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Single Molecule Approaches to Biology
Gordon Research Conference

Dates

June 27 - July 2, 2010

Location

Renaissance Tuscany Il Ciocco
Lucca (Barga), Italy

Organizers

Chairs:
Nynke H. Dekker & W.E. Moerner

Vice Chairs:
Julio M. Fernandez & Stefan W. Hell

Meeting Description

The 2010 Gordon Conference on Single-Molecule Approaches to Biology focuses on cutting-edge research in single-molecule science. Tremendous technical developments have made it possible to detect, identify, track, and manipulate single biomolecules in an ambient environment or even in a live cell. Single-molecule approaches have changed the way many biological problems are addressed, and new knowledge derived from these approaches continues to emerge.

The ability of single-molecule approaches to avoid ensemble averaging and to capture transient intermediates and heterogeneous behavior renders them particularly powerful in elucidating mechanisms of biomolecular machines: what they do, how they work individually, how they work together, and finally, how they work inside live cells. The burgeoning use of single-molecule methods to elucidate biological problems is a highly multidisciplinary pursuit, involving both force- and fluorescence-based methods, the most up-to-date advances in microscopy, innovative biological and chemical approaches, and nanotechnology tools.

This conference seeks to bring together top experts in molecular and cell biology with innovators in the measurement and manipulation of single molecules, and will provide opportunities for junior scientists and graduate students to present their work in poster format and to exchange ideas with leaders in the field. A number of excellent poster presenters will be selected for short oral talks. Topics as diverse as single-molecule sequencing, DNA/RNA/protein interactions, folding machines, cellular biophysics, synthetic biology and bioengineering, force spectroscopy, new method developments, superresolution imaging in cells, and novel probes for single-molecule imaging will be on the program.

Additionally, the collegial atmosphere of this Conference, with programmed discussion sessions as well as opportunities for informal gatherings in the afternoons and evenings in the beauty of the Il Ciocco site in Tuscany, provides an avenue for scientists from different disciplines to interact and brainstorm and promotes cross-disciplinary collaborations directed toward compelling biological problems.

Contributors

Meeting Program

SUNDAY
4:00 pm - 8:00 pmArrival and Check-in (Check-in Desk Closed 6:00 pm - 7:45 pm)
6:00 pmDinner
7:30 pm - 7:40 pmWelcome / Introductory Comments by GRC Site Staff
7:40 pm - 9:30 pmSINGLE-MOLECULE SEQUENCING
7:40 pm - 7:50 pmDiscussion Leader: William E. Moerner (Stanford University)
7:50 pm - 8:15 pm Stephen Quake (Stanford University)
"Single Molecule Sequencing of a Human Genome"
8:15 pm - 8:25 pm Discussion
8:25 pm - 8:50 pm Jonas Korlach (Pacific Biosciences)
"Applications of Single-Molecule, Real-Time DNA Sequencing"
8:50 pm - 9:00 pm Discussion
9:00 pm - 9:25 pm Hagan Bayley (University of Oxford)
"Single-molecule chemistry in a protein nanoreactor"
9:25 pm - 9:30 pm Discussion
MONDAY
7:30 am - 8:30 amBreakfast
9:00 am - 12:30 pmDNA/RNA/PROTEIN INTERACTIONS
9:00 am - 9:10 amDiscussion Leader: Nynke Dekker (TU Delft)
9:10 am - 9:35 am Taekjip Ha (University of Illinois)
"A tale of three exonucleases"
9:35 am - 9:45 am Discussion
9:45 am - 10:10 am Michelle Wang (Cornell University)
"Torsional studies of single biological molecules"
10:10 am - 10:20 am Discussion
10:20 am - 10:32 am Irina Gopich (NIH)
"Theory and analysis of single-molecule photon trajectories and FRET efficiency histograms"
10:32 am - 10:35 am Discussion
10:35 am Coffee Break / Group Photo
11:05 am - 11:30 am Helmut Schiessel (Leiden University)
"Chromatin, a multi-scale jigsaw puzzle"
11:30 am - 11:40 am Discussion
11:40 am - 12:05 pm Johan Hofkens (KU Leuven)
"Single molecule spectroscopy of biopolymers"
12:05 pm - 12:15 pm Discussion
12:15 pm - 12:27 pm Borja Ibarra (Universidad Autonoma Madrid)
"Phi29 DNA polymerase actively separates DNA strands during DNA replication"
12:27 pm - 12:30 pm Discussion
12:30 pmLunch
1:30 pm - 4:00 pmFree Time
4:00 pm - 5:30 pmPoster Session One
5:30 pm - 7:30 pmFOLDING MACHINES
5:30 pm - 5:40 pmDiscussion Leader: Don Lamb (LMU, Munich)
5:40 pm - 6:05 pm Benjamin Schuler (University of Zürich)
"Probing chaperonin-mediated protein folding with single-molecule FRET"
6:05 pm - 6:13 pm Discussion
6:13 pm - 6:38 pmSander Tans (AMOLF)
"Protein folding pathways shaped by chaperones"
6:38 pm - 6:46 pm Discussion
6:46 pm - 7:11 pm Vijay Pande (Stanford University)
"Using new theoretical advances to improve the analysis of experimental single molecule data"
7:11 pm - 7:19 pm Discussion
7:19 pm - 7:28 pm Takashi Funatsu (University of Tokyo)
"Single-molecule characterization of the football-shaped GroEL-GroES complex using zero-mode waveguides"
7:28 pm - 7:30 pm Discussion
8:00 pmDinner
TUESDAY
7:30 am - 8:30 amBreakfast
9:00 am - 12:30 pmCELLULAR BIOPHYSICS
9:00 am - 9:10 amDiscussion Leader: Thomas Schmidt (Leiden University)
9:10 am - 9:35 am Michael Elowitz (California Institute of Technology)
"Dynamic signal encoding at the single-cell level"
9:35 am - 9:45 am Discussion
9:45 am - 10:10 am Paul Wiggins (Whitehead Institute)
"Folding a prokaryotic chromosome"
10:10 am - 10:20 am Discussion
10:20 am - 10:32 am Thomas Kuhlman (Princeton University)
"Genome Organization and its Consequences for Gene Regulation in E. coli"
10:32 am - 10:35 am Discussion
10:35 am Coffee Break
11:05 am - 11:30 am David Sherratt (University of Oxford)
"Dissecting the in vivo stoichiometry and architecture of active E. coli DNA replication machinery"
11:30 am - 11:40 am Discussion
11:40 am - 12:05 pm Maxime Dahan (Ecole Normale Supérieure, Paris)
"Addressing the nuclear dynamics and target search of transcription factors in eukaryotic cells"
12:05 pm - 12:15 pm Discussion
12:15 pm - 12:27 pm Jie Xiao (Johns Hopkins University)
"Probing the Dynamics of Gene Autoregulation in single E. coli cells"
12:27 pm - 12:30 pm Discussion
12:30 pmLunch
1:30 pm - 4:00 pmFree Time
4:00 pm - 5:30 pmPoster Session Two
5:30 pm - 7:30 pmSYNTHETIC BIOLOGY AND BIOENGINEERING
5:30 pm - 5:40 pmDiscussion Leader: Hermann Gaub (LMU Munich)
5:40 pm - 6:05 pm William Shih (Harvard University)
"Self-assembly of DNA into nanoscale three-dimensional shapes"
6:05 pm - 6:15 pm Discussion
6:15 pm - 6:40 pmJörg Fitter (Research Center Jülich)
"Single molecule fluorescence studies on proteins encapsulated in surface tethered polymersomes"
6:40 pm - 6:50 pm Discussion
6:50 pm - 7:02 pm Victoria Birkedal (Aarhus University)
"Single molecule FRET microscopy studies of DNA nanostructures"
7:02 pm - 7:05 pm Discussion
7:05 pm - 7:17 pm Charina Choi (University of California, Berkeley)
"A nanocrystal strain gauge for optical detection of biomechanical forces"
7:17 pm - 7:20 pm Discussion
8:00 pmDinner
WEDNESDAY
7:30 am - 8:30 amBreakfast
9:00 am - 12:30 pmFORCE SPECTROSCOPY
9:00 am - 9:10 amDiscussion Leader: Julio Fernandez (Columbia University)
9:10 am - 9:35 am Michael Woodside (University of Alberta)
"Force spectroscopy of adenine riboswitch folding"
9:35 am - 9:45 am Discussion
9:45 am - 10:10 am Erik Schäffer (TU Dresden)
"Nanomechanics with Optical Tweezers: Kinesin Motors Work against Protein Friction"
10:10 am - 10:20 am Discussion
10:20 am - 10:32 am Jan Lipfert (TU Delft)
"Novel Approaches using Magnetic Tweezers"
10:32 am - 10:35 am Discussion
10:30 am Coffee Break
11:05 am - 11:30 am Klaus Schulten (University of Illinois)
"New force-extension-structure characteristics of protein and DNA discovered through simulation and observation"
11:30 am - 11:40 am Discussion
11:40 am - 12:05 pm Jasna Brujic (New York University)
"Single molecule force spectroscopy reveals hidden complexities in the structure and dynamics of proteins"
12:05 pm - 12:15 pm Discussion
12:15 pm - 12:27 pm Matthew Larson (Stanford University)
"The mechanism of hairpin-mediated transcription termination"
12:27 pm - 12:30 pm Discussion
12:30 pmLunch
1:30 pm - 4:00 pmFree Time
4:00 pm - 5:30 pmPoster Session Three
5:30 pm - 7:30 pmNEW FRONTIERS IN METHODS
5:30 pm - 5:40 pmDiscussion Leader: Taekjip Ha (University of Illinois)
5:40 pm - 6:05 pm Haw Yang (Princeton University)
"Real-Time 3D Single-Particle Tracking Spectroscopy and Imaging"
6:05 pm - 6:13 pm Discussion
6:13 pm - 6:38 pm Adam Cohen (Harvard University)
"Optical recording of electrical spikes in E. coli using a new class of voltage-sensitive fluorescent proteins"
6:38 pm - 6:46 pm Discussion
6:46 pm - 7:11 pm Tom Perkins (JILA/University of Colorado)
"Precision force spectroscopy: a new window on the dynamics of folding and refolding membrane proteins"
7:11 pm - 7:19 pm Discussion
7:19 pm - 7:28 pm Randall Goldsmith (Stanford University)
"Watching conformational- and photodynamics of single fluorescent proteins in solution"
7:28 pm - 7:30 pm Discussion
7:30 pm - 8:00 pmBusiness Meeting
(Nominations for the next Vice Chair; Fill out Conference Evaluation Forms; Discuss future Site & Scheduling preferences; Election of the next Vice Chair)
8:00 pmDinner
THURSDAY
7:30 am - 8:30 amBreakfast
9:00 am - 12:30 pmSUPERRESOLUTION
9:00 am - 9:10 amDiscussion Leader: Stefan Hell (MPI Göttingen)
9:10 am - 9:35 am Xiaowei Zhuang (Harvard)
"Superresolution imaging"
9:35 am - 9:45 am Discussion
9:45 am - 10:10 am Christian Eggeling (MPI Göttingen)
"Details of nanoscale membrane dynamics revealed by far-field fluorescence nanoscopy"
10:10 am - 10:20 am Discussion
10:20 am - 10:32 am Ann McEvoy (University of California, Berkeley)
"Spatial organization in bacteria studied with super-resolution fluorescence microscopy"
10:32 am - 10:35 am Discussion
10:35 am Coffee Break
11:05 am - 11:30 am Jörg Bewersdorf (Yale University)
"New developments and applications in STED microscopy and FPALM"
11:30 am - 11:40 am Discussion
11:40 am - 12:05 pm Raimund Ober (University of Texas, Southwestern)
"Multifocal plane microscopy for imaging single molecules in 3D"
12:05 pm - 12:15 pm Discussion
12:15 pm - 12:27 pm Cristina Flors (University of Edinburgh)
"Super-resolution microscopy of DNA"
12:27 pm - 12:30 pm Discussion
12:30 pmLunch
1:30 pm - 4:00 pmFree Time
4:00 pm - 5:30 pmPoster Session Four
5:30 pm - 7:30 pmPROBE DEVELOPMENT FOR SINGLE-MOLECULE EXPERIMENTS
5:30 pm - 5:40 pmDiscussion Leader: Xiaowei Zhuang (Harvard University)
5:40 pm - 6:05 pm George Patterson (National Institutes of Health)
"Development of fluorescent proteins for single molecule localization techniques"
6:05 pm - 6:13 pm Discussion
6:13 pm - 6:38 pm Alice Ting (Massachusetts Institute of Technology)
"Enzymatic ligation of probes for single molecule and super-resolution imaging in cells"
6:38 pm - 6:46 pm Discussion
6:46 pm - 6:55 pm Fabien Pinaud (Ecole Normale Supérieure)
"Complementation Activated Light Microscopy with split-Fluorescent Proteins (CALM-sFP): A new approach for targeting and imaging single molecules in living cells"
6:55 pm - 6:57 pm Discussion
6:57 pm - 7:22 pm Philip Tinnefeld (University of Munich)
"Make them blink: Controlling Fluorescent Dyes for Single-Molecule and Superresolution Microscopy"
7:22 pm - 7:30 pm Discussion
8:00 pmDinner
FRIDAY
7:30 am - 8:30 amBreakfast
9:00 amDepart

Funding for this conference was made possible (in part) by 1 R13 GM 093362-01 from the National Institute of General Medical Sciences. The views expressed in written conference materials or publications and by speakers and moderators do not necessarily reflect the official policies of the Department of Health and Human Services; nor does mention by trade names, commercial practices, or organizations imply endorsement by the U.S. Government.
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