Gordon Research Conferences
Meeting Details
Search:
  GO

Membrane Protein Folding
Gordon Research Conference

Folding Biophysics, Design, and Stabilization with Applications to Disease

Dates

June 4-9, 2017

Location

Stonehill College
Easton, MA

Organizers

Chair:
James U. Bowie

Vice Chair:
Charles R. Sanders

Meeting Description

An understanding of protein folding is essential for most of the major quests of structural biology, including learning how to predict structure, how proteins evolve, how mutations cause disease and how to design novel proteins. As a result, protein folding has been a major field of investigation for the past 50 years. In this effort, however, membrane proteins have been largely ignored. In-depth folding experiments on membrane proteins have lagged behind their soluble counterparts not because membrane proteins are unimportant, but because they are simply hard to study. This conference will bring together cellular, biophysical and computational investigations of membrane protein folding and design with the purpose of sharing expertise and solutions to this challenging problem.

Contributors

Meeting Program

Sunday
2:00 pm - 9:00 pmArrival and Check-in
6:00 pmDinner
7:30 pm - 7:40 pmWelcome / Introductory Comments by GRC Site Staff
7:40 pm - 9:30 pmMembrane Integration and Removal of Helical Membrane Proteins
Discussion Leader: James Bowie (University of California, Los Angeles, USA)
7:40 pm - 7:45 pmOpening Remarks
7:45 pm - 8:10 pmStephen White (University of California, Irvine, USA)
"Translocon Recognition and Membrane Stability of Transmembrane Helices"
8:10 pm - 8:20 pmDiscussion
8:20 pm - 8:45 pmJoachim Herberle (Freie Universitat Berlin, Germany)
"In-Situ Observation of Membrane Protein Insertion and Folding During Cell-Free Expression by SEIRAS"
8:45 pm - 8:55 pmDiscussion
8:55 pm - 9:20 pmRyan Baldridge (Harvard Medical School, USA)
"Mechanism of ER-Associated Protein Degradation (ERAD)"
9:20 pm - 9:30 pmDiscussion
Monday
7:30 am - 8:30 amBreakfast
8:30 amGroup Photo
9:00 am - 12:30 pmBiophysics of Helical Membrane Protein Folding
Discussion Leader: Charles Deber (University of Toronto, Canada)
9:00 am - 9:25 amPaula Booth (King's College London, United Kingdom)
"Folding Alpha Helical Membrane Proteins: Thermodynamics, Lipid Bilayer Mechanics and Co-Translational Folding"
9:25 am - 9:35 amDiscussion
9:35 am - 9:45 amDieter Langosch (Technical University of Munich, Germany)
"BLaTM, a Genetic Tool to Measure Homo- and Heterotypic Parallel and Antiparallel Transmembrane Helix-Helix Interactions"
9:45 am - 9:50 amDiscussion
9:50 am - 10:15 amTetiana Serdiuk (ETH Zurich, Switzerland)
"Observing the Insertase-Assisted Insertion and Folding Steps of Single Membrane Proteins"
10:15 am - 10:25 amDiscussion
10:25 am - 10:35 amDuyoung Min (University of California, Los Angeles, USA)
"Folding of ClC Chloride Transporter Retains Memory of Its Evolutionary History"
10:35 am - 10:40 amDiscussion
10:40 am - 11:05 amCoffee Break
11:05 am - 11:30 amThomas Perkins (JILA, University of Colorado Boulder / National Institute of Standards and Technology, USA)
"Hidden Dynamics in the Unfolding of Individual Bacteriorhodopsin Proteins"
11:30 am - 11:40 amDiscussion
11:40 am - 11:50 amAna Nicoleta Bondar (Freie Universitaet Berlin, Germany)
"Mechanism by Which Lipids Shape Reaction Coordinates of Rhomboid Proteases"
11:50 am - 11:55 amDiscussion
11:55 am - 12:20 pmJebrell Glover (Lehigh University, USA)
"Biophysical and Biochemical Insights into Caveolin-1"
12:20 pm - 12:30 pmDiscussion
12:30 pmLunch
1:30 pm - 4:00 pmFree Time
3:00 pm - 4:00 pmPower Hour
The GRC Power Hour is an optional informal gathering open to all meeting participants. It is designed to help address the challenges women face in science and support the professional growth of women in our communities by providing an open forum for discussion and mentoring.
Organizer: Karen Fleming (Johns Hopkins University, USA)
4:00 pm - 6:00 pmPoster Session
6:00 pmDinner
7:30 pm - 9:30 pmMembrane Protein Structure Prediction and Design
Discussion Leader: Hang "Hubert" Yin (University of Colorado Boulder, USA)
7:30 pm - 7:55 pmJens Meiler (Vanderbilt University, USA)
"Simulating Membrane Protein Folding from Limited Experimental Data"
7:55 pm - 8:05 pmDiscussion
8:05 pm - 8:30 pmSarel Fleishman (Weizmann Institute of Science, Israel)
"An Energy Function for Design and Structure Prediction in Membrane Proteins"
8:30 pm - 8:40 pmDiscussion
8:40 pm - 8:50 pmPatrick Barth (Baylor College of Medicine, USA)
"Enhancing Membrane Protein Structure Prediction and Design with Explicit Solvent-Protein Interactions"
8:50 pm - 8:55 pmDiscussion
8:55 pm - 9:20 pmPeter Woylnes (Rice University, USA)
"Predictive Energy Landscapes for Folding Membrane Proteins"
9:20 pm - 9:30 pmDiscussion
Tuesday
7:30 am - 8:30 amBreakfast
9:00 am - 12:30 pmMembrane Protein Folding in Disease
Discussion Leader: Radhakrishnan Mahalakshmi (Indian Institute of Science Education and Research, Bhopal, India)
9:00 am - 9:25 amJonathan Schlebach (Indiana University, USA)
"Elucidation of Bottlenecks in the Quality Control and Cellular Trafficking of PMP22"
9:25 am - 9:35 amDiscussion
9:35 am - 9:45 amOliver Zerbe (University of Zurich, Switzerland)
"NMR Methods to Study the Structure and Folding of GPCRs"
9:45 am - 9:50 amDiscussion
9:50 am - 10:15 amHang "Hubert" Yin (University of Colorado Boulder, USA)
"Rational Design of Membrane Curvature Sensors"
10:15 am - 10:25 amDiscussion
10:25 am - 10:35 amLaurent Catoire (CNRS / Paris Diderot University, France)
"Refolding of E. coli Expressed GPCR into Nanodisks Using Amphipols"
10:35 am - 10:40 amDiscussion
10:40 am - 11:05 amCoffee Break
11:05 am - 11:30 amGergely Lukacs (McGill University, Canada)
"Structural and Proteostatic Determinants of CFTR Functional Expression at the Cell Surface"
11:30 am - 11:40 amDiscussion
11:40 am - 11:50 amMichael Schlierf (TU Dresden, Germany)
"smFRET Reveals Structural Basis for Conformational Misfolding of a Cystic Fibrosis Mutation in CFTR"
11:50 am - 11:55 amDiscussion
11:55 am - 12:20 pmSabine Hadida (Vertex Pharmaceuticals, USA)
"Correcting CFTR Protein Processing and Trafficking to Treat the Underlying Cause of Cystic Fibrosis"
12:20 pm - 12:30 pmDiscussion
12:30 pmLunch
1:30 pm - 4:00 pmFree Time
4:00 pm - 6:00 pmPoster Session
6:00 pmDinner
7:30 pm - 9:30 pmMembrane Protein Stabilization
Discussion Leader: Karen Fleming (Johns Hopkins University, USA)
7:30 pm - 7:55 pmPil Seok Chae (Hanyang University, South Korea)
"Novel Detergent Tools for GPCR Structural Study"
7:55 pm - 8:05 pmDiscussion
8:05 pm - 8:30 pmLinda Columbus (University of Virginia, USA)
"Protein-Surfactant Interactions Do Determine Fold"
8:30 pm - 8:40 pmDiscussion
8:40 pm - 8:50 pmAmanda Duran (Vanderbilt University, USA)
"An Improved Rosetta Energy Function for Predicting Mutation-Induced Stability Changes in Membrane Proteins"
8:50 pm - 8:55 pmDiscussion
8:55 pm - 9:20 pmAndreas Pluckthun (University of Zurich, Switzerland)
"Stabilizing GPCRs"
9:20 pm - 9:30 pmDiscussion
Wednesday
7:30 am - 8:30 amBreakfast
9:00 am - 12:30 pmFolding of Beta Barrels and Others
Discussion Leader: Linda Columbus (University of Virginia, USA)
9:00 am - 9:25 amKaren Fleming (Johns Hopkins University, USA)
"Driving Forces for Sorting and Folding of Outer Membrane Proteins"
9:25 am - 9:35 amDiscussion
9:35 am - 9:45 amAntoniya Aleksandrova (National Institute of Neurological Disorders and Stroke, NIH, USA)
"Systematic Analysis of Symmetry in Membrane Proteins"
9:45 am - 9:50 amDiscussion
9:50 am - 10:15 amZengyi Chang (Peking University, China)
"The Biogenesis and Quality Control of Beta Barrel Outer Membrane Proteins as Explored in Living Cells"
10:15 am - 10:25 amDiscussion
10:25 am - 10:35 amWilliam Wimley (Tulane University School of Medicine, USA)
"Synthetic Molecular Evolution of Membrane Active Peptides"
10:35 am - 10:40 amDiscussion
10:40 am - 11:05 amCoffee Break
11:05 am - 11:30 amRadhakrishnan Mahalakshmi (Indian Institute of Science Education and Research, Bhopal, India)
"Interface Energetics and Beta Barrel Folding"
11:30 am - 11:40 amDiscussion
11:40 am - 11:50 amMatthias Buck (Case Western Reserve University, USA)
"Dynamic Protein-Membrane Complexes: NMR and Simulation Studies on Transmembrane Receptors and Ras GTPases"
11:50 am - 11:55 amDiscussion
11:55 am - 12:20 pmSheena Radford (University of Leeds, United Kingdom)
"Mechanistic Insights into Outer Membrane Protein Folding"
12:20 pm - 12:30 pmDiscussion
12:30 pmLunch
1:30 pm - 4:00 pmFree Time
4:00 pm - 6:00 pmPoster Session
6:00 pmDinner
7:00 pm - 7:30 pmBusiness Meeting
Nominations for the Next Vice Chair; Fill in Conference Evaluation Forms; Discuss Future Site and Scheduling Preferences; Election of the Next Vice Chair
7:30 pm - 9:30 pmMembrane Protein Folding in Amyloid Disease
Discussion Leader: Charles Sanders (Vanderbilt University, USA)
7:30 pm - 7:55 pmCeline Galvagnion (German Center for Neurodegenerative Diseases (DZNE), Germany)
"Amyloid Proteins - Membrane Interactions: The Influence of the Lipid Composition on the Kinetics of Aggregation"
7:55 pm - 8:05 pmDiscussion
8:05 pm - 8:30 pmKatsumi Matsuzaki (Graduate School of Pharmaceutical Sciences, Kyoto University, Japan)
"Mechanisms of Abnormal Aggregation and Toxicity of Alzheimer's Amyloid Beta-Protein on Neuronal Membranes"
8:30 pm - 8:40 pmDiscussion
8:40 pm - 8:50 pmAntje Pokorny (University of North Carolina at Wilmington, USA)
"Isoleucine-Rich Amphipathic Peptides Bind Significantly Better to Lipid Bilayers than Leucine-Rich Peptides"
8:50 pm - 8:55 pmDiscussion
8:55 pm - 9:20 pmJennifer Lee (National Institutes of Health, USA)
"Membrane Interactions and Amyloid Formation of α-Synuclein"
9:20 pm - 9:30 pmDiscussion
Thursday
7:30 am - 8:30 amBreakfast
9:00 am - 12:30 pmAssembly of Membrane Protein Complexes
Discussion Leader: Paula Booth (King's College London, United Kingdom)
9:00 am - 9:25 amCharles Brooks (University of Michigan, USA)
"The Role of pH in Modulating Membrane Protein Structure and Function"
9:25 am - 9:35 amDiscussion
9:35 am - 9:45 amRaphael Trenker (The Walter and Eliza Hall Institute of Medical Research, Australia)
"Screening and Crystallographic Analysis of Intramembrane Helix Interactions Using Lipidic Cubic Phase (LCP) Techniques"
9:45 am - 9:50 amDiscussion
9:50 am - 10:15 amAdam Smith (University of Akron, USA)
"Resolving the Dimerization Interface of Visual Opsins with PIE-FCCS"
10:15 am - 10:25 amDiscussion
10:25 am - 10:45 amCoffee Break
10:45 am - 11:10 amCharles Deber (University of Toronto, Canada)
"Peptide-Based Approaches to Inhibiting Protein-Protein Interactions in Membranes"
11:10 am - 11:20 amDiscussion
11:20 am - 11:45 amArt Laganowski (Texas A&M University, USA)
"Probing Membrane Protein-Lipid Interactions by Native Ion Mobility Mass Spectrometry"
11:45 am - 11:55 amDiscussion
11:55 am - 12:20 pmWilliam Degrado (University of California, San Francisco, USA)
"Understanding Transmembrane Signal Transduction in Bacterial Histidine Kinases"
12:20 pm - 12:30 pmDiscussion
12:30 pmLunch
1:30 pm - 4:00 pmFree Time
4:00 pm - 6:00 pmPoster Session
6:00 pmDinner
7:30 pm - 9:30 pmMembrane Protein Design for Disease Therapy
Discussion Leader: Kalina Hristova (Johns Hopkins University, USA)
7:30 pm - 7:55 pmDaniel Dimaio (Yale University, USA)
"Building Transmembrane Proteins with Minimal Chemical Complexity"
7:55 pm - 8:05 pmDiscussion
8:05 pm - 8:30 pmKalina Hristova (Johns Hopkins University, USA)
"Heterodimerization of Receptor Tyrosine Kinases in Health and Disease"
8:30 pm - 8:40 pmDiscussion
8:40 pm - 8:50 pmJonathan Sachs (University of Minnesota, USA)
"Thermodynamics of Transmembrane Domains Can Be Exploited to Manipulate TNF Receptor Function"
8:50 pm - 8:55 pmDiscussion
8:55 pm - 9:20 pmDonald Engelman (Yale University, USA)
"pHLIP®: Uses in Measuring Cell Surface pH, Imaging Tumors, and Delivering Therapeutics"
9:20 pm - 9:30 pmDiscussion
Friday
7:30 am - 8:30 amBreakfast
9:00 amDeparture
© 2017 Gordon Research Conferences Search | Contact | Terms of Use | Privacy Policy | Follow us: Facebook Twitter