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Three Dimensional Electron Microscopy
Gordon Research Conference

Technical Advances for a Rising Star in Structural Biology

Dates

June 22-27, 2014

Location

PGA Catalunya Business and Convention Centre
Girona, Spain

Organizers

Chair:
Henning Stahlberg

Vice Chair:
Eva Nogales

Meeting Description

The 2014 Gordon Research Conference on Three-Dimensional Electron Microscopy (3DEM) will be the 20th meeting of a series that began in 1985. This conference will present leading method development and biological research in three-dimensional structure determination of biological samples, from macromolecules to cells. The meeting will highlight recent progress in sample preparation, imaging, and data analysis. Progress in cellular and molecular electron microscopy will be presented, covering electron tomography, sub-volume analysis, single particle cryo-EM, and electron microscopy of ordered assemblies from helical, spherical or planar 2D crystal arrangements, among others. 3DEM has become a key method in structural biology and cell biology. Recent breakthrough developments in detector technology significantly improve signal-to-noise ratios of recorded images or movies. Improved data analysis algorithms allow dealing with smaller or heterogeneous particles, reaching vastly improved resolution. 3DEM is a unique technique for the analysis of structure and conformational dynamics of a wide variety of specimens ranging from macromolecules to macromolecular assemblies to viruses, organelles and whole cells in their native environment. A focus at the conference will be on technological advances in instrumentation, software development, data processing, and tools for interpretation and validation. Progress in correlative imaging methods, as well as new alternative approaches will be presented. Finally, this conference will demonstrate examples how 3DEM studies help elucidate biological function.

The Conference will bring together the leading experts, junior researchers, and representatives of industrial partners. Following the tradition of previous conferences the program will offer an attractive combination of lectures and poster sessions. There will be two sessions with poster presenters selected for short talks. The program will also provide excellent opportunities for discussions and informal gatherings. Due to limited attendance (<200 participants) and the over-subscribed nature of previous meetings, early registration is encouraged.

Contributors

Meeting Program

SUNDAY
4:00 pm - 8:00 pmArrival and Check-in
6:00 pmDinner
7:30 pm - 7:40 pmWelcome / Introductory Comments by GRC Site Staff
7:40 pm - 9:30 pmKeynote Session: History and Frontiers in 3DEM
7:40 pm - 7:50 pmDiscussion Leader: Henning Stahlberg (University of Basel, CH)
"Conference Opening"
7:50 pm - 8:30 pmMichael Rossmann (Purdue University, IN, USA)
"3DEM in Life Sciences: A Personal History of Structural Biology and the Growth of Electron Microscopy"
8:30 pm - 8:40 pmDiscussion
8:40 pm - 9:20 pmPaul Midgley (University of Cambridge, UK)
"3DEM in Materials Sciences: Recent Advances in 3D Materials Characterization by Electron Microscopy"
9:20 pm - 9:30 pmDiscussion
MONDAY
7:30 am - 8:30 amBreakfast
9:00 am - 12:30 pmSample Preparation for Cryo-EM
9:00 am - 9:10 amDiscussion Leader: David Stokes (New York University, NY, USA)
"Introduction and Overview"
9:10 am - 9:30 amRouslan Efremov (University of Brussels, BE)
"Structure and Dynamics of the Ryanodine Channel Reconstituted into Lipid Nanodiscs"
9:30 am - 9:45 amDiscussion
9:45 am - 10:05 amChristopher Russo (MRC Laboratory of Molecular Biology, Cambridge, UK)
"Controlling Protein Adsorption on Graphene for Electron Cryo-Microscopy"
10:05 am - 10:20 amDiscussion
10:20 amCoffee Break
10:50 am - 11:10 amIngeborg Schmidt-Krey (Georgia Institute of Technology, GA, USA)
"2D Crystal Handling"
11:10 am - 11:20 amDiscussion
11:20 am - 11:40 amJürgen Plitzko (MPI for Biochemistry, Martinsried, Germany, and Utrecht University, NL)
"Magic Milling - FIB Specimen Preparation for Cryo-Electron Tomography"
11:40 am - 11:55 amDiscussion
11:55 am - 12:15 pmThomas Braun (University of Basel, Switzerland)
"Single Cell Visual Proteomics"
12:15 pm - 12:30 pmDiscussion
12:30 pmLunch
1:30 pm - 4:30 pmFree Time
4:30 pm - 6:00 pmPoster Session A
6:00 pm - 8:00 pmProgress in Molecular and Cellular EM Through Instrumentation Advances
6:00 pm - 6:15 pmDiscussion Leader: Werner Kühlbrandt (MPI for Biophysics, Frankfurt, DE)
"Introduction and Overview"
6:15 pm - 6:35 pmHolger Stark (MPI for Biophysical Chemistry, Göttingen, DE)
"Dissecting Limitations in High Resolution cryo-EM"
6:35 pm - 6:50 pmDiscussion
6:50 pm - 7:10 pmWei Dai (Baylor College of Medicine, Houston, TX, USA)
"Visualizing Virus Assembly Intermediates Inside Cyanobacteria by Zernike Phase Contrast cryoET"
7:10 pm - 7:25 pmDiscussion
7:25 pm - 7:45 pmRadostin Danev (MPI for Biochemistry, Martinsried, DE)
"A Phase Plate for Automated Cryo-Tomography and Single-Particle Analysis"
7:45 pm - 8:00 pmDiscussion
8:00 pmDinner
TUESDAY
7:30 am - 8:30 amBreakfast
8:30 amGroup Photo
9:00 am - 12:30 pmValidation and Data Exchange
9:00 am - 9:15 amDiscussion Leader: Richard Henderson (MRC, Cambridge, UK)
"Introduction and Overview"
9:15 am - 9:30 amArdan Patwardan (EBI, Hinxton, UK)
"EMDB: Validation, Raw Data and Cellular EM"
9:30 am - 9:40 amDiscussion
9:40 am - 10:00 amNiels Volkmann (Sanford-Burnham Medical Research Institute, La Jolla, CA, USA)
"Statistics-Based Evaluation and Validation Approaches for Fitting of Atomic Models into Electron Microscopy Reconstructions"
10:00 am - 10:10 amDiscussion
10:10 amCoffee Break
10:40 am - 11:00 amGunnar Schroeder (Forschungszentrum Juelich, Juelich, Germany)
"Validation of Atomic Models Obtained from Cryo-EM Data"
11:00 am - 11:10 amDiscussion
11:10 am - 11:30 amPawel Penczek (University of Texas, TX, USA)
"EM Map Validation Through Convergence Consistency"
11:30 am - 11:45 amDiscussion
11:45 am - 12:15 pmSriram Subramaniam (NCI, NIH, Bethesda, MD, USA)
"The Slippery Road from Purified Protein to Validated 3D Structure"
12:15 pm - 12:30 pmDiscussion
12:30 pmLunch
1:30 pm - 4:30 pmFree Time
4:30 pm - 6:00 pmPoster Session A
6:00 pm - 8:00 pmSelected Poster Presentations
Discussion Leader: Eva Nogales (UC Berkeley, CA, USA)
"Introduction and Overview"
6:00 pm - 6:10 pmXiaochen Bai (MRC-LMB)
"Three-Dimensional Structure of Human Gamma-Secretase"
6:10 pm - 6:15 pmDiscussion
6:15 pm - 6:25 pmBettina Boettcher (University of Edinburgh)
"Hepatitis B Core Pre-Mature Envelopment Mutant at 4.5 Å Resolution"
6:25 pm - 6:30 pmDiscussion
6:30 pm - 6:40 pmSandra Braukman (University Mainz)
"4 Å Structure and Ligand Binding of the Dodecahedral Acetylcholine-Binding Protein from the Snail Biomphalaria glabrata"
6:40 pm - 6:45 pmDiscussion
6:45 pm - 6:55 pmFrank Dimaio (University of Washington)
"Automitic Structure Determination, Local Rebiulding and Refinement into Sub-5 Å Density Maps"
6:55 pm - 7:00 pmDiscussion
7:00 pm - 7:10 pmKay Gruenewald (University of Oxford)
"Integrative Imaging of Cellular Membrane Modulation by Herpesvirus"
7:10 pm - 7:15 pmDiscussion
7:15 pm - 7:25 pmAndy Hoenger (University of Colorado, Boulder)
"Microtubule Associated Protein in the Giardia Ventral Disc"
7:25 pm - 7:30 pmDiscussion
7:30 pm - 7:40 pmSebastian Kube (Ludwig-Maximilians University)
"Structure of VipA/B Type VI Secretion Complex Suggests a Contraction-State Specific Recycling Mechanism"
7:40 pm - 7:45 pmDiscussion
7:45 pm - 7:55 pmHarry Low (Birkbeck)
"Structure of the Bacterial Type IV Secretion System"
7:55 pm - 8:00 pmDiscussion
8:00 pmDinner
WEDNESDAY
7:30 am - 8:30 amBreakfast
9:00 am - 12:30 pmNew Approaches to Imaging
9:00 am - 9:10 amDiscussion Leader: Andreas Engel (Delft University of Technology, The Netherlands)
"Introduction and Overview"
9:10 am - 9:30 amJan Peter Abrahams (Leiden University, Leiden, NL)
"High Resolution Analysis of 100 nm Protein 3D Nano-Crystals by Electron Imaging and Diffraction"
9:30 am - 9:45 amDiscussion
9:45 am - 10:05 amTamir Gonen (Janelia Farm, VA, USA)
"Protein Structure Determination by MicroED"
10:05 am - 10:20 amDiscussion
10:20 amCoffee Break
10:50 am - 11:10 amJames Evans (Pacific Northwest National Lab, Richland, WA, USA)
"Imaging Protein Structure and Dynamics with Short Electron Pulses and Ultrafast X-Ray Free Electron Lasers"
11:10 am - 11:20 amDiscussion
11:20 am - 11:40 amSharon Wolf (Weizman Institute, Rehovot, Israel)
"Cryo-Scanning Transmission Electron Tomography of Vitrified Whole Cells"
11:40 am - 11:55 amDiscussion
11:55 am - 12:15 pmMaofu Liao (UCSF, San Francisco, CA, USA)
"Single-Particle Electron Cryo-Microscopy Reveals High-Resolution Structure and Activation Mechanisms of TRPV1 Ion Channel"
12:15 pm - 12:30 pmDiscussion
12:30 pmLunch
1:30 pm - 4:30 pmFree Time
4:30 pm - 6:00 pmPoster Session B
6:00 pm - 8:00 pmProgress in Image Processing
6:00 pm - 6:15 pmDiscussion Leader: Steve Ludtke (Baylor College of Medicine, Houston, TX, USA)
"Introduction and Overview"
6:15 pm - 6:35 pmCarsten Sachse (EMBL, Heidelberg, DE)
"Single-Particle Helical Processing - From Symmetry Determination to High-Resolution Structures"
6:35 pm - 6:50 pmDiscussion
6:50 pm - 7:10 pmHemant Tagare (Yale University, CT, USA)
"Fast Maximum-Likelihood Single Particle Reconstruction in Cryo-EM"
7:10 pm - 7:25 pmDiscussion
7:25 pm - 7:45 pmSjors Scheres (MRC, Cambridge, UK)
"How to Get More Out of Your Data with Statistical Image Processing"
7:45 pm - 8:00 pmDiscussion
8:00 pmDinner
THURSDAY
7:30 am - 8:30 amBreakfast
8:30 am - 9:00 amBusiness Meeting
Nominations for the Next Vice Chair; Fill in Conference Evaluation Forms; Discuss Future Site and Scheduling Preferences; Election of the Next Vice Chair
9:00 am - 12:30 pmCryo-EM at High Resolution
9:00 am - 9:10 amDiscussion Leader: Stefan Raunser (MPI of Molecular Physiology, Dortmund, DE)
"Introduction and Overview"
9:10 am - 9:30 amJanet Vonck (MPI for Biophysics, Frankfurt, DE)
"Protein Complex Structures Using a Direct Electron Detector"
9:30 am - 9:40 amDiscussion
9:40 am - 10:00 amJoachim Frank (Columbia University, New York, NY, USA)
"Electron Microscopy and Ab Initio Modeling of Ribosomes - A Dream Come True"
10:00 am - 10:10 amDiscussion
10:10 amCoffee Break
10:40 am - 11:00 amJohn Briggs (EMBL, Heidelberg, DE)
"Applications of Sub-Tomogram Averaging at Sub-nm Resolution"
11:00 am - 11:10 amDiscussion
11:10 am - 11:30 amDorit Hanein (Sanford-Burnham Medical Research Institute, La Jolla, CA, USA)
"Correlating Light and Cryo-ET of the Actin Cytoskeleton in Cellular Environment"
11:30 am - 11:40 amDiscussion
11:40 am - 12:15 pmKeynote Lecture: Grant Jensen (Caltech, Pasadena, CA, USA)
"Correlated Cryo-PALM/Electron Cryo Tomography and Type VI Secretion"
12:15 pm - 12:30 pmDiscussion
12:30 pmLunch
1:30 pm - 4:30 pmFree Time
4:30 pm - 6:00 pmPoster Session
6:00 pm - 8:00 pmSelected Poster Presentations (continued)
Discussion Leader: Eva Nogales (UC Berkeley, CA, USA)
"Introduction and Overview"
6:00 pm - 6:10 pmMisha Kudryashev (University of Basel)
"Sub-Tomogram Average with Dynamo"
6:10 pm - 6:15 pmDiscussion
6:15 pm - 6:25 pmArne Moeller (Aaurhus University)
"Engineered Nanostructrued Beta-Sheet Peptides Protect Membrane Proteins"
6:25 pm - 6:30 pmDiscussion
6:30 pm - 6:40 pmPeter Rosenthal (MRC)
"Specimen Charging and Its Mitigation"
6:40 pm - 6:45 pmDiscussion
6:45 pm - 6:55 pmJeffrey Speir (Scripps)
"Typhon and Spototon: Multilayeres TEM Sample Preparation"
6:55 pm - 7:00 pmDiscussion
7:00 pm - 7:10 pmJulian von der Ecken (MPI)
"F-Actin at Atomistic Resolution"
7:10 pm - 7:15 pmDiscussion
7:15 pm - 7:25 pmPing Zhu (CHinese Academy of Sciences)
"Cryo-EM Study of the Chromatin 30-nm Fiber"
7:25 pm - 7:30 pmDiscussion
7:30 pm - 7:40 pmZeev-Ben-Mordehai Tzviya (Oxford)
"Membrane Protein Enriched Extracellular Vesicles (MPEEVs): A Novel Platform for the Structure Determination of Membrane Proteins by CryoET"
7:40 pm - 7:45 pmDiscussion
7:45 pm - 7:55 pmBenoit Zuber (University of Bern)
"A New Tool Facilitating Vitreous Cryo-Sectioning (CEMOVIS)"
7:55 pm - 8:00 pmDiscussion
8:00 pmDinner
FRIDAY
7:30 am - 8:30 amBreakfast
9:00 amDeparture
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