The cytoskeleton is a system of dynamic filaments used by both prokaryotic and eukaryotic cells to generate polarity, spatially organize cell contents, and generate forces underlying cell morphogenesis, movement, and division. The Gordon Research Conference on the Plant and Microbial Cytoskeleton focuses on the most exciting advances in cytoskeletal biology in plants, fungi, bacteria, and other microbes. Due to their unique biology, these organisms often use their cytoskeletons differently from animal cells, for example to construct and shape cell walls. Similarities and differences between these diverse organisms will stimulate new ideas and help to generate new insights into more general themes and concepts. Topics will include the regulation of cytoskeletal network organization and dynamics, and the role of the cytoskeleton in cell morphogenesis, cell division, intracellular motility, cell polarity, and cell wall formation. This meeting brings together researchers who use a variety of approaches, including molecular genetics, genomics, advanced cell imaging, biochemistry, in vitro reconstitution, ultrastructural analysis, and quantitative modeling. Scientists at all career stages will have opportunities present their work. The program includes approximately 30 invited speakers, 15 short talks selected from submitted abstracts, and multiple poster sessions. The meeting encourages participation of all attendees in discussions, and ensures ample opportunity for interactions among participants, fostering brainstorming and new collaborations across disciplines.