Gordon Research Conferences
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Conference Program
 
Protein Cofactors, Radicals and Quinones
January 24-29, 2010
Four Points Sheraton / Holiday Inn Express
Ventura, CA

The Gordon Research Conference on Protein Cofactors, Radicals and Quinones was initiated in 1990 and has since been held bi-annually. The conference will focus on the remarkable ways to generate and utilize sophisticated cofactors that enzymes have evolved. Our knowledge in this fascinating field is continuously extending in novel directions. This conference will focus on radical-containing cofactors, assembly of complex metal cofactors, metal cofactors involved in radical reactions, cofactors derived from covalently cross-linked amino acid residues, and quinone cofactors derived from aromatic side chains. This rapidly developing field with ample medical implications brings together scientists from a variety of disciplines in chemistry and biology, including mechanistic enzymology, structural biology, metallobiochemistry, bioorganic chemistry, molecular biology, spectroscopy and computational biochemistry.

In addition to talks by invited speakers, the 2010 Gordon Research Conference on Protein Cofactors, Radicals and Quinones will also select several poster presenters for short talks based on the poster abstract. We especially encourage assistant professors, postdoctoral associates and graduate students to apply to the conference and submit poster abstracts. The poster sessions are important integral parts of the conference, and provide exceptional opportunity for young scientists to present and informally discuss their recent results. We look forward to meet you all in January!


Contributors

SUNDAY
4:00 pm - 8:00 pmArrival and Check-in (Office Closed 6:00 pm - 7:45 pm)
6:00 pmDinner
7:30 pm - 7:40 pmWelcome / Introductory Comments by GRC Site Staff
7:40 pm - 9:30 pmELECTRON TRANSFER IN PROTEINS
Discussion Leader: Judith P. Klinman (University of California Berkeley, CA, USA)
7:40 pm - 8:20 pm Harry B. Gray (Caltech, Pasadena, CA, USA)
"Electron flow through proteins"
8:20 pm - 8:35 pm Discussion
8:35 pm - 9:15 pm Daniel G. Nocera (MIT, Cambridge, MA, USA)
"The road that radicals pave to the large scale deployment of solar energy"
9:15 pm - 9:30 pm Discussion
MONDAY
7:30 am - 8:30 amBreakfast
8:30 amGroup Photo
9:00 am - 12:30 pmRIBONUCLEOTIDE REDUCTASES
Discussion Leader: J. Martin Bollinger, Jr. (Penn State University, University Park, PA, USA)
9:00 am - 9:35 am JoAnne Stubbe (MIT, Cambridge, MA, USA)
"Unnatural amino acids as probes of PCET: RNR as a paradigm"
9:35 am - 9:45 am Discussion
9:45 am - 10:20 am David R. Edgell (University of Western Ontario, London, ON, Canada)
"Assembly of a split ribonucleotide reductase by unique interaction domains"
10:20 am - 10:30 am Discussion
10:30 am Coffee Break
11:00 am - 11:35 am Astrid Gräslund (Stockholm University, Sweden)
"The iron-manganese cofactor in chlamydial ribonucleotide reductase and related proteins"
11:35 am - 11:45 am Discussion
11:45 am - 12:20 pm Per E.M. Siegbahn (Stockholm University, Sweden)
"Theoretical studies on ribonucleotide reductase"
12:20 pm - 12:30 pm Discussion
12:30 pmLunch
1:30 pm - 4:00 pmFree Time
4:00 pm - 6:00 pmPoster Session
6:00 pmDinner
7:30 pm - 9:30 pmPROTEIN RADICAL MECHANISMS
Discussion Leader: James M. Whittaker (Oregon Health and Science University, Beaverton, OR, USA)
7:30 pm - 8:00 pm Nigel S. Scrutton (The University of Manchester, United Kingdom)
"Dynamics of hydride transfer in enzyme systems"
8:00 pm - 8:10 pm Discussion
8:10 pm - 8:40 pm Brian M. Hoffman (Northwestern University, Evanston, IL, USA)
"Dynamical control of electron transfer between the nitrogenase component proteins"
8:40 pm - 8:50 pm Discussion
8:50 pm - 9:05 pmPoster Talk: Stefan Stoll (University of California Davis, CA, USA)
"Biliverdin substrate radical intermediate in PcyA, a cyanobacterial bilin reductase"
9:05 pm - 9:10 pmDiscussion
9:10 pm - 9:25 pmPoster Talk: Cecilia Tommos (University of Pennsylvania, Philadelphia, PA, USA)
"Understanding altered potentials and redox Bohr effects in tyrosine radical proteins"
9:25 pm - 9:30 pmDiscussion
TUESDAY
7:30 am - 8:30 amBreakfast
9:00 am - 12:30 pmCROSS-LINKED AND POST-TRANSLATIONALLY MODIFIED COFACTORS
Discussion Leader: Carrie M. Wilmot (University of Minnesota, Minneapolis, MN, USA)
9:00 am - 9:35 am Judith P. Klinman (University of California Berkeley, CA, USA)
"Quinocofactor biogenesis and function"
9:35 am - 9:45 am Discussion
9:45 am - 10:20 am Richard Magliozzo (Brooklyn College CUNY, Brooklyn, NY, USA)
"Role of the distal side Met-Tyr-Trp adduct in the catalase reaction of catalase-peroxidases"
10:20 am - 10:30 am Discussion
10:30 am Coffee Break
11:00 am - 11:15 am Poster Talk: Arwen Pearson (University of Leeds, United Kingdom)
"Oxygen OFF, Oxygen ON: Specific switch sites that control the oxygen delivery pathway in E. coli copper amine oxidase"
11:15 am - 11:20 am Discussion
11:20 am - 11:35 amPoster Talk: Lyndal Jensen (University of Minnesota, Minneapolis, MN, USA)
"In crystallo posttranslational modification within a MauG/pre-methylamine dehydrogenase complex"
11:35 am - 11:40 amDiscussion
11:40 am - 12:15 pmVictor L. Davidson (University of Mississippi, Jackson, MS, USA)
"A novel role for a diheme enzyme in tryptophan tryptophylquinone biosynthesis"
12:15 pm - 12:25 pmDiscussion
12:30 pmLunch
1:30 pm - 4:00 pmFree Time
4:00 pm - 6:00 pmPoster Session
6:00 pmDinner
7:30 pm - 9:30 pmB12 ENZYMES AND RIBOSWITCHES
Discussion Leader: Michael J. McPherson (University of Leeds, United Kingdom)
7:30 pm - 8:05 pm Kurt Warncke (Emory University, Atlanta, GA, USA)
"Protein and Solvent Dynamical Contributions to Radical Generation and Rearrangement Reactions in B12 Enzymes"
8:05 pm - 8:15 pm Discussion
8:15 pm - 8:35 pm Poster Talk: Carmen Gherasim (University of Michigan Medical Center, Ann Arbor, MI, USA)
"A bifunctional enzyme involved in cobalamin trafficking"
8:35 pm - 8:45 pm Discussion
8:45 pm - 9:20 pm Adam Roth (Yale University, New Haven, CT, USA)
"Riboswitch regulation of cofactor and coenzyme homeostasis"
9:20 pm - 9:30 pm Discussion
WEDNESDAY
7:30 am - 8:30 amBreakfast
9:00 am - 12:30 pmRADICAL SAM PROTEINS
Discussion Leader: Carsten Krebs (Penn State University, University Park, PA, USA)
9:00 am - 9:35 am Diana M. Downs (University of Wisconsin, Madison, WI, USA)
"Characterization of ThiC from Salmonella enterica"
9:35 am - 9:45 am Discussion
9:45 am - 10:20 am Steven E. Ealick (Cornell University, Ithaca, NY, USA)
"ThiC and Dph2 - two unpredicted SAM-dependent [4Fe-4S] enzymes"
10:20 am - 10:30 am Discussion
10:30 am Coffee Break
11:00 am - 11:35 am Joseph T. Jarrett (University of Hawaii Honolulu, HI, USA)
"Biotin synthase from Escherichia coli"
11:35 am - 11:45 am Discussion
11:45 am - 12:00 pmPoster Talk: Vahe Bandarian (University of Arizona, Tucson, AZ, USA)
"Radical mediated rearrangement in the biosynthesis of 7-deazapurine-containing compounds"
12:00 pm - 12:05 pmDiscussion
12:05 pm - 12:20 pmPoster Talk: Olivier Berteau (INRA, Jouy-en-Josas, France)
"Antibiotic resistance mediated by a radical AdoMet enzyme - Cfr a new type of RNA methyltransferase from Staphylococcus aureus"
12:20 pm - 12:25 pmDiscussion
12:30 pmLunch
1:30 pm - 4:00 pmFree Time
4:00 pm - 6:00 pmPoster Session
6:00 pmDinner
7:00 pm - 7:30 pmBusiness Meeting
(Nominations for the next Vice Chair; Fill out Conference Evaluation Forms; Discuss future Site & Scheduling preferences; Election of the next Vice Chair)
7:30 pm - 9:30 pmRADICAL SAM PROTEINS
Discussion Leader: Marc Fontecave (iRTSV, Grenoble, France)
7:30 pm - 8:05 pm Joan B. Broderick (Montana State University, Bozeman, MT, USA)
"Novel reactions in the radical-SAM superfamily"
8:05 pm - 8:15 pm Discussion
8:15 pm - 8:50 pm Squire J. Booker (Pennsylvania State University, University Park, PA, USA)
"Mechanistic insights into radical SAM dependent dehydrogenation reactions"
8:50 pm - 9:00 pm Discussion
9:00 pm - 9:20 pm Poster Talk: Peter Roach (University of Southampton, United Kingdom)
"Tyrosine lyase (ThiH) from E. coli: Kinetics, isotope effects and substrate analogues"
9:20 pm - 9:30 pm Discussion
THURSDAY
7:30 am - 8:30 amBreakfast
9:00 am - 12:30 pmOXYGENASES AND IRON SULFUR PROTEINS
Discussion Leader: Simon E.V. Phillips (Research Complex at Harwell, Harwell Science and Innovation Campus, Didcot, Oxon, United Kingdom)
9:00 am - 9:35 am Emma L. Raven (University of Leicester, Leicester, United Kingdom)
"Mechanism and reactivity in the heme dioxygenase enzymes"
9:35 am - 9:45 am Discussion
9:45 am - 10:20 am Antonio Pierik (Philipps-Universität Marburg, Germany)
"Function and biogenesis of iron-sulfur proteins"
10:20 am - 10:30 am Discussion
10:30 am Coffee Break
11:00 am - 11:35 am F. Wayne Outten (University of South Carolina, Columbia, SC, USA)
"Suf proteins and Fe-S cluster assembly pathways in bacteria"
11:35 am - 11:45 am Discussion
11:45 am - 12:00 pm Poster Talk: Pinghua Liu (Boston University, Boston, MA, USA)
"Mechanistic Studies of IspG and IspH in Prokaryotic Isoprenoid Biosynthesis"
12:00 pm - 12:05 pm Discussion
12:05 pm - 12:20 pm Poster Talk: Anabella Ivancich (CNRS & CEA-Saclay, Centre d'Etudes de Saclay, Gif-sur-Yvette, France)
"Multifrequency EPR spectroscopy evidence for a catalytically-active tryptophan radical, engineered in a heme peroxidase"
12:20 pm - 12:25 pm Discussion
12:30 pmLunch
1:30 pm - 4:00 pmFree Time
4:00 pm - 6:00 pmPoster Session
6:00 pmDinner
7:30 pm - 9:30 pmHYDROGENASES
Discussion Leader: John W. Peters (Montana State University, Bozeman, MT, USA)
7:30 pm - 8:10 pm Marc Fontecave (iRTSV, Grenoble, France)
"Radical-SAM chemistry in hydrogenase maturation: a novel C-S bond formation mechanism"
8:10 pm - 8:30 pm Discussion
8:30 pm - 9:10 pm Bärbel Friedrich (Humboldt University Berlin, Germany)
"Modifications of metal cofactors confer oxygen tolerance to NiFe hydrogenases"
9:10 pm - 9:30 pm Discussion
FRIDAY
7:30 am - 8:30 amBreakfast
9:00 amDepart

Last Updated: July 22, 2010
 
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