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Single Molecule Approaches to Biology
Gordon Research Conference

Single-Molecule Microscopy: Life at a Higher Resolution

Dates

July 3-8, 2016

Location

The Chinese University of Hong Kong
Hong Kong, China

Organizers

Chairs:
David Rueda & Toshio Yanagida

Vice Chairs:
Julie Biteen & Antoine Van Oijen

Hong Kong Advisory Board:
Tony F. Chan, Kathryn Song Eng Cheah, Fanny M.C. Cheung, Arthur B. Ellis, Barry Halliwell, Nancy Ip, Jian Lu, Peter Mathieson, Alfonso Ngan, Paul Tam, Benjamin Wah, Henry Wong, Vivian Wing-Wah Yam, Kenneth Young & Albert Cheung-Hoi Yu More Information

Meeting Description

This conference aims to provide an interdisciplinary forum for biologists, physicists, chemists and engineers who develop single molecule methodologies that can be readily applied to solve important biological problems. This emerging field of science attracts a young cohort of scientists who get a chance to interact closely with established leaders, promoting a creative environment of inquiry that greatly advances the field.

Early efforts of the single molecule community were almost entirely focused on techniques development. Now, and in large part due to this conference, single molecule techniques are rapidly occupying a central role in biological research at all levels. This transition was made possible by the availability and dissemination of robust techniques that use fluorescence and force probes to track the conformation of molecules one at a time, in vitro as well as in live cells. Single-molecule approaches have changed the way many biological problems are studied. These novel techniques provide previously unobtainable data on fundamental biochemical processes that are essential for all forms of life. The ability of single-molecule approaches to avoid ensemble averaging and to capture transient intermediates and heterogeneous behavior renders them particularly powerful in elucidating mechanisms of the molecular systems that underpin the functioning of living cells. Hence, our conference seeks to disseminate the implementation and use of single molecule techniques in the pursuit of new biological knowledge. In addition, the conference provides for a modern training ground for young scientists that wish to learn how to conduct interdisciplinary research. To encourage this exchange and networking amongst the junior scientists a two day Gordon Research Seminar is held prior to the conference.

Related Meeting

This GRC was held in conjunction with the "Single Molecule Approaches to Biology" Gordon Research Seminar (GRS). Refer to the associated GRS program page for more information.

Contributors

Final Meeting Program

Sunday
2:00 pm - 8:00 pmArrival and Check-in
6:00 pmDinner
7:30 pm - 7:40 pmWelcome / Introductory Comments by GRC Site Staff
7:40 pm - 9:30 pmKeynote Session: Imaging Biology from Single Molecules to Single Cells
Discussion Leader: David Rueda (Imperial College London, United Kingdom)
7:40 pm - 8:20 pmTaekjip Ha (Johns Hopkins University, USA)
"Revisiting and Repurposing the Double Helix"
8:20 pm - 8:35 pmDiscussion
8:35 pm - 9:15 pmXiaowei Zhuang (Harvard University / Howard Hughes Medical Institute, USA)
"Illuminating Biology at the Nanoscale with Single-Molecule and Super-Resolution Imaging"
9:15 pm - 9:30 pmDiscussion
Monday
7:30 am - 8:30 amBreakfast
9:00 am - 12:30 pmNucleic Acids Enzymes
Discussion Leader: Antoine Van Oijen (University of Wollongong, Australia)
9:00 am - 9:10 amIntroduction by Discussion Leader
9:10 am - 9:40 amEric Greene (Columbia Univeristy, USA)
"Single Molecule Studies of Genetic Recombination"
9:40 am - 9:50 amDiscussion
9:50 am - 10:20 amGijs Wuite (VU University Amsterdam, The Netherlands)
"Sliding Sleeves of XRCC4-XLF Bridge DNA and Connect DNA-Fragments"
10:20 am - 10:30 amDiscussion
10:30 am - 11:00 amGroup Photo / Coffee Break
11:00 am - 11:30 amSungchul Hohng (Seoul National University, South Korea)
"Co-Transcriptional Folding of RNA Studied via Single-Molecule FRET"
11:30 am - 11:40 amDiscussion
11:40 am - 11:55 amHoura Merrikh (University of Washington, USA)
"Visualization of Replisome Dynamics In Vivo"
11:55 am - 12:00 pmDiscussion
12:00 pm - 12:15 pmChirlmin Joo (Delft University of Technology, The Netherlands)
"A Dynamic Search Process Underlies MicroRNA Targeting"
12:15 pm - 12:20 pmDiscussion
12:20 pm - 12:30 pmPoster Previews
12:30 pmLunch
1:30 pm - 4:00 pmFree Time
4:00 pm - 6:00 pmPoster Session
6:00 pmDinner
7:30 pm - 9:30 pmTransactions on Chromatin
Discussion Leader: Jan Liphardt (Stanford University, USA)
7:30 pm - 7:40 pmIntroduction by Discussion Leader
7:40 pm - 8:10 pmMaxime Dahan (Institut Curie, France)
"Search Dynamics of Individual Nuclear Factors"
8:10 pm - 8:20 pmDiscussion
8:20 pm - 8:35 pmMelike Lakadamyali (ICFO - The Institute of Photonic Sciences, Spain)
"Decoding Chromatin Organization with Super-Resolution Microscopy"
8:35 pm - 8:40 pmDiscussion
8:40 pm - 8:55 pmMichael Stone (University of California, Santa Cruz, USA)
"Structure and Dynamics of Single Telomerase Reverse Transcriptase Enzymes"
8:55 pm - 9:00 pmDiscussion
9:00 pm - 9:15 pmJie Xiao (Johns Hopkins School of Medicine, USA)
"Spatial and Functional Organization of Transcription in Bacterial Cells"
9:15 pm - 9:20 pmDiscussion
9:20 pm - 9:30 pmGeneral Discussion
Tuesday
7:30 am - 8:30 amBreakfast
9:00 am - 12:30 pmGene Expression and Processing
Discussion Leader: Achillefs Kapanidis (University of Oxford, United Kingdom)
9:00 am - 9:10 amIntroduction by Discussion Leader
9:10 am - 9:40 amMichelle Wang (Cornell University, USA)
"Single Molecule Force Spectroscopy"
9:40 am - 9:50 amDiscussion
9:50 am - 10:20 amJeff Gelles (Brandeis University, USA)
"Multi-Wavelength Studies of Transcription Regulation"
10:20 am - 10:30 amDiscussion
10:30 am - 11:00 amCoffee Break
11:00 am - 11:30 amMaria Carmo-Fonseca (University of Lisbon, Portugal)
"Live-Cell Imaging of Transcription and RNA Processing with Single Molecule Resolution"
11:30 am - 11:40 amDiscussion
11:40 am - 11:55 amDaniel Larson (National Cancer Insitute, NIH, USA)
"Coordinate Regulation of Estrogen Responsive Genes and Regulatory RNA in Living Cells"
11:55 am - 12:00 pmDiscussion
12:00 pm - 12:15 pmAaron Hoskins (University of Wisconsin-Madison, USA)
"Single Molecule Insights into the Mechanism and Regulation of Pre-Spliceosome Assembly"
12:15 pm - 12:20 pmDiscussion
12:20 pm - 12:30 pmPoster Previews
12:30 pmLunch
2:00 pm - 4:00 pmPoster Session
4:00 pm - 6:00 pmFolding and Function
Discussion Leader: Olga Dudko (University of California, San Diego, USA)
4:00 pm - 4:10 pmIntroduction by Discussion Leader
4:10 pm - 4:40 pmBenjamin Schuler (University of Zurich, Switzerland)
"Probing Intrinsically Disordered Proteins with Single-Molecule Spectroscopy"
4:40 pm - 4:50 pmDiscussion
4:50 pm - 5:05 pmMichael Woodside (University of Alberta, Canada)
"Measuring Transition Paths in the Folding of Single Molecules"
5:05 pm - 5:10 pmDiscussion
5:10 pm - 5:25 pmSergi Garcia-Manyes (King's College London, United Kingdom)
"Linking Mechanochemistry to Protein Folding at the Single Bond Level"
5:25 pm - 5:30 pmDiscussion
5:30 pm - 5:45 pmDavid Lilley (University of Dundee, United Kingdom)
"Studying and Exploiting Fluorophore Orientation in Single-Molecule FRET Experiments"
5:45 pm - 5:50 pmDiscussion
5:50 pm - 6:00 pmGeneral Discussion
6:00 pmDinner
Wednesday
7:30 am - 8:30 amBreakfast
9:00 am - 12:30 pmSuper-Resolution Imaging and Emerging Technologies
Discussion Leader: Julie Biteen (University of Michigan, USA)
9:00 am - 9:10 amIntroduction by Discussion Leader
9:10 am - 9:40 amPeng Yin (Harvard University, USA)
"DNA Based Nanoscopy"
9:40 am - 9:50 amDiscussion
9:50 am - 10:20 amTakeharu Nagai (Osaka University, Japan)
"A Spontaneously Switchable Fluorescent Protein for a PALM-Based Easy and Fast Super-Resolution Microscopy"
10:20 am - 10:30 amDiscussion
10:30 am - 11:00 amCoffee Break
11:00 am - 11:30 amJonas Korlach (Pacific Biosciences, USA)
"Single Molecule, Real-Time Biology in Zero-Mode Waveguides"
11:30 am - 11:40 amDiscussion
11:40 am - 11:55 amHiroyuki Noji (The University of Tokyo, Japan)
"Robustness of Catalysis, Allostery, and Torque-Transmission Mechanism of F1-ATPase"
11:55 am - 12:00 pmDiscussion
12:00 pm - 12:15 pmIbrahim Cisse (Massachusetts Institute of Technology, USA)
"Pol II Cluster Dynamics Predict mRNA Output In Vivo"
12:15 pm - 12:20 pmDiscussion
12:20 pm - 12:30 pmPoster Previews
12:30 pmLunch
1:30 pm - 4:00 pmFree Time
4:00 pm - 6:00 pmPoster Session
6:00 pmDinner
7:30 pm - 9:30 pmNeuronal Biophysics
Discussion Leader: Yasushi Okada (Quantitative Biology Center, RIKEN, Japan)
7:30 pm - 7:40 pmIntroduction by Discussion Leader
7:40 pm - 8:10 pmSubhojit Roy (University of California, San Diego, USA)
"Know Your Trail: The Hitchhiker's Guide to Axonal Actin"
8:10 pm - 8:20 pmDiscussion
8:20 pm - 8:35 pmAntonie Triller (École Normale Supérieure / INSERM, France)
"The Synapse Stability and Plasticity: A Single Molecule Approach to Chemistry In Cellulo"
8:35 pm - 8:40 pmDiscussion
8:40 pm - 8:55 pmRoland Brandt (University of Osnabrück, Germany)
"Exploring Cytoskeletal and RNA-Binding Protein Dynamics in Living Neuronal Cells by Single Molecule Tracking"
8:55 pm - 9:00 pmDiscussion
9:00 pm - 9:15 pmDimitrios Stamou (University of Copenhagen, Denmark)
"A New Technique Able to Record Transporter Activity at the Single-Molecule Level"
9:15 pm - 9:20 pmDiscussion
9:20 pm - 9:30 pmGeneral Discussion
Thursday
7:30 am - 8:30 amBreakfast
8:30 am - 9:00 amBusiness Meeting
Nominations for the Next Vice Chair; Fill in Conference Evaluation Forms; Discuss Future Site and Scheduling Preferences; Election of the Next Vice Chair
9:00 am - 12:30 pmMechanobiology
Discussion Leader: Thomas Perkins (JILA, University of Colorado Boulder, USA)
9:00 am - 9:10 amIntroduction by Discussion Leader
9:10 am - 9:40 amHermann Gaub (Ludwig Maximilian University of Munich, Germany)
"Force Propagation in Biomolecular Complexes and Force Activated Kinases"
9:40 am - 9:50 amDiscussion
9:50 am - 10:20 amYale Goldman (University of Pennsylvania, USA)
"Rotational Motions and Fluctuations in Motors and the Ribosome"
10:20 am - 10:30 amDiscussion
10:30 am - 11:00 amCoffee Break
11:00 am - 11:30 amArne Gennerich (Albert Einstein College of Medicine, USA)
"Single-Molecule Studies of Cytoplasmic Dynein Motion and Force Generation"
11:30 am - 11:40 amDiscussion
11:40 am - 11:55 amTakayuki Uchihashi (Kanazawa University, Japan)
"Direct Visualization of Single Molecule Dynamics with High-Speed Atomic Force Microscopy"
11:55 am - 12:00 pmDiscussion
12:00 pm - 12:15 pmAlexander Dunn (Stanford University, USA)
"Molecular Mechanisms Underlying Force Transduction at Intercellular Adhesion Complexes"
12:15 pm - 12:20 pmDiscussion
12:20 pm - 12:30 pmPoster Previews
12:30 pmLunch
1:30 pm - 4:00 pmFree Time
4:00 pm - 6:00 pmCell Signaling
Discussion Leader: Mariko Okada (RIKEN, Japan)
4:00 pm - 4:10 pmIntroduction by Discussion Leader
4:10 pm - 4:40 pmMasahiro Ueda (Osaka University, Japan)
"Single Molecule Analysis of Intracellular Signaling in Eukaryotic Chemotaxis"
4:40 pm - 4:50 pmDiscussion
4:50 pm - 5:05 pmKatharina Gaus (Single Molecule Science, University of New South Wales, Australia)
"The T Cell Signalling Network - A Single Molecule Perspective"
5:05 pm - 5:10 pmDiscussion
5:10 pm - 5:25 pmTakahiro Fujiwara (Kyoto University, Japan)
"Mesoscopic Organization and Function of the Plasma Membrane: An Ultrafast Single-Molecule Imaging Study"
5:25 pm - 5:30 pmDiscussion
5:30 pm - 5:45 pmXavier Darzacq (University of California, Berkeley, USA)
"Single Molecule Imaging to Study Transcription Regulation"
5:45 pm - 5:50 pmDiscussion
5:50 pm - 6:00 pmGeneral Discussion
6:00 pmDinner
Friday
7:30 am - 8:30 amBreakfast
9:00 amDeparture

Any opinions, findings, conclusions or recommendations expressed in this material/event (or by members of the project team) do not reflect the views of the Government of the Hong Kong Special Administrative Region, the Innovation and Technology Commission or the Vetting Committee of the General Support Programme of the Innovation and Technology Fund.
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