Sunday
2:00 pm - 9:00 pm
Arrival and Check-in
6:00 pm - 7:00 pm
Dinner
7:30 pm - 7:40 pm
Introductory Comments by GRC Staff / Welcome and Introduction from the Chairs
7:40 pm - 9:30 pm
Vision for the Plant Cell Atlas
Discussion Leader: Samuel Leiboff (Oregon State University, United States)
7:40 pm - 8:10 pm
Ru Gunawardane (Allen Institute for Cell Science, United States)
"Creating a Spatiotemporal Map of Cell States Using hiPSCs Towards Understanding the Principles that Govern Cellular Organization, Behavior, and Identity"
8:10 pm - 8:20 pm
Discussion
8:20 pm - 8:50 pm
Philip Benfey (Duke University / Howard Hughes Medical Institute, United States)
"Root Dynamics at Single Cell Resolution"
8:50 pm - 9:00 pm
Discussion
9:00 pm - 9:10 pm
Song Li (Virginia Tech, United States)
"Cross-Species Comparison of Single-Cell Genomic Data in Plants"
9:10 pm - 9:15 pm
Discussion
9:15 pm - 9:25 pm
Natanella Illouz-Eliaz (The Salk Institute for Biological Studies, United States)
"Understanding Drought Stress and Recovery at Single-Cell Resolution"
9:25 pm - 9:30 pm
Discussion
Monday
7:30 am - 8:30 am
Breakfast
8:30 am - 9:00 am
Group Photo
9:00 am - 12:30 pm
Characterizing Plant Developmental Programs at the Single-Cell Level
Discussion Leaders: Marc Libault (University of Nebraska Lincoln, United States) and Benjamin Cole (DOE-Joint Genome Institute, United States)
9:00 am - 9:10 am
Introduction by Discussion Leader
9:10 am - 9:40 am
Joseph Ecker (Salk Institute for Biological Studies and Howard Hughes Medical Institute, United States)
"Deconstructing Plant Processes: Cell by Cell"
9:40 am - 9:50 am
Discussion
9:50 am - 10:20 am
Jennifer Nemhauser (U. Washington, United States)
"What Do I Do Now? Validating Candidates Using Cell-type Specific Modulation of Function"
10:20 am - 10:30 am
Discussion
10:30 am - 10:55 am
Coffee Break
10:55 am - 11:25 am
Cole Trapnell (University of Washington, United States)
"Whole-Embryo Developmental Genetics at Single-Cell Resolution"
11:25 am - 11:35 am
Discussion
11:35 am - 11:45 am
Alex Canto Pastor (UC Davis, United States)
"Single Cell Transcriptomics as a Tool to Uncover the Function and Regulation Apoplastic Barriers in the Tomato Root"
11:45 am - 11:50 am
Discussion
11:50 am - 12:00 pm
Caroline Martin (MIT, United States)
"Mapping Signatures of Selection to Cell Types at the Maternal-Offspring Interface of Arabidopsis Thaliana "
12:00 pm - 12:05 pm
Discussion
12:05 pm - 12:30 pm
Poster Previews
12:30 pm - 1:30 pm
Lunch
1:30 pm - 4:00 pm
Free Time
3:00 pm - 4:00 pm
The GRC Power Hourâ„¢
The GRC Power Hourâ„¢ is designed to address diversity and inclusion in the scientific workplace by providing a safe environment for informal and meaningful conversations amongst colleagues of all career stages. The program supports the professional growth of all members of our communities, including ethnicity, race and/or gender identity by providing an open forum for discussion and mentoring.
Organizers: Yoselin Benitez Alfonso (University of Leeds, United Kingdom), Noah Fahlgren (Donald Danforth Plant Science Center, United States) and Rachel Shahan (Duke University, United States)
4:00 pm - 6:00 pm
Poster Session
6:00 pm - 7:00 pm
Dinner
7:30 pm - 9:30 pm
Multiscale and Quantitative Imaging for Single Cells
Discussion Leader: David Ehrhardt (Carnegie Institution for Science / Stanford University, United States)
7:30 pm - 7:40 pm
Introduction by Discussion Leader
7:40 pm - 8:10 pm
Sylvia Rodrigues da Silveira (University of Montreal, Canada)
"Mechanical Interactions and Differential Growth Dynamics Between Tissue Layers in Stamen Morphogenesis"
8:10 pm - 8:20 pm
Discussion
8:20 pm - 8:50 pm
Florent Waltz (University of Basel, Switzerland)
"High Resolution Investigation of Organelle Molecular Architecture using Cryo-Electron Tomography"
8:50 pm - 9:00 pm
Discussion
9:00 pm - 9:10 pm
Kevin Cox (Donald Danforth Plant Science Center, United States)
"Expansion Microscopy to Visualize Subcellular Components in Plants"
9:10 pm - 9:15 pm
Discussion
9:15 pm - 9:25 pm
Colleen Drapek (University of Cambridge, United Kingdom)
"Cellular Gibberellin Dynamics During Beneficial Root-Microbe Interactions"
9:25 pm - 9:30 pm
Discussion
Tuesday
7:30 am - 8:30 am
Breakfast
9:00 am - 12:30 pm
Spatially Defining the Proteome Landscape in Plant Cells
Discussion Leader: R. Glen Uhrig (University of Alberta, Canada)
9:00 am - 9:10 am
Introduction by Discussion Leader
9:10 am - 9:40 am
Sixue Chen (University of Mississippi, United States)
"Single Cell-Type Proteomics and Metabolomics of Stomatal Disease Triangle"
9:40 am - 9:50 am
Discussion
9:50 am - 10:20 am
Amir Ahkami (Pacific Northwest National Laboratory, United States)
"Single-Cell-Type Proteomics Identifies Novel Molecular Determinants of Plant Responses to Abiotic Stresses"
10:20 am - 10:30 am
Discussion
10:30 am - 10:55 am
Coffee Break
10:55 am - 11:25 am
Justin Walley (Iowa State University, United States)
"Plant Single Cell Proteomics: Challenges and Opportunities"
11:25 am - 11:35 am
Discussion
11:35 am - 11:45 am
Matias Zurbriggen (University of Düsseldorf, Germany)
"Plant Synthetic and Reconstruction Biology Approaches for the Study and Control of Cellular Processes in Plants"
11:45 am - 11:50 am
Discussion
11:50 am - 12:00 pm
Maite Colinas (Max Planck Institute for Chemical Ecology, Germany)
"Proteomics of Cell Type Specific Stress Responses in the Arabidopsis Root Using Protein Proximity Labelling"
12:00 pm - 12:05 pm
Discussion
12:05 pm - 12:30 pm
Poster Previews
12:30 pm - 1:30 pm
Lunch
1:30 pm - 4:00 pm
Free Time
4:00 pm - 6:00 pm
Poster Session
6:00 pm - 7:00 pm
Dinner
7:30 pm - 9:30 pm
Spatially Resolved Single Cell Metabolomics
Discussion Leader: Christopher Anderton (Pacific Northwest National Laboratory, United States)
7:30 pm - 7:40 pm
Introduction by Discussion Leader
7:40 pm - 8:10 pm
Asaph Aharoni (Weizmann Institute of Science, Israel)
"Metabolic Profiling of Organelles at the Cell Type Level"
8:10 pm - 8:20 pm
Discussion
8:20 pm - 8:50 pm
Aleksandra Skirycz (Boyce Thompsosn Institute, United States)
"Functional Characterization of Metabolites Via Identification of Protein-Metabolite Interactions"
8:50 pm - 9:00 pm
Discussion
9:00 pm - 9:10 pm
Chenxin Li (University of Georgia, United States)
"Cell Type Aware Regulatory Landscape of Specialized Metabolism"
9:10 pm - 9:15 pm
Discussion
9:15 pm - 9:25 pm
Tao Zhang (UC San Diego, United States)
"Chemical Imaging Reveals Diverse Functions of Tricarboxylic Acid Metabolites in Root Growth and Development"
9:25 pm - 9:30 pm
Discussion
Wednesday
7:30 am - 8:30 am
Breakfast
9:00 am - 12:30 pm
Automating Phenotyping at Cellular, Organ, and Whole Organism Levels
Discussion Leader: Margaret Frank (Cornell University, United States)
9:00 am - 9:10 am
Introduction by Discussion Leader
9:10 am - 9:40 am
Malia Gehan (Donald Danforth Plant Science Center, United States)
"What Phenotypes Matter? Open Questions in Plant Phenotyping with PlantCV"
9:40 am - 9:50 am
Discussion
9:50 am - 10:20 am
Siobhan Braybrook (UCLA, United States)
"When & Where to Look? Quantifying Growth on the Right Scale at the Right Time and in the Right Place"
10:20 am - 10:30 am
Discussion
10:30 am - 10:55 am
Coffee Break
10:55 am - 11:25 am
Anne-Lise Routier-Kierzkowska (University of Montreal, IRBV, Canada)
"How Fast Do Plant Cells Really Grow? A Meta-Analysis Across Organ and Cell Identities"
11:25 am - 11:35 am
Discussion
11:35 am - 11:45 am
Noe Perron (University of Florida, United States)
"Single-Cell Study of C3 to CAM Transition in the Ice Plant Points to New Strategies for Increasing Drought Tolerance"
11:45 am - 11:50 am
Discussion
11:50 am - 12:00 pm
Byron Rusnak (Cornell University, United States)
"A Spatially Resolved Single Cell View of Sepal Initiation"
12:00 pm - 12:05 pm
Discussion
12:05 pm - 12:30 pm
Poster Previews
12:30 pm - 1:30 pm
Lunch
1:30 pm - 4:00 pm
Free Time
4:00 pm - 6:00 pm
Poster Session
6:00 pm - 7:00 pm
Dinner
7:30 pm - 9:30 pm
Single Cell Comparative Plant Cell Biology and Evolution
Discussion Leader: Michelle Facette (University of Massachusetts, Amherst, United States)
7:30 pm - 7:40 pm
Introduction by Discussion Leader
7:40 pm - 8:10 pm
Joyce Onyenedum (Cornell University, United States)
"Unraveling the Developmental Processes Underlying the Twining Motion of Vines"
8:10 pm - 8:20 pm
Discussion
8:20 pm - 8:50 pm
Masayuki Onishi (Duke University, United States)
"Cytokinesis in Chlamydomonas : Animal-like Furrowing Using Plant-like Components"
8:50 pm - 9:00 pm
Discussion
9:00 pm - 9:10 pm
Irene Liao (University of California, Los Angeles, United States)
"Candidate Genes Underlying Nectary Development in Monocots"
9:10 pm - 9:15 pm
Discussion
9:15 pm - 9:25 pm
John Pablo Mendieta (University of Georgia, United States)
"Deciphering the Evolutionary History of Cell-Type-Specific Accessible Chromatin Regions by Comparative Genomic Approaches"
9:25 pm - 9:30 pm
Discussion
Thursday
7:30 am - 8:30 am
Breakfast
8:30 am - 9:00 am
Business Meeting
Nominations for the Next Vice Chair(s); Complete the GRC Evaluation Forms; Discuss Future Dates and Venue; Election of the Next Vice Chair(s)
9:00 am - 12:30 pm
Systems and Synthetic Biology Approaches for Enabling Single Cell Biology
Discussion Leaders: Jenn Brophy (Stanford University, United States)
9:00 am - 9:10 am
Introduction by Discussion Leader
9:10 am - 9:40 am
Gözde Demirer (Caltech, United States)
"High-Throughput Resources to Study Plant Transcriptional Regulatory Elements"
9:40 am - 9:50 am
Discussion
9:50 am - 10:20 am
Aditya Kunjapur (University of Delaware, United States)
"Engineering Biological Containment and Effector Biosynthesis in Bacillus subtilis for Crop Protection"
10:20 am - 10:30 am
Discussion
10:30 am - 11:00 am
Coffee Break
11:00 am - 11:30 am
Sean Cutler (University of California - Riverside, United States)
"Plant Hormone Sensors as Programmable Chemical Induced Dimerization Modules"
11:30 am - 11:40 am
Discussion
11:40 am - 11:50 am
Dick Roelofs (KeyGene, The Netherlands)
"Establishing Spatial Transcriptomics for Crop Improvement"
11:50 am - 11:55 am
Discussion
11:55 am - 12:05 pm
Karen Serrano (University of California - Berkeley, United States)
"Spatially-Resolved, Single Cell Transcriptome Mapping of the Arbuscular Mycorrhizal Symbiosis"
12:05 pm - 12:10 pm
Discussion
12:10 pm - 12:30 pm
General Discussion
12:30 pm - 1:30 pm
Lunch
1:30 pm - 4:00 pm
Free Time
4:00 pm - 6:00 pm
Poster Session
6:00 pm - 7:00 pm
Dinner
7:30 pm - 9:30 pm
Modeling and Data Integration for Knowledge Synthesis from Single Cell Datasets
Discussion Leaders: Noah Fahlgren (Donald Danforth Plant Science Center, United States)
7:30 pm - 7:40 pm
Introduction by Discussion Leader
7:40 pm - 8:10 pm
Robert Schmitz (University of Georgia, United States)
"Deciphering cis -Regulatory Elements at Single-Cell Resolution in Plants"
8:10 pm - 8:20 pm
Discussion
8:20 pm - 8:50 pm
Shao-Shan Carol Huang (New York University, United States)
"Robust Discovery of Gene Regulatory Networks from Single-Cell Gene Expression Data Using Causal Inference with Composition of Transactions"
8:50 pm - 9:00 pm
Discussion
9:00 pm - 9:10 pm
Short Talk Selected from Poster Abstracts
9:10 pm - 9:15 pm
Discussion
9:15 pm - 9:25 pm
Michael Passalacqua (Cold Spring Harbor Laboratory, United States)
"Leveraging Coexpression to Improve Cross-Species Single Cell Integration in Plants"
9:25 pm - 9:30 pm
Discussion
Friday
7:30 am - 8:30 am
Breakfast
9:00 am
Departure