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Conference Program
 
Plant Cell Walls
Plant Cell Wall Biosynthesis
August 2-7, 2009
Bryant University
Smithfield, RI
Chair:
Debra Mohnen

Vice Chair:
Jocelyn K. Rose

Related Meeting Information
The Plant Cell Walls Gordon Research Conference was held in conjunction with the Plant Cell Walls Gordon-Kenan Research Seminar. Please refer to the Plant Cell Walls GKRS web page for more information.

Plant cell walls are a complex cellular compartment essential for plant growth, development and response to biotic and abiotic stress and a major biological resource for meeting our future bioenergy and natural product needs. The goal of the 2009 Plant Cell Walls Gordon Research Conference is to summarize and critically evaluate the current level of understanding of the structure, synthesis and function of the whole plant extracellular matrix, including the polysaccharides, proteins, lignin and waxes that comprise the wall, and the enzymes and regulatory proteins that drive wall synthesis and modification. Innovative techniques to study how both primary and secondary wall polymers are formed and modified throughout plant growth will be emphasized, including rapid advances taking place in the use of anti-wall antibodies and carbohydrate binding proteins, comparative and evolutionary wall genomics, and the use of mutants and natural variants to understand and identify wall structure-function relationships. Discussions of essential research advances needed to push the field forward toward a systems biology approach will be highlighted. The meeting will include a commemorative lecture in honor of the career and accomplishments of the late Emeritus Professor Bruce A. Stone, a pioneer in wall research who contributed over 40 years of outstanding studies on plant cell wall structure, function, synthesis and remodeling including emphasis on plant cell wall beta-glucans and arabinogalactans.

The dwindling supply of fossil fuels will not suffice to meet our future energy and industrial product needs. Plant biomass is the renewable resource that will fill a large part of the void left by vanishing fossil fuels. It is therefore critical that basic research scientists interact closely with industrial researchers to critically evaluate the current state of knowledge regarding how plant biomass, which is largely plant cell walls, is synthesized and utilized by the plant. A final goal of the meeting is to bring together academic, research center, and industrial scientists to identify the most crucial and fundamental basic research questions and directions that will supply the information needed to understand, modify and use plant biomass for human industrial and energy needs.


Contributors
National Science Foundation (NSF)
Megazyme International Ireland Limited
Pioneer Hi-Bred International, Inc.
Syngenta
Carl Storm Underrpresented Minority Fellowships
Carl Storm International Diversity Fellowship
Gordon Research Conferences

SUNDAY
2:00 pm - 9:00 pmArrival and Check-in (Office Closed 6:00 pm - 7:00 pm)
6:00 pmDinner
7:30 pm - 7:40 pmWelcome / Introductory Comments by GRC Site Staff
7:40 pm - 7:45 pmWelcome / Introductory Comments by GRC Conference Chairs
7:45 pm - 9:30 pm Keynote Lectures: Understanding Plant Cell Wall Structure and Function - the Challenge to Meet Future Biofuel and Natural Product Needs
Discussion Leader: Kenneth Keegstra (Michigan State University, USA)
7:50 pm - 8:30 pmAlan Darvill (University of Georgia (UGA)
"Plant Cell Wall Polysaccharide Structures"
8:30 pm - 8:40 pm Discussion
8:40 pm - 9:20 pm Jing-Ke Weng (Purdue University)
"An ancient divergence in plant lignin biosynthetic strategies"
9:20 pm - 9:30 pm Discussion
MONDAY
7:30 am - 8:30 amBreakfast
8:30 amGroup Photo
9:00 am - 12:30 pm The Entire Plant Cell Wall: Polysaccharides, Proteins, Lignin and Waxes
Discussion Leaders: Paul Knox (University of Leeds) and Paul Dupree (University of Cambridge)
9:00 am - 9:20 am Jocelyn Rose (Cornell)
"Developing a toolbox to probe the mysterious cuticular cell wall"
9:20 am - 9:30 am Discussion
9:30 am - 9:50 amJohn Ralph (University of Wisconsin)
"High-resolution Solution-state NMR of Unfractionated Plant Cell Walls"
9:50 am - 10:00 am Discussion
10:00 am Coffee Break
10:30 am - 10:50 am Michael Hahn (UGA)
"Generation and characterization of new antibodies against pectin and xyloglucan epitopes"
10:50 am - 11:00 am Discussion
11:00 am - 11:20 am Tatyana Gorshkova (Kazan Institute of Biochemistry and Biophysics, Russia)
"What are pectins doing in the secondary cell wall of plant fibers?"
11:20 am - 11:30 am Discussion
11:30 am - 11:40 am Candace Haigler (North Carolina State University)
"Cell wall dynamics mediating cotton fiber differentiation"
11:40 am - 11:45 am Discussion
11:45 am - 11:55 am Li Tan (Ohio University)
"Structures of Hyp-arabinogalactans from AGP fusion proteins and wild type AGPs"
11:55 am - 12:00 pm Discussion
12:00 pm - 12:30 pm General Discussion
12:30 pmLunch
1:30 pm - 4:00 pmFree Time
4:00 pm - 6:00 pmPoster Session I
6:00 pmDinner
7:30 pm - 9:30 pm Evolution and Wall Structural Diversity
Discussion Leaders: Malcolm O’Neill (UGA) and Jim Wade (Gevo, Inc)
7:30 pm - 7:50 pm Zoë Popper (National University of Galway, Ireland)
"The diversity of plant cell walls"
7:50 pm - 8:00 pm Discussion
8:00 pm - 8:20 pmWilliam Willats (University of Copenhagen, Denmark)
"Diversity and evolution of plant cell walls"
8:20 pm - 8:30 pm Discussion
8:30 pm - 8:50 pm Stephen Fry (University of Edinburgh, UK)
"Polysaccharides that undergo transglycosylation in the cell walls of diverse land plants and charophytic algae"
8:50 pm - 9:00 pm Discussion
9:00 pm - 9:10 pmJose Esteves (University of Buenos Aires)
"Discovery of lignin in seaweed reveals convergent evolution of cell-wall architecture"
9:10 pm - 9:15 pm Discussion
9:15 pm - 9:30 pm General Discussion
TUESDAY
7:30 am - 8:30 amBreakfast
9:00 am - 12:30 pm Wall Biosynthesis: Emerging Paradigms
Discussion Leaders: Kanwarpal Dhugga (Dupont) and Henrik Scheller (Lawrence Berkeley National Lab)
9:00 am - 9:20 am Maor Bar-Peled (UGA)
"Sir, Can I use AcetylCoA?"
9:20 am - 9:30 am Discussion
9:30 am - 9:50 am Vincent Bulone (Royal Institute of Technology, Sweden)
"Understanding cellulose biosynthesis: challenges, update and perspectives"
9:50 am - 10:00 am Discussion
10:00 am Coffee Break
10:30 am - 10:50 am Seth Debolt (University of Kentucky)
"Chemical genetic dissection of plant cell walls"
10:50 am - 11:00 am Discussion
11:00 am - 11:20 am William York (UGA)
"Integrating biochemical, structural and genetic analyses of xylan biosynthesis"
11:20 am - 11:30 am Discussion
11:30 am - 11:40 am Jesper Harholt (University of Copenhagen)
"Analyzing whole genomes with regard to CAZy-family entries: A phylogenetic study of Physcomitrella, Selaginella and Brachypodium"
11:40 am - 11:45 am Discussion
11:45 am - 12:05 pm Taku Demura (Nara Inst. Sci. Tech./RIKEN Plant Science Center, Japan)
"New Components Participating in the Secondary Cell Wall Formation"
12:05 pm - 12:15 pm Discussion
12:15 pm - 12:30 pm General Discussion
12:30 pmLunch
1:30 pm - 4:00 pmFree Time
4:00 pm - 6:00 pmPoster Session II
6:00 pmDinner
7:30 pm - 9:30 pm Systems Biology of Cell Walls - What Do We Need to Get There?
Discussion Leaders: Harry Brumer (Royal Institute of Technology, Sweden) and William York (UGA)
7:30 pm - 7:50 pmBernard Henrissat (CNRS, France)
"Systems Biology of Cell Walls. What do we need to get there? A CAZy perspective"
7:50 pm - 8:00 pm Discussion
8:00 pm - 8:20 pm Bruce Link (Syngenta)
"Systems Biology, Agricultural Crops, and the Cell Wall"
8:20 pm - 8:30 pm Discussion
8:30 pm - 8:40 pmRowan Mitchell (Rothamsted Research)
"The wheat endosperm cell wall as a model system for studying grass xylans"
8:40 pm - 8:45 pm Discussion
8:45 pm - 8:55 pmMarcin Nowicki (Michigan State University)
"Plant Nucleotide-Sugar Metabolism: Evidence for Substrate Channeling in Pectin Biosynthesis"
8:55 pm - 9:00 pm Discussion
9:00 pm - 9:10 pmTony Arioli (Bayer Cropscience)
"Molecular characterization of a novel cell wall strength mechanism in high quality cotton fiber"
9:10 pm - 9:15 pm Discussion
9:15 pm - 9:30 pm General Discussion
WEDNESDAY
7:30 am - 8:30 amBreakfast
9:00 am - 12:30 pm Plant Cell Walls: Cell Biology and Plant Development
Discussion Leaders: Bruce Link (Syngenta Biotechnology, Inc.) and Alison Roberts (University of Rhode Island)
9:00 am - 9:20 amSimon Turner (University of Manchester, UK)
"Assembly and trafficking of the cellulose synthase complex in developing xylem"
9:20 am - 9:30 am Discussion
9:30 am - 9:40 amCharles Anderson (UC Berkeley)
"Real Time Visualization of Cellulose Reorientation in Expanding Arabidopsis Cell Walls"
9:40 am - 9:45 am Discussion
9:45 am - 9:55 amLutz Neumetzler (Max-Planck-Institute of Molecular Plant)
"Golgi localized V-ATPase activity is necessary for trafficking of the cellulose synthase complex"
9:55 am - 10:00 am Discussion
10:00 am Coffee Break
10:30 am - 10:50 amNorman Lewis (Washington State University)
"Lignin Configuration: An Assumption of Convenience"
10:50 am - 11:00 am Discussion
11:00 am - 11:10 amChang-Jun Liu (Brookhaven National Laboratory)
"Understanding Plant Cell Wall Acylesterification and Its Biological Function"
11:10 am - 11:15 am Discussion
11:15 am - 11:25 amElizabeth Crowell (INRA Versailles)
"Pausing of Golgi bodies on Microtubules Regulates Secretion of Cellulose Synthase Complexes in Arabidopsis"
11:25 am - 11:30 am Discussion
11:30 am - 11:40 amKevin Vaughn (ARS-USDA-SWSRU)
"Ballistic Seed Dispersal in Bittercress is due to Juxtaposition of Mucilage and Highly Thickened Cells"
11:40 am - 11:45 am Discussion
11:45 am - 11:55 amArun Sampathkumar (Max Planck Institute of Molecular Plant Physiology)
"Role of actin cytoskeleton during primary wall cellulose production"
11:55 am - 12:00 pm Discussion
12:00 pm - 12:30 pm General Discussion
12:30 pmLunch
1:30 pm - 4:00 pmFree Time
4:00 pm - 6:00 pmPoster Session III
6:00 pmDinner
7:00 pm - 7:30 pmBusiness Meeting
(Nominations for the next Vice Chair; Fill out Conference Evaluation Forms; Discuss future Site & Scheduling preferences; Election of the next Vice Chair)
7:30 pm - 9:30 pm Keynote Lectures: Plant Cell Walls in Grasses and Probing Wall Synthesis - Perspective in Honor of Bruce Stone
Discussion Leader: Mary Tierney (University of Vermont)
7:30 pm - 7:50 pmHerman Hofte (INRA)
"The role of cell wall metabolism in plant development"
7:50 pm - 8:00 pm Discussion
8:00 pm - 8:20 pm Geoff Fincher (University of Adelaide, Australia)
"Biosynthesis of cell wall polysaccharides in the grasses: determinants of fine structure"
8:20 pm - 8:30 pm Discussion
8:30 pm - 8:50 pm Tony Bacic (University of Melbourne, Australia)
"Cell wall biosynthesis and the cell wall - plasma membrane continuum"
8:50 pm - 9:00 pm Discussion
9:00 pm - 9:15 pm General Discussion
9:15 pm - 9:30 pm Tribute to Bruce Stone: Friend, colleague, mentor
THURSDAY
7:30 am - 8:30 amBreakfast
9:00 am - 11:30 am 3D Structure and Function of Wall Modifying Enzymes
Discussion Leaders: Barry McCleary (Megazyme) and Bernard Henrissat (CNRS, France)
9:00 am - 9:20 am Harry Brumer (Royal Institute of Technology, Sweden)
"Fold diversity in plant polysaccharide degradation"
9:20 am - 9:30 am Discussion
9:30 am - 9:50 am Mats Ohlin (Lund University, Sweden)
"Evolution for function - tailoring of carbohydrate-specific probes for use in plant research"
9:50 am - 10:00 am Discussion
10:00 am Coffee Break
10:30 am - 10:50 am Daniel Cosgrove (Penn State University)
"Structure-based analysis of expansin functions"
10:50 am - 11:00 am Discussion
11:00 am - 11:10 am Cecile Herve (University of Leeds)
"Differential recognition of plant cell walls by glycoside hydrolases and cell wall probes"
11:10 am - 11:15 am Discussion
11:15 am - 11:30 am General Discussion
11:30 am - 12:30 pm Walls as Biomass for Biofuels and Bioproducts
Discussion Leaders: Will Rottmann (ArborGen) and Markus Pauly (Michigan State University)
11:30 am - 11:50 amKenneth Keegstra (MSU, GRBRC)
"Understanding hemicellulose biosynthesis and manipulating hemicellulose levels in plants"
11:50 am - 12:00 pm Discussion
12:00 pm - 12:10 pmTomoyuki Konishi (Forestry and Forest Products Research Institute)
"Down-regulating UDP-arabinopyranose mutase reduces the arabinose content of rice cell walls and increases the walls susceptibility to enzymatic saccharification"
12:10 pm - 12:15 pm Discussion
12:15 pm - 12:30 pm General Discussion
12:30 pmLunch
1:30 pm - 4:00 pmFree Time
4:00 pm - 6:00 pmPoster Session IV
6:00 pmDinner
7:30 pm - 9:00 pm Walls as Biomass for Biofuels and Bioproducts, cont.
Discussion Leaders: Maud Hinchee (ArborGen) and Markus Pauly (Michigan State University)
7:30 pm - 7:50 pmHenrik Scheller (LBNL, JBEI)
"Identification of targets for modification of hemicellulose composition"
7:50 pm - 8:00 pm Discussion
8:00 pm - 8:10 pmAngela Ziebell (NREL)
"Chemical extraction of down-regulated C3H and HCT Alfalfa reveals structural differences in lignin"
8:10 pm - 8:15 pm Discussion
8:15 pm - 8:25 pmPrajakta Pradhan (Joint Bioenergy Insitute - LBNL)
"Synthesis of phenylpropanoid-esters and -amides in Arabidopsis thaliana to engineer a cleavable lignin"
8:25 pm - 8:30 Discussion
8:30 pm - 8:50 pmRichard Dixon (Noble Foundation, BESC)
"Lignin modification- the good, the bad, and the ugly"
8:50 pm - 9:00 pm Discussion
FRIDAY
7:30 am - 8:30 amBreakfast
9:00 amDeparture

 
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