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Three Dimensional Electron Microscopy
June 20-25, 2010
Renaissance Tuscany Il Ciocco Resort
Lucca (Barga), Italy
Chair:
Holger Stark

Vice Chair:
Michael Radermacher

The 2010 Gordon Research Conference on Three-Dimensional Electron Microscopy will highlight advances in three-dimensional structure determination of cells and biological macromolecules by electron tomography, 2D electron crystallography and single particle cryo-EM. The conference will focus on recent methodological advances in electron microscopic hardware and detector development as well as new image analysis/software methods for 3D structure determination. Current limitations in the available technology will be discussed in the highly interactive and collegial atmosphere of this Gordon Conference. The conference includes a mix of long and short presentations and poster sessions that will foster stimulating interactions between participants, comprising both senior and junior investigators, as well as postdoctoral fellows and graduate students. There will be invited plenary lectures, contributed poster presentations and generous amounts for discussions as well as informal gatherings in the evenings in the stimulating Tuscany ambiance.

SUNDAY
4:00 pm - 8:00 pmArrival and Check-in (Check-in Desk Closed 6:00 pm - 7:45 pm)
6:00 pmDinner
7:30 pm - 7:40 pmWelcome / Introductory Comments by GRC Site Staff
7:40 pm - 9:30 pmAlternative Ways of Structure Determination and Computation
Discussion Leader: Holger Stark (MPI Göttingen, Germany)
7:40 pm - 8:20 pm Gerard Bricogne (Global Phasing Ltd., Cambridge, UK)
"Bayesian Approaches to 3D structure Determination: a Witness's Report from X-ray Crystallography"
8:20 pm - 8:35 pm Discussion
8:35 pm - 9:15 pmSimone Techert (MPI Göttingen, Germany)
"The Study of Structures with Ultrashort X-ray Pulses Provided by Free Electron Lasers"
9:15 pm - 9:30 pm Discussion
MONDAY
7:30 am - 8:30 amBreakfast
9:00 am - 12:30 pmSingle particle CryoEM at Atomic or Near-Atomic Resolutions
Discussion Leader: Hong Zhou (UCLA, USA)
9:00 am - 9:20 am Wah Chiu (Baylor College of Medicine, Houston, USA)
"CryoEM of Chaperonins"
9:20 am - 9:30 am Discussion
9:30 am - 9:50 am Hong Zhou (UCLA, USA)
"Atomic Resolution Structure Determination of Large Viruses"
9:50 am - 10:00 am Discussion
10:00 am Coffee Break / Group Photo
10:45 am - 11:05 am Marin van Heel (Imperial College London, UK)
"X-ray crystallography, the End of an Era?"
11:05 am - 11:15 am Discussion
11:15 am - 11:35 am Yifan Cheng (UC San Francisco, USA)
"Attempting high resolution single particle cryoEM with an 8K camera, an example with 20S proteasome"
11:35 am - 11:45 am Discussion
11:45 am - 12:05 pm Matthias Wolf (BCMP, Harvard Medical School)
"Lessons learned from high resolution virus reconstructions"
12:05 pm - 12:15 pm Discussion
12:30 pmLunch
1:30 pm - 4:00 pmFree Time
4:00 pm - 5:30 pmPoster Session
5:30 pm - 7:30 pmAutomation and How to Deal with Very Large Datasets
Discussion Leader: Bridget Carragher (The Scripps Res. Inst., La Jolla, CA, USA)
5:30 pm - 5:50 pmAnchi Cheng (The Scripps Res. Inst., La Jolla, CA, USA)
"Automating image acquisition and microscope alignment"
5:50 pm - 6:00 pm Discussion
6:00 pm - 6:20 pmDavid Stokes (NYU Langone Medical Center, NY, USA)
"2D Crystal Screening Automation"
6:20 pm - 6:30 pm Discussion
6:30 pm - 6:50 pmBurkhard Heisen (MPI Göttingen, Germany)
"File formats for Large Image Datasets and Parallel Processing"
6:50 pm - 7:00 pm Discussion
7:00 pm - 7:20 pm Farshid Moussavi (Stanford University)
"3D Segmentation of Cell Boundaries from Cryogenic Electron Tomograms"
7:20 pm - 7:30 pm Discussion
8:00 pmDinner
TUESDAY
7:30 am - 8:30 amBreakfast
9:00 am - 12:30 pmStructural and Compositional Imaging of Heterogeneous Assemblies
Discussion Leader: Sriram Subramaniam (NCI Bethesda, MD, USA)
9:00 am - 9:20 amSjors Scheres (MRC-LMB, Cambridge, UK )
"Reference-free sub-tomogram averaging: expectation maximization for an incomplete data problem"
9:20 am - 9:30 am Discussion
9:30 am - 9:50 am Fernando Amat (Stanford University)
"Improving subtomogram averaging with adaptive Fourier coefficient thresholding and Markov random fields"
9:50 am - 10:00 am Discussion
10:00 am Coffee Break
10:45 am - 11:05 am Ohad Medalia (Ben Gurion University, Beer Sheva, Israel)
"The Architecture of Integrin-Mediated Focal Adhesion by Cryo-Electron Tomography"
11:05 am - 11:15 am Discussion
11:15 am - 11:35 am John Henry J. Scott (NIST, Gaithersburg, MD USA)
"Enhancing 3DEM with Chemical Information: Hyperspectral Analysis in the TEM"
11:35 am - 11:45 am Discussion
11:45 am - 12:05 pm Sriram Subramaniam (NCI Bethesda, MD, USA)
"Progress and challenges in 3D compositional mapping of eukaryotic cells"
12:05 pm - 12:15 pm Discussion
12:30 pmLunch
1:30 pm - 4:00 pmFree Time
4:00 pm - 5:30 pmPoster Session
5:30 pm - 7:30 pmSelected Poster Presentations I
Discussion Leader: Michael Radermacher (University of Vermont, USA)
(Speakers will be identified for short talks at the conference)
8:00 pmDinner
WEDNESDAY
7:30 am - 8:30 amBreakfast
9:00 am - 12:30 pmSingle Particle Cryo-EM of "Challenging" Macromolecules
Discussion Leader: Eva Nogales (University of Berkeley., CA, USA)
9:00 am - 9:20 amEva Nogales (HHMI/UCB/LBNL)
"Dealing with macromolecular flexibility and how flexibility matters"
9:20 am - 9:30 am Discussion
9:30 am - 9:50 amAshwin Chari (University of Wuerzburg, Germany)
"Optimizing the stability of macromolecular complexes"
9:50 am - 10:00 am Discussion
10:00 am Coffee Break
10:45 am - 11:05 am Fred Sigworth (Yale University., New Haven, USA)
"Membrane proteins in membranes, as single particles"
11:05 am - 11:15 am Discussion
11:15 am - 11:35 am Tanvir Shaikh (Wadsworth Center, Albany, NY, USA)
"Association of ribosomal subunits as studied by time-resolved cryo-EM"
11:35 am - 11:45 am Discussion
11:45 am - 12:05 pm Abbas Ourmazd (University of Wisconsin - Milwaukee)
"Structure and evolution by manifold mapping"
12:05 pm - 12:15 pm Discussion
12:30 pmLunch
1:30 pm - 4:00 pmFree Time
4:00 pm - 5:30 pmPoster Session
5:30 pm - 7:30 pmCorrelated Microscopy
Discussion Leader: Abraham Koster (Leiden University, The Netherlands)
5:30 pm - 5:50 pm Linda van Driel (Leiden University, The Netherlands)
"Integrated Cryo Correlative Light Electron Microscopy"
5:50 pm - 6:00 pm Discussion
6:00 pm - 6:20 pm Paul Verkade (University of Bristol, UK)
"The use of High Pressure Freezing in CLEM-Tomography experiments"
6:20 pm - 6:30 pm Discussion
6:30 pm - 6:50 pm Juergen Plitzko (MPI for Biochemistry, Martinsried, Germany)
"Challenges and capabilities of hybrid experimental approaches for three-dimensional electron microscopy"
6:50 pm - 7:00 pm Discussion
7:00 pm - 7:20 pm Ariane Briegel (Caltech, CA, USA)
"How correlated fluorescent light microscopy and electron cryotomography has impacted our understanding of bacterial ultrastructure"
7:20 pm - 7:30 pm Discussion
7:30 pm - 8:00 pmBusiness Meeting
(Nominations for the next Vice Chair; Fill out Conference Evaluation Forms; Discuss future Site & Scheduling preferences; Election of the next Vice Chair)
8:00 pmDinner
THURSDAY
7:30 am - 8:30 amBreakfast
9:00 am - 12:30 pmNew Hardware for Electron Microscopy
Discussion Leader: Henning Stahlberg (Affiliation)
9:00 am - 9:20 am Henning Stahlberg (University Basel, Switzerland)
9:20 am - 9:30 am Discussion
9:30 am - 9:50 amRasmus Schröder (University Heidelberg, Germany)
"Aberration Correction in Biology"
9:50 am - 10:00 am Discussion
10:00 am Coffee Break
10:45 am - 11:05 am Ute Kaiser (University Ulm, Germany)
"Prospects of Low Voltage Electron Micorscopy for Beam-Sensitive Materials"
11:05 am - 11:15 am Discussion
11:15 am - 11:35 am Bob Glaeser (University of Berkeley, California, USA)
"Options For In-focus Phase Contrast Provided By Additional Relay Optics"
11:35 am - 11:45 am Discussion
11:45 am - 12:05 pm Kuniaki Nagayama (Okazaki Institute for Integrative Bioscience, Okazaki, Japan)
"An Electron-Photon Hybrid Microscope for Real-time Correlative Microscopy"
12:05 pm - 12:15 pm Discussion
12:30 pmLunch
1:30 pm - 4:00 pmFree Time
4:00 pm - 5:30 pmPoster Session
5:30 pm - 7:30 pmSelected Poster Presentations II
Discussion Leader: Michael Radermacher (University of Vermont, USA)
(Speakers will be identified for short talks at the conference)
8:00 pmDinner
FRIDAY
7:30 am - 8:30 amBreakfast
9:00 amDepart
Funding for this conference was made possible (in part) by R13 GM085826 from National Institute of General Medical Sciences. The views expressed in written conference materials or publications and by speakers and moderators do not necessarily reflect the official policies of the Department of Health and Human Services; nor does mention by trade names, commercial practices, or organizations imply endorsement by the U.S. Government.
 
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