Protein science has undergone a revolution in the last decade. The elucidation of molecular structures at high resolution has become routine, vast amounts of genomic information now inform our analyses of protein structure, function and biology, and cutting edge technologies provide stunning glimpses into details of mechanism at ever higher resolution and shorter timescale. The 2013 Proteins Gordon Research Conference will bring together experimental and computational biochemists, biophysicists, chemists, biologists and engineers who study diverse aspects of proteins science, including protein structure, function, dynamics, thermodynamics, and evolution. The meeting will include sessions focused on the mechanical properties of proteins, which can be probed with exquisite single-molecule techniques, and the origins of stability, which can be examined using high-throughput random mutagenesis and deep sequencing. The controversial roles of water and cavities in defining the sensitivity of proteins to pressure will be discussed. There will also be talks about intricate molecular machines, and sessions that focus on large assemblies, membrane proteins, and the evolution of proteins and their interactions. Invited speakers will highlight a wide range of approaches for addressing fundamental and emerging problems in protein science. Speaking slots have also been reserved so that late-breaking results can be presented in short talks, and poster sessions will provide an opportunity for broad dissemination of participants’ research. In the relaxed, beautiful setting of Holderness, New Hampshire, ideas will flow between sub-disciplines and generations during collegial formal discussions, informal discussions, poster sessions, social events and afternoon recreational activities. Exciting recent advances in protein science and the strong historical tradition of this meeting promise an outstanding conference experience for participants at all career stages.