| Sunday Evening, June 25 |
| 7:30-7:45 PM |
Welcome and Opening Remarks: Juan S. Bonifacino |
| Session 1: |
The Structure of the Endocytic Machinery |
| Discussion Leader: Frances Brodsky |
| 7:45-7:50 PM |
Introduction |
| 7:50-8:15 |
Frances Brodsky (UCSF, San Francisco, CA)
Biological basketweaving: clathrin-coated vesicle formation. |
| 8:25-8:50 |
Tomas Kirchhausen (Harvard Medical School, Boston, MA)
Molecular view of the clathrin endocytic machinery |
| 9:00-9:25 |
David Owen (MRC, Cambridge, UK)
Structure and function of components of adaptor complexes |
| 9:35-9:50 |
Nancy Dahms (Medical College of Wisconsin, Milwaukee, WI)
Crystal structure of the cation-dependent mannose 6-phosphate receptor |
| 10:00- |
Reception at the old chapel |
| Monday, June 26 |
| 8:30 AM |
Group Photo (hillside on Farrell Field) |
| Session 2: |
Mechanisms of Endocytosis |
| Discussion Leader: Linda Hicke |
| 9:00-9:05 AM |
Introduction |
| 9:05-9:30 |
Pietro de Camilli (Yale Medical School, New Haven, CT)
Endocytosis at the synapse and the role of regulatory molecules |
| 9:40-9:50 |
Beverly Wendland (Johns Hopkins University, Baltimore, MD)
Evidence linking the yeast epsin homologue Ent1p to Ark protein kinases |
| 9:55-10:20 |
Sandra Schmid (The Scripps Research Institute, La Jolla, CA)
Probing dynamin function in vivo |
| 10:30-10:50 |
Break |
| 10:50-11:00 |
Julie Donaldson (NIH, Bethesda, MD)
ARF6-dependent membrane trafficking and the spatial control of PIP2 synthesis |
| 11:05-11:15 |
Mark McNiven (Mayo Clinic, Rochester, MN)
Functional Interactions between dynamin and the actin cytoskeleton |
| 11:20-:11:45 |
Sandra Lemmon (Case Western Reserve University, Cleveland, OH)
Regulation of endocytosis and the actin cytoskeleton by a novel suppressor of clathrin deficiency |
| 11:55-12:20 |
Linda Hicke (Northwestern University, Chicago, IL)
Role of ubiquitin and ubiquitination machinery in receptor internalization |
| 4:00-6:00 |
Poster Session I |
| Session 3: |
Transport and Sorting in the Endocytic Pathway |
| Discussion Leader: Fred Maxfield |
| 7:30-7:35 PM |
Introduction |
| 7:35-8:00 |
Ann Hubbard (Johns Hopkins University, Baltimore, MD)
PI-3 kinases and dynamics of apical proteins in hepatocytes |
| 8:10-8:35 |
Fred Maxfield (Weill Medical College, New York, NY)
Direct observations of sterols and lipid domains in living cells |
| 8:45-9:00 |
Break |
| 9:00-9:10 |
Suzanne Pfeffer (Stanford University, CA)
Rab9 and TIP47 in mannose 6-phosphate receptor trafficking |
| 9:15-9:40 |
Helmut Krämer (UTSW, Dallas, TX)
Endocytic trafficking in Drosophila |
| 9:50-10:00 |
Thierry Soldati (Max-Planck Institute, Heidelberg, Germany)
Myosin I and plasma membrane recycling |
| Tuesday, June 27 |
| Session 4: |
Vesicle Budding and Fusion |
| Discussion Leader: Bill Wickner |
| 9:00-9:05 AM |
Introduction |
| 9:05-9:30 |
Silvia Corvera (University of Massachussetts, Worcester MA)
Analysis of endosome fusion using high speed digital imaging microscopy |
| 9:40-9:50 |
Bruce Horazdovsky (UTSW, Dallas, TX)
EGF endocytosis is mediated by the Rab5 nucleotide exchange activity of Rin1
|
| 9:55-10:05 |
Valeria Cavalli (University of Geneva, Switzerland)
The stress-induced MAP kinase p38 regulates endocytic trafficking via the GDI:Rab5 complex |
| 10:10-10:20 |
Todd Graham (Vanderbilt University, Nashville, TN)
Interaction between Drs2p and an ARF-GEF: Implications for clathrin recruitment to the TGN
|
| 10:25-10:50 |
Break |
| 10:50-11:15 |
Richard Scheller (Stanford University, Stanford CA)
Molecular mechanisms of membrane trafficking and exocytosis |
| 11:25-11:50 |
Bill Wickner (Dartmouth Medical School, Hanover NH)
Mechanisms of vacuole fusion |
| 12:00-12:15 |
Andreas Mayer (Max Planck Institute, Tübingen, Germany)
Membrane invagination and budding into the lumen of yeast vacuoles |
| 4:00-6:00 |
Poster Session I (Continued) |
| Session 5: |
Protein Sorting at the TGN |
| Discussion Leader: Jennifer Lippincott-Schwartz |
| 7:30-7:35 PM |
Introduction |
| 7:35-8:00 |
Jennifer Lippincott-Schwartz (NIH, Bethesda MD)
A membrane cycling pathway between the plasma membrane and the Golgi complex followed by GPI-anchored proteins and bacterial toxins |
| 8:10-8:35 |
Peter Arvan (Albert Einstein College of Medicine, Bronx NY)
Luminal protein sorting in the TGN and immature secretory granules |
| 8:45-9:00 |
Break |
| 9:00-9:10 |
Ann Erickson (University of North Carolina, Chapel Hill, NC)
Alternate targeting pathways for lysosomal proteases in fibroblasts |
| 9:15-9:25 |
Esteban Dell'Angelica (UCLA, Los Angeles, CA)
The GGA family defines a novel protein module that mediates ARF-dependent targeting to the Golgi complex |
| 9:30-9:55 |
Greg Payne (UCLA, Los Angeles, CA)
Clathrin-mediated protein transport from the yeast TGN. |
| Wednesday, June 28 |
| Session 6: |
Endosomes and Lysosomes in Cell Physiology |
| Discussion Leader: Norma Andrews |
| 9:00-9:05 AM |
Introduction |
| 9:05-9:30 |
Hans Geuze (University of Utrecht, The Netherlands)
Maturing dendritic cells remodel their endocytic compartments to facilitate antigen-loading and transport of MHC class II to the cell surface |
| 9:40-10:05 |
Thomas Willnow (Max Delbrück Center, Berlin, Germany)
Receptor-mediated uptake of steroids and retinoids |
| 10:15-10:25 |
John Incardona (University of Washington, Seattle, WA)
Functional late endosomes are required for Sonic hedgehog signal transduction |
| 10:30-10:50 |
Break |
| 10:50-11:15 |
Norma Andrews (Yale Medical School, New Haven CT)
Ca2+-regulated exocytosis of conventional lysosomes |
| 11:25-11:50 |
Paul McNeil (Medical College of Georgia, Augusta GA)
Role of endosomes and lysosomes in restoration and maintenance of plasma membrane machinery |
| 12:00-12:10 |
Vojo Deretic (University of Michigan, Ann Arbor, MI)
Phagosomal maturation and block by Mycobacterium tuberculosis |
| 4:00-6:00 |
Poster Session II |
| 7:15 PM |
Business Meeting: Election of new Vice-chair for the year 2002 and Chair for the year 2004 |
| Session 7: |
The Yeast Vacuole |
| Discussion Leader: Scott Emr |
| 7:30-7:35 PM |
Introduction |
| 7:35-8:00 |
Liz Conibear (University of Oregon, Eugene, OR)
A novel sorting complex required for retrograde transport to the yeast late Golgi |
| 8:10-8:35 |
Markus Babst (UCSD, San Diego, CA)
Membrane associated Vps pProteins required for endosomal sorting
|
| 8:45-9:00 |
Break |
| 9:00-9:25 |
Lois Weisman (University of Iowa, Iowa City IA)
Regulation of membrane movement and fusion during yeast vacuole inheritance. |
| 9:35-10:00 |
Dan Klionsky (UC, Davis CA)
The cytoplasm to vacuole targeting pathway in yeast |
| Thursday, June 29 |
| Session 8: |
Disorders of Lysosome Biogenesis and Function |
| Discussion Leader: Alessandra d'Azzo |
| 9:00-9:05 AM |
Chair's Introduction |
| 9:05-9:30 |
Peter Lobel (Rutgers, New Brunswick NY)
Lysosomal enzyme trafficking on the road to disease discovery: molecular basis for late infantile neuronal ceroid lipofuscinosis |
| 9:40-10:05 |
Alessandra d'Azzo (St Jude's Children's Hospital, Memphis, TN)
Lysosomal disorders and the serendipity of overlapping transcriptional units |
| 10:15-10:25 |
Michael Marks (University of Pennsylvania, Philadelphia, PA)
Melanosome biogenesis: relationship of melanosomes to the endocytic pathway |
| 10:30-10:50 |
Break |
| 10:50-11:15 |
Jerry Kaplan (University of Utah, Salt Lake City, UT)
Genetic and biochemical analysis of Chediak-Higashi syndrome |
| 11:25-11:50 |
Richard Swank (Roswell Park Cancer Institute, Buffalo, NY)
Mouse models of Hermansky-Pudlak syndrome |
| 12:00-12:10 |
Vittoria Schiaffino (Telethon Institute of Genetics and Medicine, Milan, Italy)
Ocular albinism: a defect in an intracellular signal transduction system |
| 4:00-6:00 |
Poster Session II (Continued) |
| 6:00-7:30 PM |
Banquet |
| 8:00-8:10 |
Introduction to the Alex Novikoff Lecture |
| 8:10-9:10 |
Alex Novikoff Lecture: Ira Mellman (Yale Medical School, New Haven, CT)
Antigen presentation for amateurs and professionals |
| 9:10-10:00 |
Current Trends in Lysosome Research: An Open Forum
Discussion Leaders: Juan S. Bonifacino, Scott Emr, Vice Chair Elect |
| 10:00- |
Reception at the old chapel |
