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Three Dimensional Electron Microscopy
Gordon Research Conference

Technical Advances and Recent Breakthroughs in 3DEM

Dates

June 28 - July 3, 2009

Location

Colby-Sawyer College
New London, NH

Organizers

Chairs:
Andreas Hoenger & Stephen D. Fuller

Vice Chair:
Holger Stark

Meeting Description

The GRC on 3-D Electron microscopy has recently doubled it’s meeting frequency from being held every two years in the US to an annual event alternating between the US and Europe. Despite this increase in meeting capacity the recent conferences were still oversubscribed and hopeful applicants had to be deterred. This development clearly demonstrates the strong interest in 3DEM methods and applications for biological specimens, and that the conference serves a growing community. In particular, since the rediscovery of (cryo-) electron tomography for large cellular structures the demand for 3DEM has literally exploded despite a current limitation in resolution to approx. 2nm due to technical limitations. On the other end, recent work has shown that electron microscopy data of biological structures may reach atomic resolution beyond 0.2nm. In-between these two extremes hybrid methods, combining intermediate resolution EM 3D maps with X-ray, NMR or even in-silico data have been improved tremendously and generated a lot of excitement for thorough investigations into protein structure and function relationships. Obviously these developments are as usual accompanied and made possible by new products from the relevant industries ranging for microscope technology (LHe-lenses, corrector lenses, energy filtering, phase-plates improved stages) to detector design (lens-coupled CCD systems, CMOS detectors etc.). At the same time data acquisition and image analysis software products are constantly improved and refined, playing their crucial role on the data handling and interpretation front.

The GRC conference on 3DEM has always been one of the most exciting meetings in the field and it brought together the leaders in electron microscopy with the next generation microscopists as well as industrial partners. The format allows for ample discussion and exchange between the official presentations, which is an extremely successful concept that has nothing lost of its attraction. As on previous conferences the program will include formal presentations and poster presentations. Due to limited attendance (max. 150 participants) early registration is recommended.

Meeting Program

SUNDAY
2:00 pm - 9:00 pmArrival and Check-in (Office Closed 6:00 pm - 7:00 pm)
6:00 pmDinner
7:30 pm - 7:40 pmWelcome / Introductory Comments by GRC Site Staff
7:40 pm - 9:30 pmKeynote Lectures
Discussion Leaders: Stephen Fuller (Univ. of Oxford Welcome Trust, Oxford U.K.) and Andreas Hoenger (Univ. of Colorado, Boulder CO)
7:40 pm - 8:20 pm J. Richard McIntosh (Univ. of Colorado, Boulder CO)
"3D EM of the Mitotic Spindle: is evolution and prospects"
8:20 pm - 8:35 pm Discussion
8:35 pm - 9:15 pm Andreas Engel (Univ. of Basel, Switzerland and Case Western Univ., Cleveland OH)
"Reaching the Resolution limits in 3D-EM"
9:15 pm - 9:30 pm Discussion
MONDAY
7:30 am - 8:30 amBreakfast
9:00 am - 12:30 pmExciting News from the Single-Particle & Viral Community
Discussion Leader: Trevor Sewell (Univ. of Capetown, ZA)
9:05 am - 9:30 am Elena Orlova (Birbeck College, London U.K.)
"Heterogeneity analysis of macromolecular systems by electron microscopy"
9:30 am - 9:40 am Discussion
9:40 am - 10:05 am Christian Spahn (Charite, Berlin Germany)
"Conformational modes of ribosomal complexes"
10:05 am - 10:15 am Discussion
10:15 am - 10:45 am Coffee Break / Group Photo
10:45 am - 11:10 am Joachim Frank (Columbia Univ., New York NY)
"Cognate vs. near-cognate aminoacyl-tRNA discrimination on the ribosome"
11:10 am - 11:20 am Discussion
11:20 am - 11:45 am James Chen (Brandeis Univ., Waltham MA)
"Single-Particle EM Structural Analysis of the Rotavirus Virion at Near-Atomic Resolution"
11:45 am - 11:55 am Discussion
11:55 am - 12:20 pm Hong Zhou (UCLA, Los Angeles CA)
"Building atomic models for large dsRNA viruses by single particle cryoEM"
12:20 pm - 12:30 pm Discussion
12:30 pmLunch
1:30 pm - 4:00 pmFree Time
4:00 pm - 6:00 pmPoster Session
6:00 pmDinner
7:30 pm - 9:30 pmCryo-Tomography and 3D volume averaging on macromolecular structures
Discussion Leader: Achilleas Frangakis (EMBL-Heidelberg, Germany)
7:30 pm - 7:50 pm John Briggs (EMBL-Heidelberg, Germany)
"Understanding Retrovirus structure using subtomogram averaging"
7:50 pm - 8:00 pm Discussion
8:00 pm - 8:20 pm Julia Zanetti (Univ. of Oxford Welcome Trust, Oxford U.K.)
"Contrast Transfer Function correction applied to cryo-electron tomography and sub-tomogram averaging"
8:20 pm - 8:30 pm Discussion
8:30 pm - 8:50 pm Julia Cope (Univ. of Colorado, Boulder CO)
"Structural insights into the Unusual Kinesin Kar3Vik1"
8:50 pm - 9:00 pm Discussion
9:00 pm - 9:20 pm Kfir Ben-Harush (Ben Gurion Univ., Negev Israel)
"The Supramolecular Organization of Nuclear Lamins"
9:20 pm - 9:30 pm Discussion
TUESDAY
7:30 am - 8:30 amBreakfast
9:00 am - 12:30 pmExciting News from the 2-D Crystal / Helical Community
Discussion Leader: Jenny Hinshaw (NIH-NIDDK, Bethesda MD)
9:05 am - 9:30 am Ed Egelman (Univ. of Virginia, Charlottesville VA)
"Polymorphism and Heterogeneity in Helical Polymers: Implications for Evolutionary Divergence"
9:30 am - 9:40 am Discussion
9:40 am - 10:05 am Charles Sindelar (Brandeis Univ., Waltham MA)
"A unified microtubule-driven nucleotide response pathway for the kinesin motors"
10:05 am - 10:15 am Discussion
10:15 am - 10:40 am Jason Mears (NIH-NIDDK, Bethesda MD)
"The structure of Dnm1, the yeast dynamin involved in mitochondria fission, using single particle helical methods"
10:40 am - 10:50 am Discussion
10:50 am - 11:10 am Coffee Break
11:10 am - 11:35 am Matthias Wolf (Harvard Medical School, MA)
"Bovine Papillomavirus at Near-Atomic Resolution"
11:35 am - 11:45 am Discussion
11:45 am - 12:10 pm Vincenz Unger (Yale Univ., New Haven CT)
"Membrane Scaffolds - The Next Frontier"
12:10 pm - 12:20 pm Discussion
12:30 pmLunch
1:30 pm - 4:00 pmFree Time
4:00 pm - 6:00 pmPoster Session
6:00 pmDinner
7:30 pm - 9:30 pmSelected Poster Presentations
Discussion Leader: Holger Stark (MPI Goettingen, Germany)
(speakers will be identified for short talks at the conference from the poster sessions)
WEDNESDAY
7:30 am - 8:30 amBreakfast
9:00 am - 12:30 pmExciting News from the Hybrid Community (new data & software)
Discussion Leader: Dorit Hanein (Burnham Inst., La Jolla CA)
9:05 am - 9:30 am Dorit Hanein (Burnham Inst., La Jolla CA)
"The Structural Organization of the Actin Cytoskeleton at the Lamella, a correlative LM-EM study"
9:30 am - 9:40 am Discussion
9:40 am - 10:05 am Bing Jap (UC-Berkeley, Berkeley CA)
"Hybrid Structure of Tripeptidyl Peptidase II"
10:05 am - 10:15 am Discussion
10:15 am - 10:40 am Giovanni Cardone (NIH-NIAMS, Bethesda MD)
"Molecular architecture of retrovirus capsids: pentamers and hexamers of the two-domain CA protein"
10:40 am - 10:50 am Discussion
10:50 am - 11:10 am Coffee Break
11:10 am - 11:35 am Alex Rigort (MPI Martinsried, Germany)
"Hybrid Approaches: Navigating and Micromachining Cellular Samples for Cryo-Electron Tomography"
11:35 am - 11:45 am Discussion
11:45 am - 12:10 pm Keiichi Namba (Osaka University, Japan)
"New insights into the structure and function of bacterial flagella at near-atomic detail"
12:10 pm - 12:20 pm Discussion
12:30 pmLunch
1:30 pm - 4:00 pmFree Time
4:00 pm - 6:00 pmPoster Session
6:00 pmDinner
7:00 pm - 7:30 pmBusiness Meeting
(Nominations for the next Vice Chair; Fill out Conference Evaluation Forms; Discuss future Site & Scheduling preferences; Election of the next Vice Chair)
7:30 pm - 9:30 pmFancy Accessories to EMs: Phase plates, Correctors, Image Cameras
Discussion Leader: Robert Glaeser (UC-Berkeley, Berkeley CA)
7:35 pm - 8:00 pm Radostin Danev (Okazaki Institute for Integrative Bioscience, Japan)
"Zernike Phase Contrast Cryotomography"
8:00 pm - 8:10 pm Discussion
8:10 pm - 8:35 pm Max Haider (CEOS GmbH, Heidelberg Germany)
"Can inelastically scattered electrons contribute constructively to image formation?"
8:35 pm - 8:45 pm Discussion
8:45 pm - 9:30 pm Panel Discussion: "Properties of CMOS detectors"
Peter Denes (UC-Berkeley) and Frank De Jonge (FEI Company, Eindhoven, NL)
THURSDAY
7:30 am - 8:30 amBreakfast
9:00 am - 12:30 pmCryo-Tomography of large cellular structures and intact small cells
Discussion Leader: Grant Jensen (CalTech, Pasadena CA)
9:05 am - 9:30 am Kay Grunewald (MPI Martinsried, Germany)
"In situ analysis of macromolecular virus-host-interactions"
9:30 am - 9:40 am Discussion
9:40 am - 10:05 am Martin Pilhofer (CalTech, Pasadena CA)
"Bacterial Tubulin: a Novel Member of the Bacterial Cytoskeleton"
10:05 am - 10:15 am Discussion
10:15 am - 10:40 am Pamela Bjorkman (CalTech, Pasadena CA)
"Antibody transport across epithelial cells revealed by electron tomography"
10:40 am - 10:50 am Discussion
10:50 am - 11:10 am Coffee Break
11:10 am - 11:35 am Jason Lanman (The Scripps Res. Inst., La Jolla CA)
"Visualizing Flock House Virus assembly in Drosophila cells with electron microscope tomography"
11:35 am - 11:45 am Discussion
11:45 am - 12:10 pm Sriram Subramaniam (NIH, Bethesda MD)
"3D Imaging of HIV Neutralization, Entry and Transport"
12:10 pm - 12:20 pm Discussion
12:30 pmLunch
1:30 pm - 4:00 pmFree Time
4:00 pm - 6:00 pmPoster Session
6:00 pmDinner
7:30 pm - 9:30 pmSelected Poster Presentations
Discussion Leader: Holger Stark (MPI, Goettingen Germany)
(speakers will be identified for short talks at the conference from the poster sessions)
FRIDAY
7:30 am - 8:30 amBreakfast
9:00 amDepart

Funding for this conference was made possible (in part) by 5 R13 GM085826-01 from National Institute of General Medical Sciences. The views expressed in written conference materials or publications and by speakers and moderators do not necessarily reflect the official policies of the Department of Health and Human Services; nor does mention by trade names, commercial practices, or organizations imply endorsement by the U.S. Government.
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