The 2022 Gordon Research Conference on Notch Signaling in Development, Regeneration and Disease will provide a stimulating, international and cross disciplinary forum for the dissemination and discussion of new research, concepts, and therapeutic opportunities at the forefront of Notch related biology. The Notch signaling pathway is a central mediator of short-range inter-cellular communication in multicellular organisms, and alterations of Notch activity underlie or contribute to a growing list of developmental syndromes, adult onset diseases and cancers, expanding the interest in this pathway well beyond the boundaries of basic developmental and cell biology and into the areas of stem cell biology, cancer biology and therapeutics. The meeting will bring together a diverse community of scientists from academia, biotech and the pharmaceutical industry working in nearly every model organism and organ system, creating a stimulating scientific environment in a relaxed setting where cutting edge technologies, ideas and discoveries can be exchanged and where students and research fellows will gain exposure to leaders in the field. Time will also be set aside for short talks by selected poster presenters. The informal and confidential environment in GRC meetings encourages free scientific exchange of unpublished data. The inclusion and integration of a Gordon Research Seminar (GRS) exclusively for postdocs and graduate students prior to the GRC is a particular asset to draw new scientists into the field and encourage trainee participation. Most importantly, the combined GRC/GRS format excels in integrating students, postdocs and investigators wishing to enter a new field, such as the study of this important signaling pathway.