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Conference Program
 
Single Molecule Approaches to Biology
Understanding Life at a Higher Resolution
July 15-20, 2012
Mount Snow Resort
West Dover, VT

This conference aims to provide an interdisciplinary forum for biologists, physicists, chemists and engineers who have developed single molecule methodologies that can be readily applied to solve important biological problems. This emerging field of science attracts a young cohort of scientists who get a chance to interact closely with established leaders, promoting a creative environment of inquiry that greatly advances the field.

Early efforts of the single molecule community were almost entirely focused on developing new techniques. Now, and in large part due to this conference, single molecule techniques are rapidly occupying a central role in biological research at all levels. This transition was made possible by the availability and dissemination of robust techniques that use fluorescence and force probes to track the conformation of molecules one at a time, in vitro as well as in live cells. Single-molecule approaches have changed the way many biological problems are studied. These novel techniques provide previously unobtainable data on fundamental biochemical processes that are essential for all forms of life. The ability of single-molecule approaches to avoid ensemble averaging and to capture transient intermediates and heterogeneous behavior renders them particularly powerful in elucidating mechanisms of the molecular systems that underpin the functioning of living cells. Hence, our conference seeks to disseminate the implementation and use of single molecule techniques in the pursuit of new biological knowledge. In addition, the conference provides for a modern training ground for young scientists that wish to learn how to conduct interdisciplinary research.


Contributors

SUNDAY
2:00 pm - 9:00 pmArrival and Check-in (Office Closed 6:00 pm - 7:00 pm)
6:00 pmDinner
7:30 pm - 7:40 pmWelcome / Introductory Comments by GRC Site Staff
7:40 pm - 9:30 pmMolecular Motors on the Move
Discussion Leader: Stefan Hell (MPI, Gottingen)
7:40 pm - 8:20 pmKeynote presentation: Martin Karplus (Harvard University)
"Synergy between Single Molecule Simulations and Single Molecule Experiments-Applications to Biomolecular Motors"
8:20 pm - 8:35 pmDiscussion
8:35 pm - 9:15 pmYale Goldman (University of Pennsylvania)
"Molecular Motors: Adding Complexity One Molecule at a Time"
9:15 pm - 9:30 pmDiscussion
MONDAY
7:30 am - 8:30 amBreakfast
9:00 am - 12:30 pmOrigin And Fate Of Proteins
Discussion Leader: Xiaowei Zhuang (Harvard)
9:00 am - 9:35 amRobert Sauer (MIT)
"Exploration of the molecular mechanism of an ATP-fueled protein unfolding machine"
9:35 am - 9:45 amDiscussion
9:45 amGroup Photo / Coffee Break
10:15 am - 10:50 amRuben Gonzalez (Columbia University)
"Dynamics of a processive Brownian machine: the translating ribosome"
10:50 am - 11:00 amDiscussion
11:00 am - 11:35 amDavid Rueda (Wayne State University)
"Folding RNAs, big and small, one at a time"
11:35 am - 11:45 amDiscussion
11:45 am - 12:20 pmHelmut Grubmuller (MPI, Gottingen)
"Quantitative Single Molecule FRET Efficiency"
12:20 pm - 12:30 pmDiscussion
12:30 pmLunch
1:30 pm - 4:00 pmFree Time
4:00 pm - 6:00 pmPoster Session I
6:00 pmDinner
7:30 pm - 9:30 pmPhysical Principles Of Life
Discussion Leader: Shimon Weiss (UCLA)
7:30 pm - 7:50 pmYasmine Meroz (Weizmann Institute)
"Discerning Between Subdiffusive Models from Single Trajectories"
7:50 pm - 8:00 pmDiscussion
8:00 pm - 8:35 pmNynke Dekker (TU Delft)
"Measurement of Twist and Torque and Applications to Duplex RNA and Protein Binding"
8:35 pm - 8:45 pmDiscussion
8:45 pm - 9:20 pmSunney Xie (Harvard University)
"Life at the single molecule level"
9:20 pm - 9:30 pmDiscussion
TUESDAY
7:30 am - 8:30 amBreakfast
9:00 am - 12:30 pmMolecules and Super-resolution Microscopy
Discussion Leader: Taekjip Ha (University of Illinois)
9:00 am - 9:35 amJörg Wrachtrup (University of Stuttgart)
"Single defect sensors: Towards MRI on single cells and molecules"
9:35 am - 9:45 amDiscussion
9:45 amCoffee Break
10:15 am - 10:50 amSilvio Rizzoli (European Neuroscience Institute, Göttingen)
"Protein clusters: a comparison of antibody and small molecule labeling in high-resolution microscopy"
10:50 am - 11:00 amDiscussion
11:00 am - 11:35 amXiaowei Zhuang (Harvard University)
"Super-resolution fluorescence imaging with STORM"
11:35 am - 11:45 amDiscussion
11:45 am - 12:20 pmHolger Stark (MPI, Göttingen)
"Cryo-EM based Visualization of Dynamic Macromolecules: The Ribosome in Motion"
12:20 pm - 12:30 pmDiscussion
12:30 pmLunch
1:30 pm - 4:00 pmFree Time
4:00 pm - 6:00 pmPoster Session I
6:00 pmDinner
7:30 pm - 9:30 pmNanoswitches In Action
Discussion Leader: Timothy Springer (Harvard University)
7:30 pm - 7:50 pmIlaria Testa (MPI Göttingen)
"RESOLFT nanoscopy of living cells and tissues"
7:50 pm - 8:00 pmDiscussion
8:00 pm - 8:35 pmJohan Elf (University of Uppsala)
"Single molecule approaches to intracellular regulation"
8:35 pm - 8:45 pmDiscussion
8:45 pm - 9:20 pmSteve Block (Stanford University)
"Riboswitches and Gene Regulation Studied by Force Spectroscopy"
9:20 pm - 9:30 pmDiscussion
WEDNESDAY
7:30 am - 8:30 amBreakfast
9:00 am - 12:30 pmActive Motion Or Random Diffusion?
Discussion Leader: Julio Fernandez (Columbia University)
9:00 am - 9:40 amKeynote Presentation: Toshio Yanagida (Osaka University)
"Single molecules in vitro and vivo"
9:40 am - 10:00 amDiscussion
10:00 amCoffee Break
10:30 am - 11:10 amTom Kirchhausen (Harvard Medical School)
"Single-molecule biology and visualization of endocytosis"
11:10 am - 11:30 amDiscussion
11:30 am - 12:10 pmEric Greene (Columbia University)
"Visual Biochemistry: Single Molecule Imaging of Protein-DNA Interactions"
12:10 pm - 12:30 pmDiscussion
12:30 pmLunch
1:30 pm - 4:00 pmFree Time
4:00 pm - 6:00 pmPoster Session II
6:00 pmDinner
7:30 pm - 9:30 pmBuilding Blocks Of Living Cells
Discussion Leader: Michelle Wang (Cornell University)
7:30 pm - 7:50 pmTBA (Short Talk)
7:50 pm - 8:00 pmDiscussion
8:00 pm - 8:35 pmJeff Gelles (Brandeis University)
"Elucidating the mechanism of pre-messenger RNA splicing by chemical biology and CoSMoS"
8:35 pm - 8:45 pmDiscussion
8:45 pm - 9:20 pmViola Vogel (ETH Zürich)
"How cells exploit mechanical aspects of single molecules"
9:20 pm - 9:30 pmDiscussion
THURSDAY
7:30 am - 8:30 amBreakfast
8:30 am - 9:00 amBusiness Meeting
Nominations for the next Vice Chair; Fill out Conference Evaluation Forms; Discuss future Site & Scheduling preferences; Election of the next Vice Chair
9:00 am - 12:30 pmFrom Molecular Mechanics To Physiology
Discussion Leader: Hermann Gaub (LMU, Munich)
9:00 am - 9:35 amTimothy Springer (Harvard University)
"Single molecule studies on the arterial shear sensor protein von Willebrand factor"
9:35 am - 9:45 amDiscussion
9:45 amCoffee Break
10:15 am - 10:50 amOlga Dudko (UC San Diego)
"Theory and interpretation of single molecule force experiments"
10:50 am - 11:00 amDiscussion
11:00 am - 11:35 amLatha Venkataraman (Columbia University)
"Measuring Bond Rupture Forces at the Single-Molecule Level"
11:35 am - 11:45 amDiscussion
11:45 am - 12:20 pmCarlos Bustamante (UC Berkeley)
"Discrete steps by a DNA packaging motor and the inter-subunit coordination in a ring-ATPase"
12:20 pm - 12:30 pmDiscussion
12:30 pmLunch
1:30 pm - 4:00 pmFree Time
4:00 pm - 6:00 pmPoster Session II
6:00 pmDinner
7:30 pm - 9:30 pmTug-of-war: Force Spectroscopy Of Single Proteins
Discussion Leader: Kevin Plaxco (UCLA)
7:30 pm - 7:50 pmTBA (Short Talk)
7:50 pm - 8:00 pmDiscussion
8:00 pm - 8:35 pmSergi Garcia-Manyes (Kings College, London)
"Conformational dynamics of single proteins under force"
8:35 pm - 8:45 pmDiscussion
8:45 pm - 9:20 pmMatthias Rief (TU, Munich)
"Conformational dynamics of single protein molecules under load"
9:20 pm - 9:30 pmDiscussion
FRIDAY
7:30 am - 8:30 amBreakfast
9:00 amDeparture

Funding for this conference was made possible (in part) by 1 R13 GM 101703 - 01 from the National Institute of General Medical Sciences (NIGMS). The views expressed in written conference materials or publications and by speakers and moderators do not necessarily reflect the official policies of the Department of Health and Human Services; nor does mention by trade names, commercial practices, or organizations imply endorsement by the U.S. Government.

 
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