - 9:05 am Discussion Leader: Elizabeth Duke (Diamond Light Source, UK)
9:05 am - 9:40 am Soichi Wakatsuki (KEK, Japan)
"Microfocus and low energy PX developments looking towards next generation synchrotron sources"
9:40 am - 9:50 am Discussion
9:50 am - 10:25 am Ed Mitchell (ESRF, Grenoble, France)
"Bigger, better, faster, more: The ESRF Upgrade Programme"
10:25 am - 10:35 am Discussion
10:35 am - 11:00 am Coffee Break
11:00 am - 11:35 am Aina Cohen (SSRL, USA)
"Automation, Robotics and Remote Access at the SSRL Protein Crystallography Beam Lines"
11:35 am - 11:45 am Discussion
11:45 am - 12:20 pm Frank von Delft (Structural Genomics Consortium, Oxford, UK)
"A User's Wish: An Experiment-Focussed Beamline Interface"
12:20 pm - 12:30 pm Discussion
12:30 pmLunch
1:30 pm - 4:00 pmFree Time
4:00 pm - 6:00 pmPoster Session
6:00 pmDinner
7:30 pm - 9:30 pmEMERGING TECHNOLOGIES
7:30 pm - 7:35 pm Discussion Leader: Andrew Leslie (MRC-LMB, Cambridge, UK)
7:35 pm - 8:05 pm Robert F. Fischetti (GM/CA-CAT, Argonne National Laboratory, USA)
"Where have all the photoelectrons gone?"
8:05 pm - 8:15 pm Discussion
8:15 pm - 8:45 pm David Flot (EMBL-Grenoble, France)
"What can we expect from a small beam in protein crystallography? The example of ID23-2, at the ESRF"
8:45 pm - 8:55 pm Discussion
8:55 pm - 9:25 pm Clemens Schulze-Briese (Swiss Light Source, Villigen, Switzerland)
"PILATUS 6M - the first year of regular user operation"
9:25 pm - 9:30 pm Discussion
WEDNESDAY
7:30 am - 8:30 amBreakfast
9:00 am - 12:30 pmCOMPUTATIONAL METHODS
9:00 am - 9:05 am Discussion Leader: Airlie McCoy (University of Cambridge, UK)
9:05 am - 9:40 am Sasha Popov (ESRF, Grenoble, France)
"BEST SAD data collection"
9:40 am - 9:50 am Discussion
9:50 am - 10:25 am Paul Adams (Lawrence Berkeley Laboratory, USA)
"Automated structure solution with PHENIX"
10:25 am - 10:35 am Discussion
10:35 am - 11:00 am Coffee Break
11:00 am - 11:35 am Kevin Cowtan (University of York, UK)
"The 3 R's of automated model building: R-factors, resolution, and refinement"
11:35 am - 11:45 am Discussion
11:45 am - 12:20 pm George Phillips (University of Wisconsin-Madison, USA)
"Interpretation of electron density maps from protein crystals"
12:20 pm - 12:30 pm Discussion
12:30 pmLunch
1:30 pm - 4:00 pmFree Time
4:00 pm - 6:00 pmPoster Session
6:00 pmDinner
7:00 pm - 7:30 pmBusiness Meeting
(Nominations for the next Vice Chair; Fill out Conference Evaluation Forms; Discuss future Site & Scheduling preferences; Election of the next Vice Chair)
7:30 pm - 9:30 pmCHALLENGING PROBLEMS / MEMBRANE PROTEINS
7:30 pm - 7:35 pm Discussion Leader: Tom Terwilliger (Los Alamos National Laboratory, USA)
7:35 pm - 8:05 pm Chris Tate (LMB-MRC Cambridge, UK)
"Thermostabilisation and structure determination of a β1 adrenergic receptor"
8:05 pm - 8:15 pm Discussion
8:15 pm - 8:45 pm Mike Lawrence (The Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia)
"Pursuit of the structure of the human insulin receptor ectodomain"
8:45 pm - 8:55 pm Discussion
8:55 pm - 9:25 pm Mark Mayer (NIH, Bethesda, USA)
"Structure and function of allosteric ion binding sites in glutamate receptor ligand binding domains"
9:25 pm - 9:30 pm Discussion
THURSDAY
7:30 am - 8:30 amBreakfast
9:00 am - 10:30 amTALKS SELECTED FROM POSTERS
9:00 am - 9:05 am Discussion Leader: Ana Gonzales (Stanford Synchrotron Radiation Laboratory, USA)
9:05 am - 9:20 am Talk to be selected from posters
9:20 am - 9:25 am Discussion
9:25 am - 9:40 am Talk to be selected from posters
9:40 am - 9:45 am Discussion
9:45 am - 10:00 am Talk to be selected from posters
10:00 am - 10:05 am Discussion
10:05 am - 10:20 am Talk to be selected from posters
10:20 am - 10:25 am Discussion
10:25 am - 10:50 am Coffee Break
10:50 am - 12:30 pmVALIDATION
Discussion Leader: Ana Gonzales (Stanford Synchrotron Radiation Laboratory, USA)
10:50 am - 11:00 amAnastassis Perrakis (Netherlands Cancer Institute, The Netherlands)
"An initiative for better validation tools to aid the peer review process"
11:00 am - 11:35 am Gerard Kleywegt (University of Uppsala, Sweden)
"Validation"
11:35 am - 11:45 am Discussion
11:45 am - 12:20 pm Jane Richardson (Duke University, USA)
"MolProbity Progress: RNA, Auto-corrections, and H redux"
12:20 pm - 12:30 pm Discussion
12:30 pmLunch
1:30 pm - 4:00 pmFree Time
4:00 pm - 6:00 pmPoster Session
6:00 pmDinner
7:30 pm - 9:30 pmIMAGING METHODS OF THE FUTURE
7:30 pm - 7:35 pm Discussion Leader: Raimond Ravelli (Leiden University Medical Centre, The Netherlands)
7:35 pm - 8:20 pm Steve Ludtke (Baylor College of Medicine, Houston, USA)
"Resolution and Molecular Motion, Frontiers in CryoEM"
8:20 pm - 8:30 pm Discussion
8:30 pm - 9:15 pm Carolyn Larabell (Lawrence Berkeley National Laboratory)
"Putting molecules in context with soft x-ray tomography"
9:15 pm - 9:25 pm Discussion
FRIDAY
7:30 am - 8:30 amBreakfast
9:00 amDepart
Funding for this conference was made possible (in part) by 1 R13 GM084483 from NIGMS. The views expressed in written conference materials or publications and by speakers and moderators do not necessarily reflect the official policies of the Department of Health and Human Services; nor does not mention trade names, commercial practices, or organizations imply endorsement by the U.S. Government.