Sunday
2:00 pm - 9:00 pm
Arrival and Check-in
6:00 pm - 7:00 pm
Dinner
7:30 pm - 7:40 pm
Introductory Comments by GRC Site Staff / Welcome from the GRC Chair
7:40 pm - 9:30 pm
Keynote Session: Challenging Specimens
7:40 pm - 7:50 pm
Introduction by Discussion Leader
7:50 pm - 8:25 pm
"When Your Sample Just Won't Freeze (Literally and Metaphorically)"
8:25 pm - 8:40 pm
Discussion
8:40 pm - 9:15 pm
"Time Resolved Imaging of Ultrafast Synaptic Vesicle Endocytosis"
9:15 pm - 9:30 pm
Discussion
Monday
7:30 am - 8:30 am
Breakfast
9:00 am - 12:30 pm
Cryo-EM in the Context of Developing Therapeutics
9:00 am - 9:20 am
Introduction by Discussion Leader
9:20 am - 9:50 am
"Ligand Modeling in Near-Atomic Resolution Cryo-EM Maps and Its Application to Structure-Based Drug Discovery at Schrodinger"
9:50 am - 10:00 am
Discussion
10:00 am - 10:30 am
"Structural Vaccinology: A Three Dimensional View for Vaccine Development"
10:30 am - 10:40 am
Discussion
10:40 am - 11:10 am
Coffee Break
11:10 am - 11:40 am
"Cryo-EM in the Fight Against Malaria"
11:40 am - 11:50 am
Discussion
11:50 am - 12:20 pm
"Building Cryo-EM at Genentech to Enable Research and Pipeline Projects"
12:20 pm - 12:30 pm
Discussion
12:30 pm - 1:30 pm
Lunch
1:30 pm - 4:00 pm
Free Time
4:00 pm - 6:00 pm
Poster Session
6:00 pm - 7:00 pm
Dinner
7:30 pm - 9:30 pm
Advances in Specimen Preparation
7:30 pm - 7:45 pm
Introduction by Discussion Leader
7:45 pm - 8:10 pm
"A New Generation of Specimen Supports for Electron Cryomicroscopy"
8:10 pm - 8:20 pm
Discussion
8:20 pm - 8:45 pm
"Approaches to Screening and Isolation of Proteins, and Their Complexes, for Structural Studies"
8:45 pm - 8:55 pm
Discussion
8:55 pm - 9:20 pm
"Single Particle CryoEM of Integrin in an Extended Conformation"
9:20 pm - 9:30 pm
Discussion
Tuesday
7:30 am - 8:30 am
Breakfast
8:30 am - 9:00 am
Group Photo
9:00 am - 12:30 pm
9:00 am - 9:20 am
Introduction by Discussion Leader
9:20 am - 9:50 am
"Visualizing Mechanically-Regulated Conformational States"
9:50 am - 10:00 am
Discussion
10:00 am - 10:30 am
"Microscopy Studies of Malaria Parasites Breaking out of Red Blood Cells"
10:30 am - 10:40 am
Discussion
10:40 am - 11:10 am
Coffee Break
11:10 am - 11:40 am
"Helical Assemblies with Broken Symmetry"
11:40 am - 11:50 am
Discussion
11:50 am - 12:20 pm
"Assembly, Activation, and Allostery: Using CryoEM to Understand How Living Matter Works"
12:20 pm - 12:30 pm
Discussion
12:30 pm - 1:30 pm
Lunch
1:30 pm - 4:00 pm
Free Time
4:00 pm - 6:00 pm
Poster Session
6:00 pm - 7:00 pm
Dinner
7:30 pm - 9:30 pm
Selected Poster Presentations: Recent Breakthroughs in 3DEM
7:30 pm - 7:40 pm
"Multi-Pass (Electron) Microscopy for Low Damage Imaging Applications"
7:40 pm - 7:45 pm
Discussion
7:45 pm - 7:55 pm
"3D Structure of Tripartite Efflux System Sandwiched Between Two Nanodiscs"
7:55 pm - 8:00 pm
Discussion
8:00 pm - 8:10 pm
"Lipid Bilayer Structure in Native Cell Membrane Nanoparticles of Multidrug Exporter AcrB"
8:10 pm - 8:15 pm
Discussion
8:15 pm - 8:25 pm
"EM Manager Tools in Leginon/Appion"
8:25 pm - 8:30 pm
Discussion
8:30 pm - 8:40 pm
"Supporting Drug Discovery with Improved Software and Sample Preparation"
8:40 pm - 8:45 pm
Discussion
8:45 pm - 8:55 pm
"Putting the Pieces Together: Building a Composite EM Map of a Flexible Macromolecule from Multiple Focused Sub-Maps"
8:55 pm - 9:00 pm
Discussion
9:00 pm - 9:10 pm
"Single-Protein Detection in Cryo-EM Images of Crowded Cellular Environments"
9:10 pm - 9:15 pm
Discussion
9:15 pm - 9:25 pm
"Structural Studies of Lipid Flippases Using Cryo-Electron Microscopy"
9:25 pm - 9:30 pm
Discussion
Wednesday
7:30 am - 8:30 am
Breakfast
9:00 am - 12:30 pm
Frontiers in Electron Tomography
9:00 am - 9:20 am
Introduction by Discussion Leader
9:20 am - 9:50 am
"Structure-Function Studies Using Cellular Cryo-Electron Tomography"
9:50 am - 10:00 am
Discussion
10:00 am - 10:30 am
"Exploring the Molecular Landscape of Chlamydomonas with In Situ Cryo-ET"
10:30 am - 10:40 am
Discussion
10:40 am - 11:10 am
Coffee Break
11:10 am - 11:40 am
"Progress and Challenges in Electron Cryotomography"
11:40 am - 11:50 am
Discussion
11:50 am - 12:20 pm
"A Toolbox of Correlative Microscopy Approaches to Study Native Architectures of Cellular Membranes"
12:20 pm - 12:30 pm
Discussion
12:30 pm - 1:30 pm
Lunch
1:30 pm - 4:00 pm
Free Time
4:00 pm - 6:00 pm
Poster Session
6:00 pm - 7:00 pm
Dinner
7:30 pm - 9:30 pm
New Developments in Algorithms
7:30 pm - 7:45 pm
Introduction by Discussion Leader
7:45 pm - 8:10 pm
"Integrative Modelling of Macromolecular Assemblies"
8:10 pm - 8:20 pm
Discussion
8:20 pm - 8:45 pm
"A Particle-Filter Algorithm for Robust CryoEM 3D Reconstruction"
8:45 pm - 8:55 pm
Discussion
8:55 pm - 9:20 pm
"New Methods and Developments in CryoSPARC"
9:20 pm - 9:30 pm
Discussion
Thursday
7:30 am - 8:30 am
Breakfast
8:30 am - 9:00 am
Business Meeting
Nominations for the Next Vice Chair; Fill in Conference Evaluation Forms; Discuss Future Site and Scheduling Preferences; Election of the Next Vice Chair
9:00 am - 12:30 pm
Innovations in Hardware and Imaging
9:00 am - 9:20 am
Introduction by Discussion Leader
9:20 am - 9:50 am
"Laser-Based Phase Contrast"
9:50 am - 10:00 am
Discussion
10:00 am - 10:30 am
"Can We Expect Further Improvements in Detector Performance?"
10:30 am - 10:40 am
Discussion
10:40 am - 11:10 am
Coffee Break
11:10 am - 11:40 am
"Electron Optics Optimized for Cryo-EM"
11:40 am - 11:50 am
Discussion
11:50 am - 12:20 pm
"Resolution Advances in Cryo-EM"
12:20 pm - 12:30 pm
Discussion
12:30 pm - 1:30 pm
Lunch
1:30 pm - 4:00 pm
Free Time
4:00 pm - 6:00 pm
Poster Session
6:00 pm - 7:00 pm
Dinner
7:30 pm - 9:30 pm
Selected Poster Presentations: New Developments in 3DEM
7:30 pm - 7:40 pm
"Peripheral Myelin Protein 22 Alters Membrane Architecture"
7:40 pm - 7:45 pm
Discussion
7:45 pm - 7:55 pm
"A Cryo-EM Image Processing Workflow to Achieve Near-Atomic Resolution Reconstructions of Highly Flexible and Conformationally Dynamic Helical Filaments"
7:55 pm - 8:00 pm
Discussion
8:00 pm - 8:10 pm
"Sub-2 Å Ewald Curvature Corrected Single-Particle Cryo-EM"
8:10 pm - 8:15 pm
Discussion
8:15 pm - 8:25 pm
"StarMap: Rosetta Refinement Controlled from ChimeraX"
8:25 pm - 8:30 pm
Discussion
8:30 pm - 8:40 pm
"Next Generation Sample Preparation for Fully Automated Cryo-EM Analysis of Macromolecular Structures and Cells"
8:40 pm - 8:45 pm
Discussion
8:45 pm - 8:55 pm
"Enhanced Macromolecular Classification and Alignment for High Resolution Cryo-Electron Tomography"
8:55 pm - 9:00 pm
Discussion
9:00 pm - 9:10 pm
"In Situ Structural Biology Enabled by Integrating Cryo-EM with Mass Spectrometry"
9:10 pm - 9:15 pm
Discussion
9:15 pm - 9:25 pm
"No Phase Plate? No Problem!"
9:25 pm - 9:30 pm
Discussion
Friday
7:30 am - 8:30 am
Breakfast
9:00 am
Departure