SUNDAY, JUNE 22
Session 1: (Sunday p.m.)
Topic: Spectroscopic Approaches to Membrane Protein-Ligand Interactions
Discussion Leader: Brian K. Kobilka, Stanford University
- 7:30 pm - Welcoming Remarks from the Chair
- 7:35 pm - Presentation of the Alexander M. Cruickshank Lecture Award to Wayne L. Hubbell
- 7:40-8:10 pm - Wayne L Hubbell, Jules Stein Eye Institute, Los Angeles, CA, - AIexander M Cruickshank Lecture Award - "Exploring protein structure and dynamics with site-directed spin labeling"
- 8:10-8:20 pm - Discussion
- 8:20-8:45 pm - André Chollet, Geneva Biomedical Research Institute, Geneva, Switzerland - "Probing the structure, function and dynamics of transmembrane proteins through biosynthetic incorporation of fluorescent amino acids"
- 8:45-8:55 pm - Discussion
- 8:55-9:20 pm - Brian K Kobilka, Stanford University Medical Center, Stanford, CA - "Spectroscopic approaches to studying helix interactions in adrenergic receptors"
- 9:20-9:30 pm - Discussion
MONDAY, JUNE 23
Session 2: (Monday a.m.)
Topic: Physical Analysis of Membrane Protein-Ligand Structure
Discussion Leader: Steven O. Smith, Yale University, New Haven, CT
- 9:00-9:10 am - Introduction by Steven Smith
- 9:10-9:40 am - Joseph J. Falke, University of Colorado, Boulder, CA - "The aspartate receptor: mechanism of ligand-induced transmembrane signaling"
- 9:40-9:55 - Discussion
- 9:55-10:15 am - Group Picture and Coffee Break
- 10:15-10:40 am - Steven Smith, Yale University - "Functional role of glycine residues in receptor tyrosine kinase and G protein-coupled receptor activation"
- 10:40-10:50 am - Discussion
- 10:50-11:00 am - Lynmarie Thompson, University of MA, Amherst - "Local structure and structural changes in bacterial chemotaxis receptors"
- 11:00-11:30 am - Tracy M. Handel, University of California, Berkeley - "Structural and functional studies of chemokine receptor ligands"
- 11:30-11:45 am - Discussion
- 11:45-12:15 am - Timothy A. Cross, Florida State University, Tallahassee, FL - "Solid State NMR studies of the gramicidin A channel: a model for cation selectivity"
- 12:15-12:30 am - Discussion
5-6 pm - Poster Session I
Session 3: (Monday p.m.)
Topic: Onwards to Three Dimensional Structures of lon Channels
Discussion Leader: Robert H. Guy, National Cancer Institute
- 7:00-7:15 pm - Introduction by Robert Guy
- 7:15-7:45 pm - Jorg Rosenbusch, University of Basel, Switzerland - "Crystallization of membrane proteins: a rational approach in three dimensions"
- 7:45-8:00 pm - Discussion
- 8:00-8:30 pm - Dennis A. Dougherty, CalTech, Pasadena, CA - "High precision studies of the nicotinic receptor using unnatural amino acid mutagenesis"
- 8:30-8:45 - Discussion
- 8:45-9:15 pm - Robert H. Guy, National Cancer Institute, Bethesda, MD - "Computational models of the structure and gating mechanisms of K+ channels"
- 9:15-9:30 pm - Discussion
TUESDAY, JUNE 24
Session 4: (Tuesday a.m.)
Topic: Molecular Determinants of lon Channel Gating
Discussion Leader: William A. Catterall, University of Washington, Seattle, WA
- 9:00-9:30 am - Arthur Karlin, Columbia University, New York, NY - "The structures of the acetylcholine receptor channel in the open and closed states"
- 9:30-9:40 am - Discussion
- 9:40-9:50 am - Peter Schofield, Garvan Institute, Sydney - "Structural, functional, and genetic insights into ligand binding and signal transduction at the inhibitory glycine receptor"
- 9:50-10:20 am - Steven A. Siegelbaum, Ctr Neurobiology & Behavior, Columbia University - "Molecular mechanism of cyclic nucleotide-gated ion channel activation"
- 10:20-10:30 am - Discussion
- 10:30-10:55 am - Coffee Break
- 10:55-11:25 am - Diane M. Papazian, UCLA School of Medicine, Los Angeles, CA - "Structural interactions in the voltage sensor of Shaker K+ channels"
- 11:25-11:35 am - Discussion
- 11:35-11:45 pm - Marco Columbini, University of MD - "Localization and motion of the voltage sensor of the mitochondrial channel VDAC
- 11:45-12:15 pm - William Catterall, University of Washington - "Molecular Interactions in Sodium Channel Inactivation Gating."
- 12:15-12:30 pm - Discussion
5-6 pm - Poster Session II
Session 5: (Tuesday p.m.)
Topic: Transporter-Ligand Interactions: Biophysical Approaches
Discussion Leader: Diane Papazian, University of California @ Los Angeles, Los Angeles, CA
- 7:30-7:45 pm - Introduction by Diane Papazian
- 7:45-8:15 pm - H. Ronald Kaback, University of California @ Los Angeles, Los Angeles, CA - "The Lac permease of E. coli: if 1 Cys mutant is good, 400 are better"
- 8:15-8:30 pm - Discussion
- 8:30-9:00 pm - Michael P. Kavanaugh, Vollum Institute, Portland, OR - "Glutamate transporters: ion recognition of gates & pores"
- 9:00-9:30 pm - Discussion
WEDNESDAY, JUNE 25
Session 6: (Wednesday a.m.)
Topic: Transporter-Ligand Interactions: Structure/Function
Discussion Leader: Robert Edwards, University of California @ San Francisco, San Francisco, CA
- 9:00-9:10 am - Introduction by Robert Edwards
- 9:10-9:40 am - T. Hastings Wilson, Harvard, Boston, MA - "Alteration of Na-coupled transport in site-directed mutants of the melibiose carrier"
- 9:40-9:50 am - Discussion
- 9:50-10:20 am - Jonathan Javitch, Columbia University, NYC, NY - "Structure/activity studies of dopamine D2 receptor and dopamine transporter"
- 10:20-10:30 am - Discussion
- 10:30-10:45 am - Coffee Break
- 10:45-11:15 am - Ron R. Kopito, Stanford University, Palo Alto, CA - "Ligand or substrate? Gating of CFTR Cl Channels by ATP"
- 11:15-11:25 am - Discussion
- 11:25-11:55 am - Robert Edwards, University of California @ San Francisco, San Francisco, CA - "Substrate recognition by vesicular neurotransmitter transporters and its relationship to drug inhibition and bioenergetic coupling"
- 11:55-12:05 pm- Discussion
- 12:05-12:20 pm - Additional Discussants
- 12:20-12:30 pm - Conclusions by Robert Edwards
Session 7: (Wednesday p.m.)
Topic: Helix-Helix and Helix-Membrane Interactions
Discussion Leader: Jürgen Wess, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, MD
- 7:30-7:40 pm - Introduction by Jürgen Wess
- 7:40-8:20 pm - Kevin MacKenzie, Yale University, New Haven, CT - "Helix interactions in membrane proteins and oligomers"
- 8:20-8:30 pm - Discussion
- 8:30-9:10 pm - Dan Oprian, Brandeis University, Waltham, MA - "State-dependent disulfide bond formation in rhodopsin"
- 9:10-9:20 pm - Discussion
- 9:20-9:25 pm - Mark Krebs, University of Wisconsin - "Thermodynamic analysis of bacteriorhodopsin self-assembly in halobacterium salinarium"
- 9:25-9:30 pm - Discussion
THURSDAY, JUNE 26
Session 8. (Thursday a.m.)
9:00-9:20 am - Business Meeting
Topic: Mechanisms of 7TM Receptor Activation and Inactivation
Discussion Leader: Kathryn Sandberg, Georgetown University, Washington, DC
- 9:20-9:40 am - Introduction by Kathryn Sandberg
- 9:40-10:10 am - Janos Lanyi, University of California @ Irvine, Irvine, CA - "Conformational shuttling and proton translocation in bacteriorhodopsin"
- 10:10-10:20 am - Discussion
- 10:20-10:30 am - Paula Booth, Imperial College, London - "Controlling membrane protein folding"
- 10:30-10:50 am - Coffee Break
- 10:50-11:20 am - Marvin Gershengorn, Cornell University, New York, NY - "Agonist binding and interhelical interactions in TRH receptor activation"
- 11:20-11:30 am - Discussion
- 11:30-11:40 am - Robert Leduc, University of Sherbrooke, Canada - "Mapping of the angiotensin II contact points in the AT1 and AT2 receptors with photoaffinity labeling analogs"
- 11:40-12:10 pm - Thue W. Schwartz Rigshospitalet, Copenhagen, Denmark - "Metal-ion sites as functional and structural probes in 7TM receptors"
- 12:10-12:20 pm - Discussion
- 12:20-12:30 pm - Robert C. Speth, Washington State University - "TOPO: A tool for two-dimensional representation of membrane spanning proteins"
6:00 pm - GALA Dinner and Awards Ceremony
Session 9. (Thursday p.m.)
Topic: Constitutively Active Transmembrane Proteins
Discussion Leader: Susanna Cotecchia, University of Lausanne, Lausanne, Switzerland
- 7:30-7:40 pm - Introduction by Susanna Cotecchia
- 7:40-8:05 pm - Jean-Claude Bonnafous, CNRS, Montpellier, France - "Constitutive activation of angiotensin II receptors upon amino acid mutation in the third transmembrane domain; progress towards the modeling of signal transduction processes"
- 8:05-8:15 pm - Discussion
- 9:15-8:40 pm - James Konopka, State University of New York, Stony Brook, NY - "Mutations in transmembrane domain Vl that constitutively activate the alpha-factor pheromone receptor in yeast"
- 8:40-8:50 pm - Discussion
- 8:50-9:25 pm - Laurent Schild, University of Lausanne, Lausanne, Switzerland - "Gain of function mutations in the epithelial sodium channel causing hypertension"
- 9:25-9:35 pm - Discussion