|2:00 pm - 9:00 pm||Arrival and check-in |
| Session 1.||New Technologies for Studies of Second Messenger Signaling|
|Discussion Leaders: E. Degerman (Univ. of Lund) & S. Francis (Vanderbilt University)|
|7:30-8:00 ||Opening Remarks|
|8:00-8:30 || Natalie Ahn University of Colorado at Boulder, Boulder, CO|
Analysis of regulated protein dynamics by deuterium exchange mass spectrometry
|8:30-9:00 || Jeffrey Karpen University of Colorado School of Medicine, Denver, CO|
Resolution of cAMP signals in three-dimensional microdomains using novel, real-time sensors.
|9:00-9:30|| Wolfgang Dostmann University of Vermont, Burlington, VT|
Cygnets: the dynamics of intracellular cGMP revealed by genetically encoded fluorescent indicators
|9:45-10:45 pm||Welcome Reception|
| Session 2.||Cyclic Nucleotides & Regulation of Energy Metabolism|
| Discussion Leaders: J. Corbin (Vanderbilt University) & M. Houslay (Univ. of Glasgow)|
|9:00-9:30 || Sven Enerbäck Goteborg University, Goteborg, SWEDEN |
FOXC2 is a winged helix gene that counteracts obesity, hypertriglyceridemia and diet induced insulin resistance
|9:30-10:00 || Vince Manganiello National Heart and Lung Institute, Bethesda, MD.|
Characterization of PDE3B KO mice.
|10:00-10:30 || Hideichi Makino Ehime University School of Medicine, Ehime, Japan|
Identification of a PDE3B interacting protein using the yeast two-hybrid system
|11:00-11:30 || Tamar Michaeli Albert Einstein School of Medicine, New York, NY|
PDEs and regulation of insulin secretion and action
|11:30-12:00|| Allen Zhao University of Pittsburgh School of Medicine, Pittsburgh, PA|
Leptin, PDE3B, and regulation of body weight.
|12:00-12:15|| Eva Degerman (Short Talk) University of Lund, Lund, SWEDEN|
Role of PDE3B in the regulation of energy metabolism.
|12:15-12:30|| Hanguan Liu (Short talk) National Heart and Lung Institute, Bethesda, MD|
Characterization of the PDE3B promotor.
|1:30 pm - 4:30 pm||Free Time|
|4:30 pm - 6:00 pm||Poster Session I|
| Session 3.||GAFs and Cyclic nucleotide-binding domains |
| Discussion Leaders: N. Artemyev (Univ. of Iowa) & R. Cote (Univ. of New Hampshire)|
| 7:30-8:00 || L. Aravind National Center for Biotechnology Information, NIH, Bethesda, MD|
Evolutionary history and diversity of cNMP cyclases, phosphodiesterases and associated small molecule binding domains.
| 8:00-8:30|| Joe Beavo University of Washington, Seatttle, WA|
PDE2 GAF/cGMP-binding site structure: How good a model for other GAF domains?
| 8:30-9:00 || Joachim Schultz University of Tuebingen. Tubingen, Germany|
A GAF-domain operated cAMP switch: adenylyl cyclase from Anabaena
| 9:00-9:30 || Rick Cote University of New Hampshire, Durham NH|
Allosteric interactions between catalytic and inhibitory subunits of PDE6 mediated by its GAF domains.
|9:30-9:45 || Gary Kruh (Short talk) Fox Chase Cancer Center, Philadelphia, PA|
Cyclic nucleotide efflux pumps
| Session 4.||Cyclic Nucleotides & PDEs: Proliferation, Apoptosis, & Differentiation|
| Discussion Leaders: M. Conti (Stanford University) & C. Lugnier (University of Strasbourg)|
|9:00-9:30|| Leonard Forte University of Missouri School of Medicine, Columbia MO
Gene expression in colon tumors: guanylin and uroguanylin, receptor-guanylate cyclase and cyclic GMP signaling molecules.
|9:30-10:00|| Joe Thompson Cell Pathways, Inc., Horsham, PA|
SAANDS induce apoptosis via cGMP-mediated mechanisms.
|10:00-10:30 || Tom Lincoln University of Alabama at Birmingham, Birmingham, AL|
Expression of PKG-I in smooth muscle cells: implications in vascular disease.
|11:00-11:30 || Claire Lugnier University of Strasbourg, Illkirch, FRANCE|
PDEs as potential targets in angiogenesis.
|11:30-11:45 || Dietrich Dettmer (Short talk) University of Leipzig, Leipzig, GERMANY|
Behavior of PDE2 and PDE3 during transformation of rat hepatic stellate cells to myofibroblast-like cells
|11:45-12:00 || Douglas Tilley (short talk) Queen's University, Kingston, ON, Canada|
"Vascular smooth muscle PDE3 and PDE4 activities and levels are regulated by cAMP levels."
|12:00-12:15 ||Hermann Tenor (short talk) Byk Gulden, Konstanz, Germany|
" PDE isoenzyme families in osteoarthritis chondrocytes-functional importance of PDE4."
|12:15-12:30 ||Adam Lerner (short talk) Boston Medical Center, Boston, MA|
Caspase 9 plays a key role in rolipram-induced apoptosis in primary chronic lymphocytic leukemia cells
|1:30 pm - 4:30 pm||Free Time|
|4:30 pm - 6:00 pm||Poster Session I (continued) |
| Session 5.||Selective Expression of PDEs: Regulation & Function|
| Discussion Leaders: C. Jin (Stanford University) & D. Dettmer (Univ. of Leipzig)|
|7:30-8:00 || Marco Conti , Stanford University Medical Center, Stanford, CA |
Structure and function of different PDE4 isoforms.
| 8:00-8:30 ||Don Maurice, Queens University at Kingston, Kingston, Ontario, CANADA|
The impact of vascular smooth muscle cell phenotype on phosphodiesterase expression: Impact on regulated cell function.
| 8:30-9:00 || Carolyn Smith , DesignWrite, Princeton, NJ|
Regulation of cAMP PDE3 and cGMP PDE5 genes in cardiovascular injury (heart failure and hypertension).
| 9:00-9:30|| Ching Lin , University of California, San Francisco|
Human PDE5A gene expression and regulation.
|9:30-9:45|| Chen Yan (Short talk) University of Rochester, Rochester, NY|
Regulation of PDE1A gene expression in vascular smooth muscle cells.
| Session 6. ||Compartmentalization of Cyclic Nucleotides & Their Intracellular Targets|
| Discussion Leaders: D. Maurice (Queen's University) & C. Smith (DesignWrite)|
| 9:00-9:30 || Laurence Brunton University of California-San Diego, La Jolla CA|
Compartmentation of cyclic nucleotide signaling in cardiac cells
| 9:30-10:00|| Rodolphe Fischmeister Universite de Paris-Sud, Paris. France|
Contribution of different PDE subtypes to cAMP compartmentation in cardiac myocytes
| 10:00-10:15|| Manuela Zaccolo (Short Talk) University of Padua, Padua, ITALY|
cAMP microdomains in heart cells.
|10:45-11:15 || Miles Houslay University of Glasgow, Glasgow, SCOTLAND|
Intracellular targeting of PDE4 isoforms
|11:15-11:45 ||Kjetil Tasken University of Oslo, Oslo, NORWAY |
PKA-PDE signalling units
| 11:45-12:00 ||Jun Kotera (short talk) Tanabe Seiyaku Company, Toda, Saitama, JAPAN|
Subcellular localization of phosphodiesterase 10A variants
|12:00-12:15||Matthew Movsesian (short talk) University of Utah, Salt Lake City, UT|
Intracellular compartmentation of PDE3 activity and effects of cAMP and
cGMP hydrolysis in cardiac and vascular myocytes
|12:15-12:30||Wilma van Staveren Maastricht University, Maastricht,
Characterization of the cellular localization of cGMP-hydrolyzing PDEs in the rat hippocampus and related structures
|1:30 pm - 4:30 pm||Free Time|
|4:30 pm - 6:00 pm||Poster Session II |
|Session 7.||Pharmacological Targeting of Cyclic Nucleotide Signaling Pathways|
| Discussion Leaders: M. Barnette (GlaxoSmithKline) & R. Colman (Temple Univ.)|
| 7:30-8:00||Natalie Mount Pfizer Global Research and Development, Sandwich, ENGLAND|
New therapeutic opportunities for PDE5 inhibitors.
| 8:00-8:30 || Jun-ichi Kambayashi Otsuka Maryland Research Institute, Rockville, MD|
Current progress in clinical and pre-clinical research on cilostazol
|8:30-9:00|| Erwin Bischoff Bayer-AG|
Physiological effects of pharmacologic cGMP elevation.
|9:00-9:30 || Zheng Huang Merck Frosst, Pointe-Claire-Dorval, CANADA|
Mechanistic insights into PDE4 catalyzed cAMP hydrolysis and implications to inhibitor design.
|9:30-9:45||Peter Schafer (short talk) Celgene Corporation, Warren, NJ|
CC-110004: a novel PDE4 inhibitor with an improved therapeutic index in a lung neutrophilia model
|Session 8.||Insights into Structure & Function of PDEs|
| Discussion Leaders: J. Beavo (University of Washington) & J. Thompson (Cell Pathways, Inc.)|
|9:00-9:30 || Hengming Ke University of North Carolina at Chapel Hill, Chapel Hill, NC|
Crystal structure of the PDE4-rolipramcomplex provides insight into the binding and the inhibitor selectivity.
|9:30-10:00|| Robert Colman Temple University School of Medicine, Philadelphia, PA |
PDE3A: Active and inhibitory sites as revealed by mutagenesis, affinity labeling, and molecular modeling.
|10:00-10:30|| Nikolai Artemyev University of Iowa School of Medicine, Iowa City IA|
Structural determinants of PDE6 function
|10:30-11:00 || Break|
|11:00-11:30|| Ted Wensel Baylor College of Medicine, Houston, TX|
Regulation of PDE6 in rod and cone photoreceptors.
|11:30-12:00 || Jackie Corbin/Sharron Francis Vanderbilt University School of Medicine, Nashville, TN|
Allosteric and catalytic sites of PDE5
|12:00-12:15 || Kenji Omori (Short talk) Tanabe Seiyaku Company, Toda, Saitama, JAPAN|
Comparison of characteristics of PDE8 family
|12:15-12:30||Wito Richter (short talk) Stanford University, Stanford, CA|
Involvement of upstream conserved regions in the dimerization of the
|1:30 pm - 4:30 pm||Free Time|
|4:30 pm - 6:00 pm||Poster Session II (continued) |
| Session 9. ||Selected PDEs: Specificity in Catalysis, Regulation & Function|
| Discussion Leaders: V. Manganiello (National Institutes of Health) & T. Michaeli (Albert Einstein)|
|7:30-8:00 || Business Session|
| 8:00-8:30|| Akio Yamazaki Wayne State University School of Medicine, Detroit, MI|
Turnoff of GTP-activated PDE6 withouth GTP hydrolysis: role of cyclin-dependent protein kinase 5.
|8:30-9:00||Doris Koesling Ruhr-Universitat Bochum, Bochum, GERMANY|
A new regulatory mechanism within NO/cGMP signalling
|9:00-9:15|| Karnam Murthy (Short Talk) Medical College of Virginia, Richmond, VA|
Regulation of PDE5 and soluble guanylate cyclase by cGMP-dependent
protein kinase in smooth muscle
|9:15-9:30 ||Kelley Bentley Univ. of Michigan, Ann Arbor, MI|
Increases in PDE2 phosphorylation by an associated NGF-stimulated kinase
|7:30 am - 8:30 am||Breakfast|
|9:00 am ||Departure|