| SUNDAY |
| 2:00-9:00 pm | Arrival and Check-in |
| 5:00-6:00 pm | Welcome Reception |
| 6:00 - 7:00 pm | Dinner |
| 7:30-7:40 pm | Steven S. Gross: Welcome and Introductory Remarks |
| 7:30 - 9:30 | Prokaryotic NOS-like Proteins |
| Moderator: Bernd Mayer, Unversity of Graz |
| 7:40-8:20 pm | Brian Crane and Rosemary Loria, Cornell University, Ithaca, NY.
Prokaryotic NOSs: Structure and Emerging Functions. |
| 8:20-8:40 pm | Discussion |
| 8:40-9:05 pm | C.S. Raman, University of Texas at Houston, TX.
Structure and Function of NOS-like Proteins from Bacterial Pathogens. |
| 9:05-9:25 pm | Discussion |
| MONDAY |
| 7:30 - 8:30 am | Breakfast |
| 9:00 am - 12:30 pm | NOS Structure, Function and Control |
| Moderator: Dennis Stuehr, Cleveland Clinic |
| 9:00 - 9:25 am | Libby Getzoff, Scripps Research Institute, La Jolla CA.
The nNOS Reductase Domain: Structural Insights into the Route of Electron Transfer. |
| 9:25 - 9:40 am | Discussion |
| 9:40 - 10:05 am | Simon Daff, University of Edinburgh, UK.
Regulation of Electron Flux Through nNOS by Calmodulin Binding. |
| 10:05 -10:20 am | Discussion |
| 10:20 - 10:45 am | Bettie Sue Masters, University of Texas Health Science Center at San Antonio, TX.
Autoinhibitory Peptide Control Elements in the Regulation of cNOSs. |
| 10:45 -11:00 am | Discussion |
| 11:00 - 11:45 am | Coffee Break and Group Photo |
| 11:45 - 12:10 pm | Bernd Mayer, University of Graz, Austria.
Towards a Global Understanding of the NOS Catalytic Mechanism. |
| 12:10 - 12:30 pm | Discussion |
| 12:30-1:30 pm | Lunch |
| Afternoon is open for discussion |
| 4:30-6:00 pm | Poster Session |
| 6:00 pm | Dinner |
| 7:30 - 9:30 pm | The Active Site of NOS |
| Moderator: Owen W. Griffith, Medical College of Wisconsin |
| 7:30-7:55 pm | Peng George Wang, Wayne State University, Detroit MI.
Novel NOS Substrates. |
| 7:55-8:10 pm | Discussion |
| 8:10-8:35 pm | Tom Poulos, Univ. Cal-Irvine, Irvine CA
Towards a Structural Basis for How Isoform-Selective NOS Inhibitors Work |
| 8:35-8:50 pm | Discussion |
| 8:50-9:15 pm | Denis Rousseau, Albert Einstein College of Medicine, Bronx NY.
Protein/Substrate Interactions That Define the NOS Heme Environment. |
| 9:15-9:30 pm | Discussion |
| TUESDAY |
| 7:30 - 8:30 am | Breakfast |
| 9:00 am - 12:30 pm | NOS-Containing Signaling Modules: Structural Basis and Functional Consequences |
| Moderator: Richard C. Venema, Medical College of
Georgia |
| 9:00-9:25 am | William C. Sessa, Yale University School of Medicine, New Haven CT.
Structural Basis and Mechanistic Consequences of eNOS Targeting to Caveolae. |
| 9:25-9:45 am | Discussion |
| 9:45-10:10 am | Joshua M. Hare, Johns Hopkins Hospital, Baltimore MD.
Spatial Confinement of NOS Isoforms in Myocardium: Opposing Actions of NO Arise From Production at Discrete subcellular Loci. |
| 10:10-10:30 am | Discussion |
| 10:30-11:00 am | Coffee Break |
| 11:00-11:25 am | Cicilia Giulivi, University of Minnesota, MN.
Mitochondrial NOS and Mitochondrial Protein Nitration. |
| 11:25-11:45 am | Discussion |
| 11:45-12:10 pm | Vijay Shah, Mayo Clinic, Rochester MN
Interaction of eNOS with Dynamin-2 |
| 12:10-12:30 pm | Discussion |
| 12:30-1:30 pm | Lunch |
| Afternoon is open for discussion |
| 4:30-6:00 pm | Poster Session |
| 6:00 pm | Dinner |
| 7:30 - 9:30 pm | Posttranslational Mechanisms of eNOS Regulation in Health and Disease |
| Moderator: William C. Sessa, Yale University School of Medicine |
| 7:30-7:55 pm | Richard Venema, Medical College of Georgia, Augusta GA.
Differential Phosphorylation of eNOS at Multiple Regulatory Sites for Control of Enzymatic Activity. |
| 7:55-8:10 pm | Discussion |
| 8:10-8:35 p.m | Arthur L. Burnett, Johns Hopkins University School of Medicine, Baltimore MD.
Akt-Mediated eNOS Phosphorylation and Penile Erection. |
| 8:35-8:50 pm | Discussion |
| 8:50-9:15 pm | Michael Brownlee, Albert Einstein College of Medicine, Bronx NY.
Hyperglycemia Inhibits Endothelial Nitric Oxide Synthase Activity by Posttranslational Modification at the Akt site. |
| 9:15-9:30 pm | Discussion |
| WEDNESDAY |
| 7:30-8:30 am | Breakfast |
| 9:00 am - 12:30 pm | In Vivo Chemistry and Metabolism of NO and Related Molecules |
| Moderator: John Fukuto, UCLA |
| 9:00-9:25 am | Jonathan S. Stamler, Howard Hughs Medical Institute, Duke University Medical Ctr., Durham, NC.
Metabolism of Nitroglycerine to NO. |
| 9:25-9:45 am | Discussion |
| 9:45-10:10 am | Kendall N. Houk, UCLA School of Medicine, Los Angeles, CA.
Theoretical Studies of the Formation and Reactions of NO, HNO and RSNOs |
| 10:10-10:30 am | Discussion |
| 10:30-11:00 am | Coffee Break |
| 11:00-11:25 am | Bulent Mutus, University of Windsor, Ontario, Canada.
Mechanism of NO Transfer to Cytosol From Extracellular SNO-Proteins. |
| 11:25-11:45 am | Discussion |
| 11:45-12:10 pm | Kulwant Aulak, Cleveland Clinic Foundation, Cleveland, OH.
Potential Relevance and Targets of Protein Nitration In Vivo |
| 12:10-12:30 pm | Discussion |
| 12:30-1:30 pm | Lunch |
| Afternoon is open for discussion |
| 4:30-6:00 pm | Poster Session |
| 6:00 - 7:00 pm | Dinner |
| 7:00 - 7:40 pm | Business Meeting |
| 7:40 - 9:00 pm | Hot Topics |
| TBA |
| THURSDAY |
| 7:30 - 8:30 am | Breakfast |
| 9:00 am- 12:30 pm | Nitric Oxide and Respiration: Multiple Levels of Control |
| Moderator: Jonathan Stamler, HHMI Duke University Medical Center |
| 9:00-9:25 am | Maurizio Brunori, Università de Roma, 'La Sapienza', Italy.
Regulation of Cellular Respiration: Mechanisms and Controversies. |
| 9:25-9:45 am | Discussion |
| 9:45-10:10 am | Timothy McMahon, Duke University Medical Center, Durham, NC.
NO in the Human Respiratory Cycle. |
| 10:10-10:30 am | Discussion |
| 10:30-11:00 am | Coffee Break |
| 11:00-11:25 am | Benjamin Gaston, University of Virginia Health Science Ctr., Charlottesville, VA.
S-Nitrosothiols Signal the Ventilatory Response to Hypoxia. |
| 11:25-11:45 am | Discussion |
| 11:45-12:10 pm | Cynthia M. Beall, Case Western Reserve, Cleveland OH.
Nitric oxide Mediates Pulmonary Adaptation to High-Altitude. |
| 12:10-12:30 pm | Discussion |
| 12:30-1:30 pm | Lunch |
| Afternoon is open for discussion |
| 4:30-6:00 pm | Poster Session |
| 6:00 - 7:00 pm | Dinner |
| 7:30 - 9:30 pm | Nitric Oxide Signaling in Disease |
| Moderator: Pamela Manning, Pharmacia |
| 7:30-7:55 pm | David Wink, National Cancer Institute, Frederick MD. Oxidative vs. Nitrosative Stress Elicited by NO In Vivo: Stressful or Regulatory. |
| 7:55-8:10 pm | Discussion |
| 8:10-8:35 pm | Joseph S. Beckman, Linus Pauling Institute, Oregon Univ., Corvalis OR.
NO, Peroxynitrite and Motor Neuron Death. |
| 8:35-8:50 pm | Discussion |
| 8:50-9:15 pm | Arthur H. Neufeld, Washington University School of Medicine, St. Louis, MO.
NO and Glaucomatous Optic Neuropathy |
| 9:15-9:30 pm | Discussion |
| FRIDAY |
| 7:30 - 8:30 am | Breakfast |
| 9:00 am | Departure |
