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Conference Program
 
Chromosome Dynamics
July 10-15, 2011
Mount Snow Resort
West Dover, VT
Chair:
Susan T. Lovett

Vice Chair:
Frank Uhlmann

The semi-annual Chromosome Dynamics GRC attracts participants from across the globe, working with bacterial, archaeal or eukaryotic systems. The Chromosome Dynamics Gordon Conference evolved from a meeting that was narrowly focused on bacterial plasmid replication and segregation. As the scientific understanding of larger bacterial chromosomes and eukaryotic chromosomes blossomed, the Bacterial Plasmid GRC expanded its focus to bring new topics into the program.

Unlike many excellent specialty meetings where experts in eukaryotic or prokaryotic DNA replication, recombination, or transcription delve in to the intricacies of their special realm, the Chromosome Dynamics GRC takes a different tack. It includes interested participants with very different specialties including cell visualization, structural biology, genetics and biochemistry, who share the common goal of understanding chromosome dynamics and the mechanisms that organize large DNA molecules. This is critical because replication, recombination, transposition, mutation, gene transcription/silencing, gene order on chromosomes, and genome segregation are all phenomena that depend on aspects of chromosome dynamics.

The conference brings together a group of scientists at the frontiers of their fields and talks present unpublished work on a variety of processes and model systems. The meeting provides an intimate atmosphere for junior scientists and students to present their work and exchange ideas through poster sessions and informal discussions. Short talks in each session will include those chosen from the abstracts.

Breakout work on chromosomes from viruses to man has been announced at past meetings. Highlights included: new biochemistry and cell biology of condensins and cohesins, the role of actin-related proteins in bacterial chromosome segregation, new roles for telomeres in replication and repair, single-molecule studies of chromatin remodeling proteins and new roles for RNA in controlling epigenetic gene regulation and centromere function.


Contributors

SUNDAY
2:00 pm - 9:00 pmArrival and Check-in (Office Closed 6:00 pm - 7:00 pm)
6:00 pmDinner
7:30 pm - 7:40 pmWelcome / Introductory Comments by GRC Site Staff
7:40 pm - 9:30 pmFUNCTIONAL ORGANIZATION OF CHROMOSOMES
Discussion Leader: Sue Lovett (Brandeis University)
7:40 pm - 8:20 pm David Sherratt (University of Oxford)
"Watching molecules one by one in DNA replication and chromosome segregation"
8:20 pm - 8:35 pm Discussion
8:35 pm - 9:15 pm Susan Gasser (Friendrich Miescher Institute for Biomedical Reseach)
"The biology of DNA dynamics: how remodelers facilitate repair"
9:15 pm - 9:30 pm Discussion
MONDAY
7:30 am - 8:30 amBreakfast
9:00 am - 12:30 pmVIEWS OF CHROMOSOMES AND CHROMOSOMAL PROTEINS
Discussion Leader: David Sherratt (Oxford University)
9:00 am - 9:20 am James Berger (University of California-Berkeley)
"Molecular mechanisms for initiating DNA replication"
9:20 am - 9:30 am Discussion
9:30 am - 9:50 am Nancy Kleckner (Harvard University)
"The living E.coli nucleoid: structure, mechanics and short-time-scale dynamics"
9:50 am - 10:00 am Discussion
10:00 am - 10:20 am Xiaowei Zhuang (Harvard University)
"Single-molecule studies of chromosome dynamics and remodeling"
10:20 am - 10:30 am Discussion
10:30 am - 11:00 am Group Photo / Coffee Break
11:00 am - 11:20 am Maria Spies (University of Illinois-Urbana Champaign)
"Sorting posttranslationally modified DNA repair machines in single-molecule studies"
11:20 am - 11:30 am Discussion
11:30 am - 11:50 am Kiyoshi Muzuuichi (NIDDK)
"Visualizing the assembly and disassembly of segregation machines"
11:50 am - 12:00 pm Discussion
12:00 pm - 12:10 pm Kazuhiro Maeshima (National Institute of Genetics, Mishima)
"Mitotic chromosome structure: irregular folding of nucleosome fibers without the 30-nm chromatin fiber"
12:10 pm - 12:15 pm Discussion
12:15 pm - 12:25 pm Thomas Maresca (University of Massachusetts, Amherst)
"Regulation of kinetochore-microtubule attachment by mitotic forces"
12:25 pm - 12:30 pm Discussion
12:30 pmLunch
1:30 pm - 4:00 pmFree Time
4:00 pm - 6:00 pmPoster Session
6:00 pmDinner
7:30 pm - 9:30 pmCHROMOSOME INTERACTIONS
Discussion Leader: James Berger (University of California-Berkeley)
7:30 pm - 7:50 pm Jennifer Fung (UCSF)
"Kinetics of chromosome synapsis during meiosis"
7:50 pm - 8:00 pm Discussion
8:00 pm - 8:20 pm Aaron Straight (Stanford University)
"In vitro centromere and kinetochore assembly on reconstituted centromeric chromatin"
8:20 pm - 8:30 pm Discussion
8:30 pm - 8:50 pm Abby Dernburg (University of California-Berkeley)
"Dynamics of homolog interactions during meiosis"
8:50 pm - 9:00 pm Discussion
9:00 pm - 9:10 pm Jorge Schvartzman (Centro de Investigaciones Biologicas, CSIC)
"Topo IV is the topoisomerase that knots and unknots sister duplexes during DNA replication"
9:10 pm - 9:15 pm Discussion
9:15 pm - 9:25 pm Kingsley Boateng (NIDDK)
"Novel early homolog pairing independent of meiotic double-strand breaks and homologous recombination during mammalian meiosis"
9:25 pm - 9:30 pm Discussion
TUESDAY
7:30 am - 8:30 amBreakfast
9:00 am - 12:30 pmGENOME STRUCTURE AND EVOLUTION
Discussion Leader: Abby Dernburg (University of California-Berkeley)
9:00 am - 9:20 am David Pellman (Harvard Medical School)
"Chromosome breaks from errors in mitosis"
9:20 am - 9:30 am Discussion
9:30 am - 9:50 am Ting Wu (Harvard Medical School)
"Looking at homolog pairing"
9:50 am - 10:00 am Discussion
10:00 am - 10:20 am Oliver Rando (University of Massachusetts-Worcester)
"Chromatin dynamics in fungi: from 15 minutes to 1 billion years"
10:20 am - 10:30 am Discussion
10:30 am - 11:00 am Coffee Break
11:00 am - 11:20 am Stuart Austin (NCI/Frederick)
"Dynamic mapping of the replicating E.coli chromosome shows it to be a hollow tube with an origin segregation machine within its central void"
11:20 am - 11:30 am Discussion
11:30 am - 11:50 am Rachel O'Neill (University of Connecticut)
"The role of ncRNA in centromeres: lessons from marsupials"
11:50 am - 12:00 pm Discussion
12:00 pm - 12:10 pm Dhruba Chattoraj (NIH)
"Replication of Vibrio cholerae chromosome II: Features that endow a plasmid-like origin to function in a cell-cycle specific fashion"
12:10 pm - 12:15 pm Discussion
12:15 pm - 12:25 pm John Marko (Northwestern University)
"Micromechanics and structure of mitotic chromosomes"
12:25 pm - 12:30 pm Discussion
12:30 pmLunch
1:30 pm - 4:00 pmFree Time
4:00 pm - 6:00 pmPoster Session
6:00 pmDinner
7:30 pm - 9:30 pmCHROMOSOME ARCHITECTURE
Discussion Leader: Oliver Rando (University of Massachusetts-Worcester)
7:30 pm - 7:50 pm Rebecca Heald (University of California-Berkeley)
"Mitotic chromosome compaction and scaling in Xenopus"
7:50 pm - 8:00 pm Discussion
8:00 pm - 8:20 pm Olivier Espeli (CNRS)
"Control of sister chromatid cohesion in bacteria"
8:20 pm - 8:30 pm Discussion
8:30 pm - 8:50 pm Job Dekker (University of Massachusetts-Worcester)
"Folding principles of genomes"
8:50 pm - 9:00 pm Discussion
9:00 pm - 9:10 pm Kerstin Bystricky-Weiss (University of Toulouse)
"Dissecting chromatin conformation and dynamics in 3D"
9:10 pm - 9:15 pm Discussion
9:15 pm - 9:25 pm Christopher Weitzel (Brandeis University)
"Transcription-dependent, H-NS mediated organization of the E. coli chromosome into a toroid"
9:25 pm - 9:30 pm Discussion
WEDNESDAY
7:30 am - 8:30 amBreakfast
9:00 am - 12:30 pmCHROMOSOME TOPOLOGY AND REPLICATION
Discussion Leader: Olivier Espeli (CNRS)
9:00 am - 9:20 am Joaquim Roca Bosch (Instituto de Biologia Molecular de Barcelona, CSIC)
"DNA torsional stress in chromatin biology"
9:20 am - 9:30 am Discussion
9:30 am - 9:50 am Terry Orr-Weaver (MIT)
"Differential DNA replication: Developmental control of replication initiation and fork progression"
9:50 am - 10:00 am Discussion
10:00 am - 10:20 am Pat Higgins (University of Alabama Medical School)
"The Impact of Gyrase, Topo I, and mobile DNA on the DNA topology of highly transcribed genes"
10:20 am - 10:30 am Discussion
10:30 am - 11:00 am Coffee Break
11:00 am - 11:20 am Alan Leonard (Florida Institute of Technology)
"OriC nucleotide sequences direct assembly of pre-replication complexes in E. coli"
11:20 am - 11:30 am Discussion
11:30 am - 11:50 am Ken Marians (Memorial Sloan Kettering Institute)
"Physical and functional interaction between the condensin MukB and the decatenase Topoisomerase IV"
11:50 am - 12:00 pm Discussion
12:00 pm - 12:10 pm Thomas Beattie (University of Oxford)
"The role of the DNA sliding clamp in lagging strand DNA replication"
12:10 pm - 12:15 pm Discussion
12:15 pm - 12:25 pm Steven Jacobsen (University of California, Los Angeles)
"Regulation of heterochromatic DNA replication by histone H3 lysine 27 methyltransferases"
12:25 pm - 12:30 pm Discussion
12:30 pmLunch
1:30 pm - 4:00 pmFree Time
4:00 pm - 6:00 pmPoster Session
6:00 pmDinner
7:00 pm - 7:30 pmBusiness Meeting
Nominations for the next Vice Chair; Fill out Conference Evaluation Forms; Discuss future Site & Scheduling preferences; Election of the next Vice Chair
7:30 pm - 9:30 pmCHROMOSOME REPAIR AND RECOMBINATION
Discussion Leader: Terry Orr-Weaver (MIT)
7:30 pm - 7:50 pm James Haber (Brandeis University)
"Repair of a broken chromosome"
7:50 pm - 8:00 pm Discussion
8:00 pm - 8:20 pm Virginia Zakian (Princeton University)
"Role of Pif1 family helicases in genome maintenance"
8:20 pm - 8:30 pm Discussion
8:30 pm - 8:40 pm Jinmin Gao (Harvard Medical School)
"Maintaining genomic integrity and promoting chromosome synapsis in C. elegans"
8:40 pm - 8:45 pm Discussion
8:45 pm - 8:55 pm Syam Anand (University of Pittsburgh)
"Translocation of PcrA helicase is insufficient to displace RecA from DNA"
8:55 pm - 9:00 pm Discussion
9:00 pm - 9:10 pm Liisa Kauppi (Sloan-Kettering Institute)
"How much is enough? Reduced meiotic double-strand breaks result in chromosome pairing defects in mice"
9:10 pm - 9:15 pm Discussion
9:15 pm - 9:25 pm Yasushi Hiraoka (Osaka University)
"Meiosis-specific non-coding RNA mediates robust pairing of homologous chromosomes in meiosis"
9:25 pm - 9:30 pm Discussion
THURSDAY
7:30 am - 8:30 amBreakfast
9:00 am - 12:30 pmCHROMOSOME SEGREGATION
Discussion Leader: Virginia Zakian (Princeton University)
9:00 am - 9:20 am Doug Koshland (University of California-Berkeley)
"When RNA goes rogue"
9:20 am - 9:30 am Discussion
9:30 am - 9:50 am Frank Uhlmann (Cancer Research UK)
"A molecular view of mitotic chromosome condensation"
9:50 am - 10:00 am Discussion
10:00 am - 10:20 am David Rudner (Harvard Medical School)
"Chromosome remodeling and translocation during sporulation in Bacillus subtilis"
10:20 am - 10:30 am Discussion
10:30 am - 11:00 am Coffee Break
11:00 am - 11:20 am Camilla Sjogren (Karolinska Institute)
"The Smc5/6 complex and chromosome topology"
11:20 am - 11:30 am Discussion
11:30 am - 11:50 am Hironori Funabiki (Rockefeller University)
"Roles of histone modifications during mitosis"
11:50 am - 12:00 pm Discussion
12:00 pm - 12:10 pm Barbara Funnell (University of Toronto)
"P1 plasmid partition involves an ATP-driven association between the plasmid and the bacterial nucleoid via a dynamic adaptor complex"
12:10 pm - 12:15 pm Discussion
12:15 pm - 12:25 pm Christian Haering (EMBL)
"Condensin structures chromosomal DNA through topological links"
12:25 pm - 12:30 pm Discussion
12:30 pmLunch
1:30 pm - 4:00 pmFree Time
4:00 pm - 6:00 pmPoster Session
6:00 pmDinner
7:30 pm - 9:30 pmTELOMERES AND CENTROMERES
Discussion Leader: Frank Uhlmann (Cancer Research UK)
7:30 pm - 7:50 pm Julie Cooper (Cancer Research UK)
"Telomeres and the challenges to chromosome integrity"
7:50 pm - 8:00 pm Discussion
8:00 pm - 8:20 pm Joachim Lingner (Swiss Institute for Experimental Cancer Research - ISREC)
"Regulation of telomerase at chromosome ends"
8:20 pm - 8:30 pm Discussion
8:30 pm - 8:50 pm Gary Karpen (Lawrence Berkeley Laboratory)
"Chromatin regulation of genome stability"
8:50 pm - 9:00 pm Discussion
9:00 pm - 9:10 pm Laura Burrack (University of Minnesota)
"Flexibility of kinetochore size and position in Candida albicans"
9:10 pm - 9:15 pm Discussion
9:15 pm - 9:25 pm Katsumi Kitagawa (Research Institute at Nationwide Children's Hospital)
"CENP-A ubiquitylation by the CUL4-RBX1-COPS8 E3 ligase is required for CENP-A loading at centromeres"
9:25 pm - 9:30 pm Discussion
FRIDAY
7:30 am - 8:30 amBreakfast
9:00 amDeparture

 
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