Sunday
2:00 pm - 9:00 pm
Arrival and Check-in
6:00 pm - 7:00 pm
Dinner
7:30 pm - 7:40 pm
Introductory Comments by GRC Staff / Welcome and Introduction from the Chairs
7:40 pm - 9:30 pm
Keynote Session: Key vEM Technologies
Discussion Leader: Yannick Schwab (European Molecular Biology Laboratory, Germany)
7:40 pm - 8:10 pm
C. Shan Xu (Yale School of Medicine, United States)
"Visualizing Biology Across Scales using Enhanced FIB-SEM"
8:10 pm - 8:15 pm
Discussion
8:15 pm - 8:45 pm
christel Genoud (Université de Lausanne, Switzerland)
"Volume Electron Microscopy on a Platform : From Large Volume to Cryo-NanoSIMs"
8:45 pm - 8:50 pm
Discussion
8:50 pm - 9:20 pm
Jeff Lichtman (Harvard University, United States)
"Multi-Cubic Millimeter Volumes for Electronmicroscopy-Based Connectomics of Human Brain Biopsies"
9:20 pm - 9:25 pm
Discussion
9:25 pm - 9:30 pm
General Discussion
Monday
7:30 am - 8:30 am
Breakfast
8:30 am - 9:00 am
Group Photo
9:00 am - 12:30 pm
Key Applications of vEM
Discussion Leader: Saskia Lippens (VIB, Belgium)
9:00 am - 9:05 am
Introduction by Discussion Leader
9:05 am - 9:35 am
Jost Enninga (Institut Pasteur, France)
"Combining Multiple CLEM Workflows to Decipher the Intracellular Niche Formation of Entero-Invasive Bacteria in 3D"
9:35 am - 9:45 am
Discussion
9:45 am - 10:15 am
Claudia Lopez (Oregon Health & Science University, United States)
"Elucidation of Candidate Functional Nano Architectures in Metastatic Cancer Using vEM."
10:15 am - 10:25 am
Discussion
10:25 am - 11:00 am
Coffee Break
11:00 am - 11:30 am
Moritz Helmstaedter (Max Planck Institute for Brain Research, Germany)
"Cerebral Cortex Connectomics"
11:30 am - 11:40 am
Discussion
11:40 am - 11:55 am
Short Talk Selected from Poster Abstracts
11:55 am - 12:00 pm
Discussion
12:00 pm - 12:15 pm
Short Talk Selected from Poster Abstracts
12:15 pm - 12:20 pm
Discussion
12:20 pm - 12:30 pm
General Discussion
12:30 pm - 1:30 pm
Lunch
1:30 pm - 4:00 pm
Free Time
3:00 pm - 4:00 pm
The GRC Power Hour™
The GRC Power Hour™ is designed to address diversity and inclusion in the scientific workplace by providing a safe environment for informal and meaningful conversations amongst colleagues of all career stages. The program supports the professional growth of all members of our communities, including ethnicity, race and/or gender identity by providing an open forum for discussion and mentoring.
Organizers: Nalan Liv (University Medical Center Utrecht, The Netherlands), Erin Tranfield (Instituto Gulbenkian de Ciência, Portugal) and Song Pang (Yale School of Medicine, United States)
4:00 pm - 6:00 pm
Poster Session
6:00 pm - 7:00 pm
Dinner
7:30 pm - 9:30 pm
Tackling Sample Preparation Challenges for vEM
Discussion Leader: Nicole L. Schieber (Electron Microscopy for Life Sciences and Soft Matter Laboratory, Australia)
7:30 pm - 7:35 pm
Introduction by Discussion Leader
7:35 pm - 8:05 pm
Thomas Templier (Collectome, Switzerland)
"How to Magnetically Collect Tissue Sections and What To Do With Them"
8:05 pm - 8:15 pm
Discussion
8:15 pm - 8:25 pm
Short Talk Selected from Poster Abstracts
8:25 pm - 8:30 pm
Discussion
8:30 pm - 9:00 pm
Emily Benson (Cleveland Clinic, United States)
"Automated Sample Preparation for Pre-Clinical and Niche Applications of Volume Electron Microscopy"
9:00 pm - 9:10 pm
Discussion
9:10 pm - 9:20 pm
Short Talk Selected from Poster Abstracts
9:20 pm - 9:25 pm
Discussion
9:25 pm - 9:30 pm
General Discussion
Tuesday
7:30 am - 8:30 am
Breakfast
9:00 am - 12:30 pm
Molecular Labelling Strategies for vEM
Discussion Leader: Ben Giepmans (UMC Groningen, The Netherlands)
9:00 am - 9:05 am
Introduction by Discussion Leader
9:05 am - 9:35 am
Kristina Micheva (Stanford University, United States)
"Immuno-vEM"
9:35 am - 9:45 am
Discussion
9:45 am - 10:15 am
Kara Fulton (Harvard Medical School, United States)
"Correlative Light and Electron Microscopy for Mapping Cell Type-Specific Connectivity"
10:15 am - 10:25 am
Discussion
10:25 am - 11:00 am
Coffee Break
11:00 am - 11:25 am
Jemima Burden (University College London, United Kingdom)
"Array Tomography - A Flexible Volume EM Technique"
11:25 am - 11:35 am
Discussion
11:35 am - 11:50 am
Short Talk Selected from Poster Abstracts
11:50 am - 11:55 am
Discussion
11:55 am - 12:10 pm
Short Talk Selected from Poster Abstracts
12:10 pm - 12:15 pm
Discussion
12:15 pm - 12:30 pm
General Discussion
12:30 pm - 1:30 pm
Lunch
1:30 pm - 4:00 pm
Free Time
4:00 pm - 6:00 pm
Poster Session
6:00 pm - 7:00 pm
Dinner
7:30 pm - 9:30 pm
High-Speed, High-Volume vEM Imaging
Discussion Leader: Nalan Liv (University Medical Center Utrecht, The Netherlands)
7:30 pm - 8:00 pm
Alex de Marco (NYSBC, United States)
"Increasing Acquisition Speed and Volume in FIB/SEM Tomography"
8:00 pm - 8:10 pm
Discussion
8:10 pm - 8:40 pm
Meike Sievers (Max Planck Institute for Brain Research, Germany)
"Large-Scale Connectomics with Multibeam Scanning Electron Microscopy"
8:40 pm - 8:50 pm
Discussion
8:50 pm - 9:20 pm
Benjamin Campbell (UCSD, United States)
"Enhancing the Chemical Stability of Fluorescent Proteins for Advanced Microscopy"
9:20 pm - 9:30 pm
Discussion
Wednesday
7:30 am - 8:30 am
Breakfast
9:00 am - 12:30 pm
Tools for Acquiring, Processing and Analysing vEM Data
Discussion Leader: Erin Tranfield (Instituto Gulbenkian de Ciência, Portugal)
9:00 am - 9:05 am
Introduction by Discussion Leader
9:05 am - 9:35 am
John Bogovic (HHMI Janelia, United States)
"Toward Accessible, Scalable, and Interoperable Image Registration with BigWarp"
9:35 am - 9:45 am
Discussion
9:45 am - 10:00 am
Short Talk Selected from Poster Abstracts
10:00 am - 10:05 am
Discussion
10:05 am - 10:20 am
Short Talk Selected from Poster Abstracts
10:20 am - 10:25 am
Discussion
10:25 am - 11:00 am
Coffee Break
11:00 am - 11:30 am
Anna Kreshuk (European Molecular Biology Laboratory, Germany)
"Unbiased Exploration of Cells, Tissues and Organs in VolumeEM"
11:30 am - 11:40 am
Discussion
11:40 am - 11:55 am
Short Talk Selected from Poster Abstracts
11:55 am - 12:00 pm
Discussion
12:00 pm - 12:15 pm
Short Talk Selected from Poster Abstracts
12:15 pm - 12:20 pm
Discussion
12:20 pm - 12:30 pm
General Discussion
12:30 pm - 1:30 pm
Lunch
1:30 pm - 4:00 pm
Free Time
4:00 pm - 6:00 pm
Poster Session
6:00 pm - 7:00 pm
Dinner
7:30 pm - 9:30 pm
Visualising and Sharing Big vEM Data
Discussion Leader: Aubrey Weigel (Janelia Research Campus, Howard Hughes Medical Institute, United States)
7:30 pm - 8:00 pm
Gerard Kleywegt (EMBL-EBI, United Kingdom)
"Public Archiving of vEM Data"
8:00 pm - 8:10 pm
Discussion
8:10 pm - 8:40 pm
Joshua Moore (OME / German BioImaging e.V., Germany)
"OME-Zarr: A Cloud-Optimized Bioimaging File Format with International Community Support"
8:40 pm - 8:50 pm
Discussion
8:50 pm - 9:20 pm
Christian Tischer (EMBL, Germany)
"Correlative Big Image Data Visualisation Using Open Source Software and Community Standards"
9:20 pm - 9:30 pm
Discussion
Thursday
7:30 am - 8:30 am
Breakfast
8:30 am - 9:00 am
Business Meeting
Nominations for the Next Vice Chair(s); Complete the GRC Evaluation Forms; Discuss Future Dates and Venue; Election of the Next Vice Chair(s)
9:00 am - 12:30 pm
Volume Correlative Microscopy
Discussion Leader: Kedar Narayan (Frederick National Laboratory, National Cancer Institute, NIH, United States)
9:00 am - 9:05 am
Introduction by Discussion Leader
9:05 am - 9:35 am
Eija Jokitalo (University of Helsinki, Finland)
"Three Reasons to Correlate in VolumeEM"
9:35 am - 9:45 am
Discussion
9:45 am - 10:00 am
Short Talk Selected from Poster Abstracts
10:00 am - 10:05 am
Discussion
10:05 am - 10:20 am
Short Talk Selected from Poster Abstracts
10:20 am - 10:25 am
Discussion
10:25 am - 11:00 am
Coffee Break
11:00 am - 11:30 am
Kimberly Meechan (European Molecular Biology Laboratory (EMBL), Germany)
"Targeted Volume EM using X-Ray Imaging and a Motorised Ultramicrotome"
11:30 am - 11:40 am
Discussion
11:40 am - 11:55 am
Short Talk Selected from Poster Abstracts
11:55 am - 12:00 pm
Discussion
12:00 pm - 12:15 pm
Short Talk Selected from Poster Abstracts
12:15 pm - 12:20 pm
Discussion
12:20 pm - 12:30 pm
General Discussion
12:30 pm - 1:30 pm
Lunch
1:30 pm - 4:00 pm
Free Time
4:00 pm - 6:00 pm
Poster Session
6:00 pm - 7:00 pm
Dinner
7:30 pm - 9:30 pm
Latest Developments in the vEM Field
Discussion Leaders: Song Pang (Yale School of Medicine, United States)
7:30 pm - 7:35 pm
Introduction by Discussion Leader
7:35 pm - 8:05 pm
Jacob Hoogenboom (Delft University of Technology, The Netherlands)
"Faster SEM for Volume Imaging"
8:05 pm - 8:10 pm
Discussion
8:10 pm - 8:40 pm
Alexandra Pacureanu (ESRF - the European Synchrotron, France)
"X-Ray Multiscale 3D Neuroimaging to Quantify Cellular Aging and Neurodegeneration"
8:40 pm - 8:45 pm
Discussion
8:45 pm - 9:15 pm
Rob Parton (University of Queensland, Australia)
"Cave Exploration at the Nanoscale: Multimodal Molecular Imaging to Study Membrane Microdomains"
9:15 pm - 9:20 pm
Discussion
9:20 pm - 9:30 pm
Closing Remarks
Friday
7:30 am - 8:30 am
Breakfast
9:00 am
Departure