Gordon Research Conferences
PI_Conferences PI_Conferences_Past
Conference Program
 
 
 
QUICK SEARCH
GO
[ advanced search ]
 
 
 
Three Dimensional Electron Microscopy
June 26 - July 1, 2011
Colby-Sawyer College
New London, NH
Chair:
Michael Radermacher

Vice Chair:
Christian MT. Spahn

This 2011 Gordon conference will provide a forum for discussion of the latest progress in three-dimensional electron microscopy of macromolecular assemblies and sub-cellular components. Progress has been made in three major areas:

  • Towards atomic resolution for 3D structures of periodic, symmetrical and asymmetrical particles by a combination of 3D electron microscopy, X-ray structure fitting and structure prediction using EM density maps as constraints.
  • Towards understanding the dynamics of biological function by combining the structures of conformations of macromolecules in different functional states.
  • Towards the understanding of cell architecture and the structure of sub-cellular components by improving techniques for electron tomography and 3D averaging.

Progress in all three areas was facilitated by new developments for specimen preparation and labeling, by improved microscope technology and imaging techniques and by the creation of new the image processing algorithms. This conference will offer ample opportunity to discuss all aspects of the latest developments in these areas and to discover the challenges that lie ahead.

Contributors
SUNDAY
2:00 pm - 9:00 pmArrival and Check-in (Office Closed 6:00 pm - 7:00 pm)
6:00 pmDinner
7:30 pm - 7:40 pmWelcome / Introductory Comments by GRC Site Staff
7:40 pm - 9:30 pmImaging and Information
7:40 pm - 7:50 pm Discussion Leader: Michael Radermacher (University of Vermont, Burlington,VT)
"Introduction and Overview"
7:50 pm - 8:25 pm Robert Glaeser (Lawrence Berkeley National Laboratory, Berkeley, CA)
"Reaching the Information Limit in Cryo-EM of Biological Macromolecules - Experimental Aspects"
8:25 pm - 8:40 pm Discussion
8:40 pm - 9:15 pm Reiner Hegerl (Max-Planck-Institute for Biochemistry, Martinsried, Germany)
"Structural Information: A new Criterion for Image Quality? An Attempt"
9:15 pm - 9:30 pm Discussion
MONDAY
7:30 am - 8:30 amBreakfast
8:30 amGroup Photo
9:00 am - 12:30 pm3D Reconstruction of Single Particles, Methods and Results I
9:00 am - 9:10 am Discussion Leader: John Rubinstein (The Hospital for Sick Children, Toronto, Canada)
"Introduction and Overview"
9:10 am - 9:35 am Giovanni Cardone (NIH, Laboratory of Structural Biology Research, Bethesda, MD)
"Local Variation in Resolution in Cryo-EM Density Maps"
9:35 am - 9:50 am Discussion
9:50 am - 10:30 am Coffee Break
10:30 am - 10:55 am David Barford (Institute of Cancer Research, London, UK)
"Structural Basis for the Subunit Assembly of the Anaphase Promoting Complex"
10:55 am - 11:10 am Discussion
11:10 am - 11:35 am Teresa Ruiz (University of Vermont, Burlington, VT)
"High Resolution Structure of S. cerevisiae Phosphofructokinase: A Combination of Cryo 3D Reconstruction, Modeling and Docking"
11:35 am - 11:50 am Discussion
11:50 am - 12:15 pm John Rubinstein (The Hospital for Sick Children, Toronto, Canada)
"Cryo-EM Map Refinement for Small Membrane Protein Complexes"
12:15 pm - 12:30 pm Discussion
12:30 pmLunch
1:30 pm - 4:00 pmFree Time
4:00 pm - 6:00 pmPoster Session
6:00 pmDinner
7:30 pm - 9:30 pmMultiresolution: Fitting of X-ray Models to 3D EM Data
7:30 pm - 7:45 pm Discussion Leader: Edward Morris (Institute of Cancer Research, London, UK)
"Introduction and Overview"
7:45 pm - 8:10 pm Tom Goddard (University of California, San Francisco, CA)
"Packing Macromolecules in Medium Resolution (10-20Å) Maps with UCSF Chimera"
8:10 pm - 8:20 pm Discussion
8:20 pm - 8:45 pm Michael S. Chapman (Oregon Health & Science University, Portland, OR)
"Hybrid Structure Refinement: Choosing the Algorithm for Greatest Precision"
8:45 pm - 8:55 pm Discussion
8:55 pm - 9:20 pm Karen Lasker (University of California, San Francisco, CA)
"Structural Modeling of Macromolecular Assemblies by Alignment of Interaction Networks to Electron Microscopy Density Maps"
9:20 pm - 9:30 pm Discussion
TUESDAY
7:30 am - 8:30 amBreakfast
9:00 am - 12:30 pm3D Structure Analysis of Ordered Assemblies
9:00 am - 9:20 am Discussion Leader: Ingeborg Schmidt-Krey (Georgia Institute of Technology, Atlanta GA)
"Introduction and Overview"
9:20 am - 9:45 am Yoshinori Fujiyoshi (Kyoto University, Kyoto, Japan)
"Strong Points of Electron Crystallography Shown in Water and Ion Channels"
9:45 am - 10:00 am Discussion
10:00 am - 10:30 am Coffee Break
10:30 am - 10:55 am Tamir Gonen (University of Washington, Seattle, WA)
"Fragment Based Phase Extension in Electron Crystallography"
10:55 am - 11:10 am Discussion
11:10 am - 11:35 am Keiichi Namba (Osaka University, Osaka, Japan)
"High-resolution High-throughput Helical Image Analysis of Macromolecular Assemblies by Cryo-EM with In-Column Energy Filter and CCD"
11:35 am - 11:50 am Discussion
11:50 am - 12:15 pm Phoebe Stewart (Vanderbilt University, Nashville, TN)
"CryoEM Visualization and Modeling of Inserted Epitopes and Bound Peptides on the Adenovirus Capsid"
12:15 pm - 12:30 pm Discussion
12:30 pmLunch
1:30 pm - 4:00 pmFree Time
4:00 pm - 6:00 pmPoster Session
6:00 pmDinner
7:30 pm - 9:30 pmPoster Presentations and Discussion I
Discussion Leader: Eva Nogales (University of California, Berkeley, CA)
TBA (8 speakers, 10 min presentation and 5 min discussion each)
WEDNESDAY
7:30 am - 8:30 amBreakfast
9:00 am - 12:30 pm3D Reconstruction of Single Particles, Methods and Results II
9:00 am - 9:20 am Discussion Leader: Andres Leschziner (Harvard University, Cambridge, MA)
"Introduction and Overview"
9:20 am - 9:45 am Pawel Penczek (The University of Texas - Houston Medical School, Houston, TX)
"Stability and Reproducibility: an Objective Approach to 2D Alignment and Clustering"
9:45 am - 10:00 am Discussion
10:00 am - 10:30 am Coffee Break
10:30 am - 10:55 am Thomas Marlovits (Institute of Molecular Biotechnology, Austrian Academy of Sciences, Vienna, Austria)
"Dissecting the Injectisome for Sub-nanometer Resolution Structure Determination"
10:55 am - 11:10 am Discussion
11:10 am - 11:35 am Robert Langlois (Columbia University, New York, NY)
"Comparing the performance of semi-automated particle picking algorithms -- a clarification of machine-learning terms"
11:35 am - 11:50 am Discussion
11:50 am - 12:15 pm Helen Saibil (Birkbeck College, London, UK)
"Trajectory of Chaperonin Machine Movements Reconstructed from a Single Particle Data Set"
12:15 pm - 12:30 pm Discussion
12:30 pmLunch
1:30 pm - 4:00 pmFree Time
4:00 pm - 6:00 pmPoster Session
6:00 pmDinner
7:00 pm - 7:30 pmBusiness Meeting
Nominations for the next Vice Chair; Fill out Conference Evaluation Forms; Discuss future Site & Scheduling preferences; Election of the next Vice Chair
7:30 pm - 9:30 pmNew Results with Recent Techniques
7:30 pm - 7:45 pm Discussion Leader: Anchi Cheng (The Scripps Research Institute, La Jolla, CA)
"Introduction and Overview"
7:45 pm - 8:10 pm Yannik Schwab (IGBMC, Illkirch, France)
"Correlative Light and Electron Microscopy: From Live Imaging to 3D Ultrastructure"
8:10 pm - 8:20 pm Discussion
8:20 pm - 8:45 pm Wah Chiu (Baylor College of Medicine, Houston, TX)
"Zernike Phase Contrast Cryo-EM Reveals Previously Unseen Structural Features in Nanomachines"
8:45 pm - 8:55 pm Discussion
8:55 pm - 9:20 pm Daniel Wolf (Technical University Dresden, Dresden, Germany)
"Holographic Electron Tomography: 3D Imaging Using Direct Phase Contrast"
9:20 pm - 9:30 pm Discussion
THURSDAY
7:30 am - 8:30 amBreakfast
9:00 am - 12:30 pmElectron Tomography
9:00 am - 9:20 am Discussion Leader: Esther Bullit (Boston University School of Medicine, Boston, MA)
"Introduction and Overview"
9:20 am - 9:45 am Scott Stagg (Florida State University, Tallahassee, FL)
"Dealing with Heterogeneity in the COPII Coat"
9:45 am - 10:00 am Discussion
10:00 am - 10:30 am Coffee Break
10:30 am - 10:55 am Salvatore Lanzavecchia (Università degli Studi di Milano, Milan, Italy)
"3D Alignment and Averaging of Quasi Equivalent Volumes Extracted from Tomograms"
10:55 am - 11:10 am Discussion
11:10 am - 11:35 am Elisabeth Villa (Max-Planck-Institute for Biochemistry, Martinsried, Germany)
"Revealing the Molecular Architecture of the Nuclear Pore Complex using Cryo-Electron Tomography"
11:35 am - 11:50 am Discussion
11:50 am - 12:15 pm Daniela Nicastro (Brandeis University, Waltham, MA)
"Deconstructing Cilia and Flagella Function with Cryo-Electron Tomography and Structural Proteomics"
12:15 pm - 12:30 pm Discussion
12:30 pmLunch
1:30 pm - 4:00 pmFree Time
4:00 pm - 6:00 pmPoster Session
6:00 pmDinner
7:30 pm - 9:30 pmPoster Presentations and Discussion II
Discussion Leader: Montserrat Samsó (Virginia Commonwealth University, Richmond, VA)
TBA (8 speakers, 10 min presentation and 5 min discussion each)
FRIDAY
7:30 am - 8:30 amBreakfast
9:00 amDeparture
Funding for this conference was made possible (in part) by 5R13 GM085826-04 from the National Institutes of General Medicine Sciences. The views expressed in written conference materials or publications and by speakers and moderators do not necessarily reflect the official policies of the Department of Health and Human Services; nor does mention of trade names, commercial practices, or organizations imply endorsement by the U.S. Government.
 
© 2013 Gordon Research Conferences. All Rights Reserved. Search | Contact | Terms of Use | Privacy Policy | Follow us: Facebook Twitter