Sunday
2:00 pm - 9:00 pm
Arrival and Check-in
6:00 pm - 7:00 pm
Dinner
7:30 pm - 7:40 pm
Introductory Comments by GRC Staff / Welcome and Introduction from the Chairs
7:40 pm - 9:30 pm
Protease-Targeting Therapeutics
Discussion Leader: Guy Salvesen (Sanford Burnham Prebys Medical Discovery Institute, United States)
7:40 pm - 7:45 pm
Introduction by Discussion Leader
7:45 pm - 8:05 pm
Irit Sagi (The Weizmann Institute of Science, Israel)
"Targeting Pathophysiological Proteolysis Utilizing Smart Bacterial Delivery System"
8:05 pm - 8:15 pm
Discussion
8:15 pm - 8:30 pm
Mark Gorrell (Centenary Institute, The University of Sydney, Australia)
"Dipeptidyl Peptidase (DPP) 4 Family Inhibition Retards Tumor Growth in a Syngeneic Susbcutaneous Liver Tumor Model"
8:30 pm - 8:35 pm
Discussion
8:35 pm - 8:50 pm
Late-Breaking Topic
8:50 pm - 8:55 pm
Discussion
8:55 pm - 9:15 pm
Celia Schiffer (University of Massachusetts Chan Medical School, United States)
"Pre-Emptively Avoiding Drug Resistance: Lessons from Viral Proteases: From HIV to SARS-CoV-2"
9:15 pm - 9:25 pm
Discussion
9:25 pm - 9:30 pm
General Discussion
Monday
7:30 am - 8:30 am
Breakfast
9:00 am - 12:30 pm
Proteases in Pathogens
Discussion Leader: Matthew Bogyo (Stanford University School of Medicine, United States)
9:00 am - 9:05 am
Introduction by Discussion Leader
9:05 am - 9:25 am
Christopher Overall (University of British Columbia, Canada)
"The Great Escape of SARS-CoV-2 by 3CLpro"
9:25 am - 9:35 am
Discussion
9:35 am - 9:55 am
Dusan Turk (Jozef Stefan Institute, Slovenia)
"Quest for Inhibition of Cell Entry of SARS-CoV-2 and Other Viruses"
9:55 am - 10:05 am
Discussion
10:05 am - 10:15 am
Short Talk Selected from Poster Abstracts
10:15 am - 10:20 am
Discussion
10:20 am - 10:45 am
Coffee Break
10:45 am - 10:55 am
Short Talk Selected from Poster Abstracts
10:55 am - 11:00 am
Discussion
11:00 am - 11:20 am
Joanne Lemieux (University of Alberta, Canada)
"Examining the Role of Rhomboid Protease in Bacterial Pathogenicity"
11:20 am - 11:30 am
Discussion
11:30 am - 11:40 am
Brianna Hurysz (UCSD, United States)
"Expressing and Characterizing the Trichomonas Vaginalis Proteasome for Improved Drug Development"
11:40 am - 11:45 am
Discussion
11:45 am - 12:05 pm
Jack Bravo (University of Texas at Austin, United States)
"A CRISPR-Cas Based Proteolytic Signalling Cascade for Bacterial Immunity"
12:05 pm - 12:15 pm
Discussion
12:15 pm - 12:30 pm
Poster Previews
12:30 pm - 1:30 pm
Lunch
1:30 pm - 4:30 pm
Free Time
3:00 pm - 4:00 pm
The GRC Power Hour™
The GRC Power Hour™ is designed to address diversity and inclusion in the scientific workplace by providing a safe environment for informal and meaningful conversations amongst colleagues of all career stages. The program supports the professional growth of all members of our communities, including ethnicity, race and/or gender identity by providing an open forum for discussion and mentoring.
Organizer: Catherine Moali (CNRS, University of Lyon, France)
4:30 pm - 6:00 pm
Poster Session
6:00 pm - 8:00 pm
New Means of Metabolic and Small Molecule Protease Regulation
Discussion Leader: Daniel Sojka (Institute of Parasitology BC CAS, Ceske Budejovice , Czech Republic)
6:00 pm - 6:05 pm
Introduction by Discussion Leader
6:05 pm - 6:25 pm
Ami Navon (Bar-Ilan-University, Israel)
"An Internal Oxidative Switch Regulates Proteasome Reprogramming"
6:25 pm - 6:35 pm
Discussion
6:35 pm - 6:45 pm
Klara Grantz Saskova (Charles University, IOCB Prague, Czech Republic)
"Developing Treatment of Neurodegenerative Diseases Associated with Protein Aggregation"
6:45 pm - 6:50 pm
Discussion
6:50 pm - 7:00 pm
Late-Breaking Topic
7:00 pm - 7:05 pm
Discussion
7:05 pm - 7:25 pm
Dieter Bromme (University of British Columbia, Canada)
"Are we Missing a Major Aspect in Protease Research?"
7:25 pm - 7:30 pm
Discussion
7:30 pm - 7:50 pm
Tania Baker (Massachusetts Institute of Technology, United States)
"By Encoding Two Distinct ClpP Peptidases Pseudomonas Aeruginosa Can Re-Calibrate Degradation of Multiple Substrates to Enhance Virulence"
7:50 pm - 8:00 pm
Discussion
8:00 pm - 9:00 pm
Dinner
Tuesday
7:30 am - 8:30 am
Breakfast
8:30 am - 9:00 am
Group Photo
9:00 am - 12:30 pm
Contributions of Proteases to Diverse Diseases
Discussion Leader: Henry Maun (Genentech, United States)
9:00 am - 9:05 am
Introduction by Discussion Leader
9:05 am - 9:25 am
Niki Chondrogianni (National Hellenic Research Foundation, Greece)
"Proteasome Activation: Unravelling the Mechanisms and the Outcomes in Ageing and Age-Related Diseases"
9:25 am - 9:35 am
Discussion
9:35 am - 9:45 am
Late-Breaking Topic
9:45 am - 9:50 am
Discussion
9:50 am - 10:10 am
James Whisstock (Monash University, Australia)
"New Antibody Derived Inhibitors of the Plasminogen System"
10:10 am - 10:15 am
Discussion
10:15 am - 10:25 am
Wanjian (Jocelyn) Tang (Genentech, United States)
"Inhibiting Human Neutrophil Serine Protease 4 with mRNA Display-Derived Macrocyclic Peptides"
10:25 am - 10:30 am
Discussion
10:30 am - 11:00 am
Coffee Break
11:00 am - 11:20 am
Vinit Mahajan (Stanford University, United States)
"Targeting Calpain-5 Hyperactivating Mutations with Peptidomimetic Inhibitors for the Treatment of Human Retinal Disease"
11:20 am - 11:25 am
Discussion
11:25 am - 11:40 am
Sabrina Sofia Burgener (Institute for Molecular Bioscience, The University of Queensland, Brisbane, Australia)
"Caspase-1 Self-Deactivation Terminates Protease Activity to Promote Tissue Resolution in Chronic Liver Disease"
11:40 am - 11:45 am
Discussion
11:45 am - 12:05 pm
James Huntington (University of Cambridge, United Kingdom)
"Prothrombinase: What We Can Learn from Venomous Snakes"
12:05 pm - 12:15 pm
Discussion
12:15 pm - 12:30 pm
Poster Previews
12:30 pm - 1:30 pm
Lunch
1:30 pm - 4:30 pm
Free Time
4:30 pm - 6:00 pm
Poster Session
6:00 pm - 8:00 pm
Exosites and Dynamics in Protease Biology
Discussion Leader: Ruth Geiss-Friedlander (Albert-Ludwigs-University Freiburg, Germany)
6:00 pm - 6:20 pm
Evette Radisky (Mayo Clinic, United States)
"Allosteric Inhibition of Mesotrypsin, an Oncogenic Human Trypsin Isoform"
6:20 pm - 6:25 pm
Discussion
6:25 pm - 6:45 pm
Dan Herschlag (Stanford University, United States)
"A Comprehensive Model for Serine Protease Catalysis Derived from Ensemble-Function Analysis"
6:45 pm - 6:55 pm
Discussion
6:55 pm - 7:15 pm
Carla Mattos (Northeastern University, United States)
"MSCS Analysis of the Binding Surfaces of Trypsin, Chymotrypsin and Elastase"
7:15 pm - 7:25 pm
Discussion
7:25 pm - 7:35 pm
Short Talk Selected from Poster Abstracts
7:35 pm - 7:40 pm
Discussion
7:40 pm - 7:55 pm
Henry Maun (Genentech, United States)
"Mechanistic and Structural Studies of a Novel Inhibitory Anti-Tryptase Antibody Delivering an Allosteric “Gut Punch” to the Target"
7:55 pm - 8:00 pm
Discussion
8:00 pm - 9:00 pm
Dinner
Wednesday
7:30 am - 8:30 am
Breakfast
9:00 am - 12:30 pm
Emerging Technologies for Probing Proteases
Discussion Leader: Anthony O'Donoghue (University of California, San Diego, United States)
9:00 am - 9:05 am
Introduction by Discussion Leader
9:05 am - 9:25 am
Min Chen (University of Massachusetts Amherst, United States)
"Characterizing Conformational Dynamic of Flaviviral Proteases: Single-Molecule Observation Using Nanopore Tweezers"
9:25 am - 9:35 am
Discussion
9:35 am - 9:55 am
Late-Breaking Topic
9:55 am - 10:00 am
Discussion
10:00 am - 10:15 am
Euna Yoo (National Cancer Institute, NIH, United States)
"Development of ISG15-Based Probes for Selective USP18 Activity Profiling"
10:15 am - 10:20 am
Discussion
10:20 am - 10:30 am
Short Talk Selected from Poster Abstracts
10:30 am - 10:35 am
Discussion
10:35 am - 11:05 am
Coffee Break
11:05 am - 11:25 am
Manu Platt (National Institutes of Health, United States)
"Building Dynamic Models of Proteolytic Networks Considering Protease-on-Protease Interactive Hydrolysis to Identify Inhibitory Strategies in Multi-Protease Systems"
11:25 am - 11:30 am
Discussion
11:30 am - 11:40 am
Short Talk Selected from Poster Abstracts
11:40 am - 11:45 am
Discussion
11:45 am - 12:05 pm
Ronit Satchi-Fainaro (Tel Aviv University, Israel)
"D-Bioprinted Cancer Models for Target Discovery, Drug Development, and Personalized Therapy"
12:05 pm - 12:10 pm
Discussion
12:10 pm - 12:30 pm
Poster Previews
12:30 pm - 1:30 pm
Lunch
1:30 pm - 4:30 pm
Free Time
4:30 pm - 6:00 pm
Poster Session
6:00 pm - 8:00 pm
Engineering and Evolution of New Protease Function
Discussion Leader: Galia Blum (Hebrew University of Jerusalem, Israel)
6:00 pm - 6:05 pm
Introduction by Discussion Leader
6:05 pm - 6:25 pm
Gregory Weiss (University of California, Irvine, United States)
"Directed Evolution of the Super-Specific Botox Protease"
6:25 pm - 6:35 pm
Discussion
6:35 pm - 6:50 pm
Carl Denard (University of Florida, United States)
"High-Throughput Functional Selection for Protease Modulatory Protein Binders"
6:50 pm - 6:55 pm
Discussion
6:55 pm - 7:10 pm
Maryam Raeeszadeh-Sarmazdeh (University of Nevada, Reno, United States)
"Developing Protein Therapeutics Based on Metalloproteinase Inhibitors"
7:10 pm - 7:15 pm
Discussion
7:15 pm - 7:25 pm
Sarah Alamdari (Microsoft Research, United States)
"In-Silico Design of Protease-Cleavable Peptide Substrates"
7:25 pm - 7:30 pm
Discussion
7:30 pm - 7:50 pm
JP Maianti (Albert Einstein College of Medicine and Montefiore-Einstein Comprehensive Cancer Center, United States)
"Targeting the Exo-Site of the Metalloprotease Insulin-Degrading Enzyme Enables Potent, Specific, and Substrate-Selective Inhibition"
7:50 pm - 8:00 pm
Discussion
8:00 pm - 9:00 pm
Dinner
Thursday
7:30 am - 8:30 am
Breakfast
8:30 am - 9:00 am
Business Meeting
Nominations for the Next Vice Chair(s); Complete the GRC Evaluation Forms; Discuss Future Dates and Venue; Election of the Next Vice Chair(s)
9:00 am - 12:30 pm
Session in Memory of Margarete Heck: Crosstalk of Proteases in Cancer and Inflammatory Diseases
Discussion Leader: Klaudia Brix (Constructor University , Germany)
9:00 am - 9:10 am
Introduction by Discussion Leader
9:10 am - 9:30 am
Michelle Arkin (University of California, San Francisco, United States)
"Roles of Caspase-6 in Alzheimer’s Disease"
9:30 am - 9:40 am
Discussion
9:40 am - 9:55 am
Ozlem Dogan Ekici (The Ohio State University, United States)
"Aza-Peptide Michael Acceptors as Inhibitors of the Human 20S Proteasome"
9:55 am - 10:00 am
Discussion
10:00 am - 10:20 am
Tsan Xiao (Case Western Reserve University, United States)
"Dual-Site Recognition of Substrates by Caspases"
10:20 am - 10:30 am
Discussion
10:30 am - 11:00 am
Coffee Break
11:00 am - 11:20 am
Cornelius Taabazuing (University of Pennsylvania, United States)
"New Insights into Inflammatory Caspase Substrate Specificities"
11:20 am - 11:25 am
Discussion
11:25 am - 11:35 am
Short Talk Selected from Poster Abstracts
11:35 am - 11:40 am
Discussion
11:40 am - 11:55 am
Reid Alderson (Helmholtz Munich, Germany)
"Activation of Caspase-9 on the Apoptosome: An NMR View of the Flexibly Tethered Protease Domain"
11:55 am - 12:00 pm
Discussion
12:00 pm - 12:20 pm
Keqiang Ye (Shenzhen Institute of Advanced Technology, China)
"Blockade of Asparagine Endopeptidase for Treating Age-Related Diseases"
12:20 pm - 12:30 pm
Discussion
12:30 pm - 1:30 pm
Lunch
1:30 pm - 4:30 pm
Free Time
4:30 pm - 6:00 pm
Poster Session
6:00 pm - 8:00 pm
Protease Profiling and Substrate Selection with Lessons from Auf Dem Keller
Discussion Leader: Antoine Dufour (University of Calgary, Canada)
6:00 pm - 6:10 pm
Introduction by Discussion Leader
6:10 pm - 6:30 pm
Olivier Julien (University of Alberta, Canada)
"Characterizing Proteolysis During Apoptosis and Viral Infections"
6:30 pm - 6:40 pm
Discussion
6:40 pm - 6:55 pm
Kristyna Blazkova (Stanford University, United States)
"Identification of Proteases Produced by Commensal Bacteria of the Human Microbiota that Cleave and Regulate Protease-Activated Receptor 2 to Control Pain Signaling in the Gut"
6:55 pm - 7:00 pm
Discussion
7:00 pm - 7:20 pm
Late-Breaking Topic
7:20 pm - 7:25 pm
Discussion
7:25 pm - 7:45 pm
Stefan Lichtenthaler (German Center for Neurodegenerative Diseases (DZNE), Germany)
"Ectodomain Shedding in Alzheimer's Disease: From Substrate Identification to Clinical Trials"
7:45 pm - 7:55 pm
Discussion
7:55 pm - 8:00 pm
General Discussion
8:00 pm - 9:00 pm
Dinner
Friday
7:30 am - 8:30 am
Breakfast
9:00 am
Departure