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Three Dimensional Electron Microscopy
Gordon Research Conference

Advancing and Reshaping Structural Biology with Electron Cryo-Microscopy

Dates

June 19-24, 2016

Location

The Chinese University of Hong Kong
Hong Kong, China

Organizers

Chair:
Yifan Cheng

Vice Chair:
John Briggs

Hong Kong Advisory Board:
Tony F. Chan, Kathryn Song Eng Cheah, Fanny M.C. Cheung, Arthur B. Ellis, Barry Halliwell, Nancy Ip, Jian Lu, Peter Mathieson, Alfonso Ngan, Paul Tam, Benjamin Wah, Henry Wong, Vivian Wing-Wah Yam, Kenneth Young & Albert Cheung-Hoi Yu More Information

Meeting Description

The 2016 Gordon Research Conference on Three-Dimensional Electron Microscopy (3DEM) will continue its 30+ years tradition of highlighting recent advances in both technological developments and their applications in addressing critical biological questions. For the first time, the 3DEM conference will be held in Asia.

3DEM is a versatile method in structural and cell biology. In recent years, the field of 3DEM has experienced and is still experiencing a resolution revolution brought by major technological breakthroughs. Challenging targets and questions in structural biology that have been intractable for many years, ranging from membrane proteins to large and dynamic machineries and assemblies, are becoming feasible targets of single particle cryo-EM. Electron tomography and sub-volume averaging are also producing detailed structural information of assemblies in the cellular context with unprecedented resolutions. The extraordinary excitements and opportunities thus attract many scientists, structural biologists in particular, from outside of 3DEM field to not only learn and practice the state-of-the-art cryo-EM techniques for their favorite targets, but also bring their own expertise and ideas to improve the techniques. The rapid technological developments and advancement coupled with the rapid expansion of the field brought tremendous opportunities as well as serious challenges to 3DEM. The landscapes of both 3DEM as well as structural biology in general are experiencing significant changes. Thus, the theme of the 2016 GRC on 3DEM is Reshaping Structural Biology.

The 2016 conference will continue its tradition to discuss the latest technological advents, progress in applying the methods to addressing important and critical biological questions, methodologies and criteria in validating critical results, as well as data deposition and accessibility. It will also provide a forum for thorough discussions among both established experts and junior scientists from different disciplines, with either academia or industrial backgrounds. Following the tradition of 3DEM GRC meetings, the 2016 meeting encourages participants to present recent and unpublished scientific results, in both oral and poster presentations. Two evening sessions are dedicated for selected poster presentations. Again, due to limited attendance (<200 participants) and the over-subscribed nature of previous meetings, particularly the 2015 meeting, early registration is encouraged.

Contributors

Final Meeting Program

Sunday
2:00 pm - 8:00 pmArrival and Check-in
6:00 pmDinner
7:30 pm - 7:40 pmWelcome / Introductory Comments by GRC Site Staff
7:40 pm - 9:30 pmKeynote Session: Reshaping Structural Biology with Cryo-EM
Discussion Leader: Yifan Cheng (University of California, San Francisco, USA)
7:40 pm - 7:50 pmOpening Remarks
7:50 pm - 8:25 pmStephen Harrison (Harvard Medical School, USA)
"Lessons from X-Ray Crystallography for the Transition from Methodology to Structural Biology in CryoEM"
8:25 pm - 8:40 pmDiscussion
8:40 pm - 9:15 pmRichard Henderson (MRC Laboratory of Molecular Biology, United Kingdom)
"Good, Better and Best in CryoEM"
9:15 pm - 9:30 pmDiscussion
Monday
7:30 am - 8:30 amBreakfast
9:00 am - 12:30 pmMethodologies that Drive the Field Forward
Discussion Leader: Keiichi Namba (Graduate School of Frontier Biosciences, Osaka University, Japan)
9:00 am - 9:20 amIntroduction by Discussion Leader
9:20 am - 9:45 amJoachim Frank (Columbia University, USA)
"Milestones in the History of Single Particle Cryo-EM"
9:45 am - 10:00 amDiscussion
10:00 am - 10:25 amDavid Agard (Howard Hughes Medical Institute / University of California, San Francisco, USA)
"New Advances in Motion Correction Extend Obtainable Resolutions at Both 200kV and 300kV"
10:25 am - 10:40 amDiscussion
10:40 am - 11:10 amGroup Photo / Coffee Break
11:10 am - 11:35 amSjors Scheres (Medical Research Council, United Kingdom)
"New Developments in RELION"
11:35 am - 11:50 amDiscussion
11:50 am - 12:15 pmMarcus Brubaker (University of Toronto, Canada)
"Algorithms for Reducing the Computational Burden of CryoEM"
12:15 pm - 12:30 pmDiscussion
12:30 pmLunch
1:30 pm - 4:00 pmFree Time
3:00 pm - 4:00 pmPower Hour
The GRC Power Hour is an optional informal gathering open to all meeting participants. It is designed to help address the challenges women face in science and support the professional growth of women in our communities by providing an open forum for discussion and mentoring.
Organizers: Eva Nogales (University of California, Berkeley, USA) and Bridget Carragher (New York Structural Biology Center, USA)
4:00 pm - 6:00 pmPoster Session
6:00 pmDinner
7:30 pm - 9:30 pmValidation of Cryo-EM Structures, from Maps to Atomic Models
Discussion Leader: John Rubinstein (University of California San Francisco, USA)
7:30 pm - 7:45 pmIntroduction by Discussion Leader
7:45 pm - 8:05 pmIrina Serysheva (University of Texas Medical School at Houston, USA)
"Single-Particle Cryo-EM of IP3R Calcium Channel: Structural Validation"
8:05 pm - 8:20 pmDiscussion
8:20 pm - 8:40 pmJiawei Wang (School of Life Sciences, Tsinghua University, China)
"Combination of X-Ray Crystallographic and Single-Particle Cryo-EM Methods"
8:40 pm - 8:55 pmDiscussion
8:55 pm - 9:15 pmThomas Marlovits (Austrian Academy of Sciences, Austria)
"Unbiased Functional Validation - From Structures to Systems"
9:15 pm - 9:30 pmDiscussion
Tuesday
7:30 am - 8:30 amBreakfast
9:00 am - 12:30 pmStructures that Are Beyond the Reach of X-Ray Crystallography
Discussion Leader: Georgios Skiniotis (University of Michigan, USA)
9:00 am - 9:20 amIntroduction by Discussion Leader
9:20 am - 9:45 amEva Nogales (University of California, Berkeley, USA)
"Structural Transitions During Eukaryotic Transcription Initiation Visualized by Cryo-EM"
9:45 am - 10:00 amDiscussion
10:00 am - 10:25 amYigong Shi (Tsinghua University, China)
"Cryo-EM Structures of the Spliceosome and the U4/U6.U5 tri-snRNP: Insights into Pre-mRNA Splicing"
10:25 am - 10:40 amDiscussion
10:40 am - 11:10 amCoffee Break
11:10 am - 11:35 amThi Hoang Duong Nguyen (University of California, Berkeley, USA)
"Cryo-EM Structure of the Spliceosomal U4/U6.U5 tri-snRNP and Dealing with Its Conformational Heterogeneity"
11:35 am - 11:50 amDiscussion
11:50 am - 12:15 pmHuilin Li (Stony Brook University, USA)
"'On-Grid Biochemistry' and the Eukaryotic DNA Replication"
12:15 pm - 12:30 pmDiscussion
12:30 pmLunch
2:00 pm - 4:00 pmPoster Session
4:00 pm - 6:00 pmSelected Poster Presentations: Recent Advances in Cryo-EM
Discussion Leader: Robert Glaeser (Lawrence Berkeley National Laboratory, USA)
4:00 pm - 4:10 pmAnchi Cheng (New York Structural Biology Center, USA)
"Life on the Terrace with an Image Shift View"
4:10 pm - 4:15 pmDiscussion
4:15 pm - 4:25 pmNadav Elad (Weizmann Institute of Science, Israel)
"Detection of Isolated Metal Atoms on Protein by Cryo-Scanning Transmission Electron Microscopy"
4:25 pm - 4:30 pmDiscussion
4:30 pm - 4:40 pmYoshiyuki Fukuda (Max Planck Institute of Biochemistry, Germany)
"In Situ Studies of Tripeptidyl Peptidase II: Structure and Spatial Coordination with 26S Proteasome"
4:40 pm - 4:45 pmDiscussion
4:45 pm - 4:55 pmChristos Gatsogiannis (Max Planck Institute of Molecular Physiology, Germany)
"Syringe-Like Injection Mechanism of Bacterial Tc Toxins Revealed in Molecular Detail by CryoEM"
4:55 pm - 5:00 pmDiscussion
5:00 pm - 5:10 pmEmma Hesketh (University of Leeds, United Kingdom)
"Mechanisms of Assembly and Genome Packaging in an RNA Virus Revealed by High-Resolution Cryo-EM"
5:10 pm - 5:15 pmDiscussion
5:15 pm - 5:25 pmIoan Iacovache (University of Bern, Switzerland)
"Cryo-EM Structure of Aerolysin Variants Reveals a Novel Protein Fold and the Pore Formation Process"
5:25 pm - 5:30 pmDiscussion
5:30 pm - 5:40 pmGabriel Lander (The Scripps Research Institute, USA)
"Why One Atomic Model? A Rationale for Atomic Ensembles"
5:40 pm - 5:45 pmDiscussion
5:45 pm - 5:55 pmSharon Wolf (Weizmann Institute of Science, Israel)
"CryoSTEM Tomography of Mitochondria In Situ in Unsectioned Whole Vitrified Cells"
5:55 pm - 6:00 pmDiscussion
6:00 pmDinner
Wednesday
7:30 am - 8:30 amBreakfast
9:00 am - 12:30 pmStructures in the Cellular Context
Discussion Leader: Karen Davies (Lawrence Berkeley National Laboratory, USA)
9:00 am - 9:20 amIntroduction by Discussion Leader
9:20 am - 9:45 amJohn Briggs (European Molecular Biology Laboratory, Germany)
"Approaches to Subtomogram Averaging at Higher-Resolutions"
9:45 am - 10:00 amDiscussion
10:00 am - 10:25 amElizabeth Villa (University of California, San Diego, USA)
"Opening Windows into the Nucleus: Bringing Structure to Cell Biophysics"
10:25 am - 10:40 amDiscussion
10:40 am - 11:10 amCoffee Break
11:10 am - 11:35 amHuy Bui (McGill University, Canada)
"In Situ Structural Analysis of the Human Nuclear Pore Complex by Cryo-ET"
11:35 am - 11:50 amDiscussion
11:50 am - 12:15 pmWei Dai (Rutgers University, USA)
"Visualizing Biological Processes with CryoET"
12:15 pm - 12:30 pmDiscussion
12:30 pmLunch
1:30 pm - 4:00 pmFree Time
4:00 pm - 6:00 pmPoster Session
6:00 pmDinner
7:00 pm - 7:30 pmBusiness Meeting
Nominations for the Next Vice Chair; Fill in Conference Evaluation Forms; Discuss Future Site and Scheduling Preferences; Election of the Next Vice Chair
7:30 pm - 9:30 pmStrategies for Improving Cryo-EM Sample Preparation and Quality
Discussion Leader: Lori Passmore (MRC Laboratory of Molecular Biology, United Kingdom)
7:30 pm - 7:45 pmIntroduction by Discussion Leader
7:45 pm - 8:05 pmBridget Carragher (New York Structural Biology Center, USA)
"A New Approach to Preparing TEM Grids"
8:05 pm - 8:20 pmDiscussion
8:20 pm - 8:40 pmLiguo Wang (University of Washington, USA)
"Random Spherically Constrained (RSC) Single-Particle Cryo-EM for Structures of Membrane Proteins in a Membrane Environment"
8:40 pm - 8:55 pmDiscussion
8:55 pm - 9:15 pmYuan Gao (University of California, San Francisco, USA)
"TRPV1 Ion Channel in Lipid - Mechanism of Lipid and Ligand Action"
9:15 pm - 9:30 pmDiscussion
Thursday
7:30 am - 8:30 amBreakfast
9:00 am - 12:30 pmEmerging New Cryo-EM Technologies
Discussion Leader: Peijun Zhang (University of Pittsburgh, USA)
9:00 am - 9:20 amIntroduction by Discussion Leader
9:20 am - 9:45 amYoshinori Fujiyoshi (CeSPI, Nagoya University, Japan)
"Issues to Be Considered for Image Based Structure Analysis"
9:45 am - 10:00 amDiscussion
10:00 am - 10:25 amTamir Gonen (Howard Hughes Medical Institute, USA)
"Progress in MicroED - Three Dimensional Electron Diffraction of Microscopic Crystals"
10:25 am - 10:40 amDiscussion
10:40 am - 11:10 amCoffee Break
11:10 am - 11:35 amWerner Kuehlbrandt (Max Planck Institute of Biophysics, Germany)
"Structure and Mechanism of Mitochondrial ATP Synthase Determined by Single-Particle Cryo-EM and Electron Cryo-Tomography"
11:35 am - 11:50 amDiscussion
11:50 am - 12:15 pmRadostin Danev (Max Planck Institute of Biochemistry, Germany)
"Single Particle Analysis with the Volta Phase Plate"
12:15 pm - 12:30 pmDiscussion
12:30 pmLunch
1:30 pm - 4:00 pmFree Time
4:00 pm - 6:00 pmSelected Poster Presentations: Recent Advances in the Application of Cryo-EM Methodology
Discussion Leader: Sharon Wolf (Weizmann Institute of Science, Israel)
4:00 pm - 4:10 pmAndreas Boland (MRC Laboratory of Molecular Biology, United Kingdom)
"The Cryo-EM Structure of a Separase-Securin Complex at Near Atomic Resolution"
4:10 pm - 4:15 pmDiscussion
4:15 pm - 4:25 pmDmitry Lyumkis (The Salk Institute for Biological Studies, USA)
"Single-Particle Cryo-EM of Preferred Orientation Samples with Tilts"
4:25 pm - 4:30 pmDiscussion
4:30 pm - 4:40 pmDoreen Matthies (National Institutes of Health, USA)
"Single-Particle Cryo-EM Studies of a 200 kDa Magnesium Ion Channel Reveal Large Structural Changes upon Gating"
4:40 pm - 4:45 pmDiscussion
4:45 pm - 4:55 pmPeter Peters (Maastricht Multimodal Molecular Imaging Institute (M4I), Maastricht University, The Netherlands)
"A Novel Fully Automated Method for Vitrifying Biological Samples on Pre-Mounted Autogrids"
4:55 pm - 5:00 pmDiscussion
5:00 pm - 5:10 pmMaryam Khoshouei (Max-Planck Institute of Biochemistry, Germany)
"Phase Contrast Single Particle Analysis at Near Atomic Resolutions"
5:10 pm - 5:15 pmDiscussion
5:15 pm - 5:25 pmSoung-Hun Roh (Baylor College of Medicine, USA)
"Detecting Conformational Variants in a Single GroEL by CryoEM"
5:25 pm - 5:30 pmDiscussion
5:30 pm - 5:40 pmRicardo Righetto (Biozentrum, University of Basel, Switzerland)
"Unveiling Conformational Heterogeneity in 2D Crystals with Single-Particle Refinements"
5:40 pm - 5:45 pmDiscussion
5:45 pm - 5:55 pmAli Dashti (University of Wisconsin-Milwaukee, USA)
"Energy Landscape and Continuous Conformational Movies of RYR1"
5:55 pm - 6:00 pmDiscussion
6:00 pmDinner
Friday
7:30 am - 8:30 amBreakfast
9:00 amDeparture

Any opinions, findings, conclusions or recommendations expressed in this material/event (or by members of the project team) do not reflect the views of the Government of the Hong Kong Special Administrative Region, the Innovation and Technology Commission or the Vetting Committee of the General Support Programme of the Innovation and Technology Fund.
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