Sunday
2:00 pm - 9:00 pm
Arrival and Check-in
6:00 pm - 7:00 pm
Dinner
7:30 pm - 7:40 pm
Introductory Comments by GRC Staff / Welcome and Introduction from the Chairs
7:40 pm - 9:30 pm
Keynote Session: 3DEM and Frontiers in Structural Biology
Discussion Leader: Bridget Carragher (Chan Zuckerberg Imaging Institute, United States)
7:40 pm - 7:45 pm
Opening Remarks
7:45 pm - 8:00 pm
Introduction by Discussion Leader
8:00 pm - 8:20 pm
Julia Mahamid (European Molecular Biology Laboratory, Germany)
"Enabling Discovery by In-Cell Structural Biology"
8:20 pm - 8:30 pm
Discussion
8:30 pm - 8:50 pm
Elizabeth Campbell (The Rockefeller University, United States)
"Structural Studies of Transcription in Bacterial and Viral Pathogens"
8:50 pm - 9:00 pm
Discussion
9:00 pm - 9:20 pm
Nigel Unwin (MRC Laboratory of Molecular Biology, United Kingdom)
"Structure of A Cell Membrane"
9:20 pm - 9:30 pm
Discussion
Monday
7:30 am - 8:30 am
Breakfast
9:00 am - 12:30 pm
The In Situ Revolution: Recent Breakthroughs and Innovations
Discussion Leader: Elizabeth Villa (University of California, San Diego and Howard Hughes Medical Institute, United States)
9:00 am - 9:15 am
Introduction by Discussion Leader
9:15 am - 9:35 am
Gaia Pigino (Fondazione Human Technopole, Italy)
"Structural Cell Biology of Cilia and Eukaryotic Flagella"
9:35 am - 9:50 am
Discussion
9:50 am - 10:10 am
Digvijay Singh (UCSD, United States)
"Structural Analysis of the Yeast Nuclear Pore Complex in situ "
10:10 am - 10:25 am
Discussion
10:25 am - 10:50 am
Coffee Break
10:50 am - 11:10 am
Bronwyn Lucas (University of California Berkeley, United States)
"Visualizing and Characterizing Single Particles in Cells with 2DTM"
11:10 am - 11:25 am
Discussion
11:25 am - 11:45 am
Benjamin Engel (Biozentrum, University of Basel, Switzerland)
"Exploring Photosynthesis and Carbon Fixation Across Scales with Cryo-Electron Tomography (EMBO Young Investigator Lecture)"
11:45 am - 12:00 pm
Discussion
12:00 pm - 12:20 pm
Zhen Chen (University of California, San Francisco, United States)
"High-Resolution In Situ Cryo-ET Enables Visual Proteomics of Mammalian Sperm"
12:20 pm - 12:30 pm
Discussion
12:30 pm - 1:30 pm
Lunch
1:30 pm - 4:00 pm
Free Time
4:00 pm - 6:00 pm
Poster Session
6:00 pm - 7:00 pm
Dinner
7:30 pm - 9:30 pm
Integrative and Correlative Approaches
Discussion Leader: Naoko Mizuno (NIH, United States)
7:30 pm - 7:45 pm
Introduction by Discussion Leader
7:45 pm - 8:05 pm
Jan Schuller (Phillips University Marburg, Germany)
"Single Particle and In-Situ Characterisation of Microbial Carbon Fixation and Energy Conservation"
8:05 pm - 8:20 pm
Discussion
8:20 pm - 8:40 pm
Wanda Kukulski (University of Bern, Switzerland)
"Discoveries in Cellular Architecture by Cryo-CLEM"
8:40 pm - 8:55 pm
Discussion
8:55 pm - 9:15 pm
Clemens Plaschka (IMP – Research Institute of Molecular Pathology, Austria)
"How To Recognize and Package Nuclear mRNA"
9:15 pm - 9:30 pm
Discussion
Tuesday
7:30 am - 8:30 am
Breakfast
9:00 am - 12:30 pm
Pushing Performance Limits with Challenging Samples
Discussion Leader: John Rubinstein (The Hospital for Sick Children, Canada)
9:00 am - 9:15 am
Introduction by Discussion Leader
9:15 am - 9:35 am
Claire Coupland (University of Oxford, United Kingdom)
"Using Megabodies to Study the Structure of HHAT, a Small, Integral Membrane Protein"
9:35 am - 9:50 am
Discussion
9:50 am - 10:10 am
Oliver Clarke (Columbia University, United States)
"Architecture and Organization of Erythrocyte Ankyrin Complexes"
10:10 am - 10:25 am
Discussion
10:25 am - 10:50 am
Coffee Break
10:50 am - 11:10 am
Alexey Amunts (Stockholm University, Sweden)
"Expanding the Frontiers of Cryo-EM Towards Bioenergetic Complexes"
11:10 am - 11:25 am
Discussion
11:25 am - 11:45 am
Yanyan Zhao (Stanford University, United States)
"CryoEM Visualization of Progressive Tubulin Folding Intermediates in Human Chaperonin TRiC"
11:45 am - 12:00 pm
Discussion
12:00 pm - 12:20 pm
Halil Aydin (University of Colorado Boulder, United States)
"Structural Mechanism of Mitochondrial Membrane Remodeling by Human OPA1"
12:20 pm - 12:30 pm
Discussion
12:30 pm - 1:30 pm
Lunch
1:30 pm - 4:00 pm
Free Time
4:00 pm - 6:00 pm
Poster Session
6:00 pm - 7:00 pm
Dinner
7:30 pm - 9:30 pm
Selected Poster Presentations: New Developments in 3DEM
Discussion Leader: Masahide Kikkawa (The University of Tokyo, Japan)
7:30 pm - 7:40 pm
Short Talk Selected from Poster Abstracts
7:40 pm - 7:45 pm
Discussion
7:45 pm - 7:55 pm
Short Talk Selected from Poster Abstracts
7:55 pm - 8:00 pm
Discussion
8:00 pm - 8:10 pm
Short Talk Selected from Poster Abstracts
8:10 pm - 8:15 pm
Discussion
8:15 pm - 8:25 pm
Short Talk Selected from Poster Abstracts
8:25 pm - 8:30 pm
Discussion
8:30 pm - 8:40 pm
Short Talk Selected from Poster Abstracts
8:40 pm - 8:45 pm
Discussion
8:45 pm - 8:55 pm
Short Talk Selected from Poster Abstracts
8:55 pm - 9:00 pm
Discussion
9:00 pm - 9:10 pm
Short Talk Selected from Poster Abstracts
9:10 pm - 9:15 pm
Discussion
9:15 pm - 9:25 pm
Short Talk Selected from Poster Abstracts
9:25 pm - 9:30 pm
Discussion
Wednesday
7:30 am - 8:30 am
Breakfast
8:30 am - 9:00 am
Group Photo
9:00 am - 12:30 pm
New Algorithms and AI Methods
Discussion Leader: Nikolaus Grigorieff (University of Massachusetts Chan Medical School, United States)
9:00 am - 9:15 am
Introduction by Discussion Leader
9:15 am - 9:35 am
Bertram Daum (Living Systems Institute, United Kingdom)
"The Symmetry Trap in Helical Reconstruction and How to Escape It"
9:35 am - 9:50 am
Discussion
9:50 am - 10:10 am
Slavica Jonic (CNRS & Sorbonne University, France)
"DeepHEMNMA: Extraction of Conformational Flexibility From cryo-EM Data by Combining Simulation, Image Analysis, and Deep Learning"
10:10 am - 10:25 am
Discussion
10:25 am - 10:50 am
Coffee Break
10:50 am - 11:10 am
Barrett Powell (MIT, United States)
"TomoDRGN: Exploring Structurally Heterogeneous Ribosomes in situ with Deep Learning"
11:10 am - 11:25 am
Discussion
11:25 am - 11:45 am
Kiarash Jamali (MRC Laboratory of Molecular Biology, United Kingdom)
"ModelAngelo: Automated Model Building and Protein Sequence Identification from Cryo-EM Maps"
11:45 am - 12:00 pm
Discussion
12:00 pm - 12:20 pm
Daisuke Kihara (Purdue University, United States)
"Building and Validating Biomolecular Structure Models for Cryo-EM Maps Using Deep Learning"
12:20 pm - 12:30 pm
Discussion
12:30 pm - 1:30 pm
Lunch
1:30 pm - 4:00 pm
Free Time
4:00 pm - 6:00 pm
Poster Session
6:00 pm - 7:00 pm
Dinner
7:30 pm - 9:30 pm
3DEM in Translational Research
Discussion Leader: Georgios Skiniotis (Stanford University, United States)
7:30 pm - 7:45 pm
Introduction by Discussion Leader
7:45 pm - 8:05 pm
Victoria Cushing (The Institute of Cancer Research, United Kingdom)
"Harnessing the Power of Cryo-EM for Discovery of Cancer Therapeutics"
8:05 pm - 8:20 pm
Discussion
8:20 pm - 8:40 pm
Xin Zhang (Monash University, Australia)
"Structural Insights into Class B1 G Protein-Coupled Receptor Activation and Allostery"
8:40 pm - 8:55 pm
Discussion
8:55 pm - 9:15 pm
Cornelius Gati (University of Southern California, United States)
"Structural Basis of GABA Reuptake Inhibition"
9:15 pm - 9:30 pm
Discussion
Thursday
7:30 am - 8:30 am
Breakfast
8:30 am - 9:00 am
Business Meeting
Nominations for the Next Vice Chair(s); Complete the GRC Evaluation Forms; Discuss Future Dates and Venue; Election of the Next Vice Chair(s)
9:00 am - 12:30 pm
Advances in Hardware and Imaging
Discussion Leader: Christopher Russo (MRC Laboratory of Molecular Biology, United Kingdom)
9:00 am - 9:15 am
Introduction by Discussion Leader
9:15 am - 9:35 am
Priya Ramakrishna (EPFL, Lausanne, Switzerland, Switzerland)
"Correlated Cryo-SEM and CryoNanoSIMS Imaging of Biological Tissue"
9:35 am - 9:50 am
Discussion
9:50 am - 10:10 am
Anthony Fitzpatrick (Columbia University, United States)
"Design and Implementation of an Ultrafast Pulsed Ponderomotive Phase Plate for Cryo-Electron Tomography"
10:10 am - 10:25 am
Discussion
10:25 am - 10:50 am
Coffee Break
10:50 am - 11:10 am
Juergen Plitzko (Max Planck Institute of Biochemistry, Germany)
"Next Generation Sample Preparation for Cryo-ET - Instruments, Accessories and Workflows"
11:10 am - 11:25 am
Discussion
11:25 am - 11:45 am
Radu Aricescu (MRC Laboratory for Molecular Biology, United Kingdom)
"Expansion Microscopy for Structural Biology"
11:45 am - 12:00 pm
Discussion
12:00 pm - 12:20 pm
Peiyi Wang (Southern University of Science and Technology, China)
"Chromatic Aberration Corrector for Cryo-EM and Its Application in the Structural Biology"
12:20 pm - 12:30 pm
Discussion
12:30 pm - 1:30 pm
Lunch
1:30 pm - 4:00 pm
Free Time
4:00 pm - 6:00 pm
Poster Session
6:00 pm - 7:00 pm
Dinner
7:30 pm - 9:30 pm
Selected Poster Presentations: New Developments in 3DEM
Discussion Leader: Michael Cianfrocco (University of Michigan, United States)
7:30 pm - 7:40 pm
Short Talk Selected from Poster Abstracts
7:40 pm - 7:45 pm
Discussion
7:45 pm - 7:55 pm
Short Talk Selected from Poster Abstracts
7:55 pm - 8:00 pm
Discussion
8:00 pm - 8:10 pm
Short Talk Selected from Poster Abstracts
8:10 pm - 8:15 pm
Discussion
8:15 pm - 8:25 pm
Short Talk Selected from Poster Abstracts
8:25 pm - 8:30 pm
Discussion
8:30 pm - 8:40 pm
Short Talk Selected from Poster Abstracts
8:40 pm - 8:45 pm
Discussion
8:45 pm - 8:55 pm
Short Talk Selected from Poster Abstracts
8:55 pm - 9:00 pm
Discussion
9:00 pm - 9:10 pm
Short Talk Selected from Poster Abstracts
9:10 pm - 9:15 pm
Discussion
9:15 pm - 9:25 pm
Short Talk Selected from Poster Abstracts
9:25 pm - 9:30 pm
Discussion
Friday
7:30 am - 8:30 am
Breakfast
9:00 am
Departure