Sunday
2:00 pm - 9:00 pm
Arrival and Check-in
6:00 pm - 7:00 pm
Dinner
7:30 pm - 7:40 pm
Introductory Comments by GRC Staff / Welcome and Introduction from the Chairs
7:40 pm - 9:30 pm
Towards a Plant Cell Atlas
7:40 pm - 7:50 pm
Introduction by Discussion Leader
7:50 pm - 8:10 pm
"Who is Talking to Whom? Genome-Wide Cell-Cell Communication via the Symplast"
8:10 pm - 8:20 pm
Discussion
8:20 pm - 8:30 pm
"Evolution of Development of Barrier Cell Types in Legume Roots"
8:30 pm - 8:35 pm
Discussion
8:35 pm - 8:55 pm
"Understanding the Diversity, Function, and Regulation of Plant Immune Cell States"
8:55 pm - 9:05 pm
Discussion
9:05 pm - 9:15 pm
"The Subtle Art of Plant Defense: A Single-Cell Perspective on Beneficial Microbe-Induced Immunity Priming"
9:15 pm - 9:20 pm
Discussion
9:20 pm - 9:30 pm
General Discussion
Monday
7:30 am - 8:30 am
Breakfast
9:00 am - 12:30 pm
Technical Challenges and Opportunities in Single Cell Approaches
9:00 am - 9:10 am
Introduction by Discussion Leader
9:10 am - 9:30 am
"Getting to the Root of Cell Fate Decisions"
9:30 am - 9:40 am
Discussion
9:40 am - 10:00 am
"Single Cell Profiling of Shoot Stem Cells Identifies Novel Conserved Meristem Regulators"
10:00 am - 10:10 am
Discussion
10:10 am - 10:25 am
"An Antagonistic Epigenetic Mechanism Regulating Gene Expression in Pollen Revealed Through Single-Nucleus Multiomics"
10:25 am - 10:30 am
Discussion
10:30 am - 11:00 am
Coffee Break
11:00 am - 11:20 am
"Challenges and Opportunities for Plant Mass Spectrometry Imaging at Single Cell Resolution"
11:20 am - 11:30 am
Discussion
11:30 am - 11:45 am
"Using Single-Cell Genomics to Explore Transcriptional Divergence and Cis-Regulatory Dynamics of Duplicated Genes"
11:45 am - 11:50 am
Discussion
11:50 am - 12:05 pm
"Improving scRNA-seq Quantification in Plants Through Refined 3' UTR Annotation"
12:05 pm - 12:10 pm
Discussion
12:10 pm - 12:20 pm
General Discussion
12:20 pm - 12:30 pm
Poster Previews
12:30 pm - 1:30 pm
Lunch
1:30 pm - 4:00 pm
Free Time
4:00 pm - 6:00 pm
Poster Session
6:00 pm - 7:00 pm
Dinner
7:30 pm - 9:30 pm
Systems and Synthetic Biology Approaches in Single Cell Biology
7:30 pm - 7:40 pm
Introduction by Discussion Leader
7:40 pm - 8:00 pm
"New Methods for Targeted DNA Insertion in Plants"
8:00 pm - 8:10 pm
Discussion
8:10 pm - 8:25 pm
"Development of Pooled Gene Perturbation and Protoplast Sorting Technologies Enable a First-In-Class, in planta Single-Cell Genetic Screen"
8:25 pm - 8:30 pm
Discussion
8:30 pm - 8:50 pm
"Using Ecologically and Phylogenetically Diverse Species to Uncover Innovations in Cell Type Function and Environmental Stress Resilience"
8:50 pm - 9:00 pm
Discussion
9:00 pm - 9:15 pm
"Elucidation and Targeted Engineering of Plant Nitrate-Responsive Gene Circuits"
9:15 pm - 9:20 pm
Discussion
9:20 pm - 9:30 pm
General Discussion
Tuesday
7:30 am - 8:30 am
Breakfast
8:30 am - 9:00 am
Group Photo
9:00 am - 12:30 pm
Single Cell Analysis in Stress Responses
9:00 am - 9:10 am
Introduction by Discussion Leader
9:10 am - 9:30 am
"How Aphids Remote-Control Plants: Aphid Effector Proteins in Plant Gall Induction and Agriculture"
9:30 am - 9:40 am
Discussion
9:40 am - 10:00 am
"Single-Cell and Spatially-Resolved Transcriptomics for Understanding Stress in Bioenergy Grasses"
10:00 am - 10:10 am
Discussion
10:10 am - 10:25 am
"Developing a Cell Polarity Toolkit to Direct Perturbations of Cell Division and Cell Fate to Polar Domains"
10:25 am - 10:30 am
Discussion
10:30 am - 11:00 am
Coffee Break
11:00 am - 11:20 am
"The Gene Regulatory Landscape of Flowering Plants"
11:20 am - 11:30 am
Discussion
11:30 am - 11:45 am
"Defining the Regulatory Grammar of Stress Responses in Maize"
11:45 am - 11:50 am
Discussion
11:50 am - 12:05 pm
"Single Nucleus Transcriptomics of Grapevine and Erysiphe Necator During Powdery Mildew Infection"
12:05 pm - 12:10 pm
Discussion
12:10 pm - 12:20 pm
General Discussion
12:20 pm - 12:30 pm
Poster Previews
12:30 pm - 1:30 pm
Lunch
1:30 pm - 4:00 pm
Free Time
4:00 pm - 6:00 pm
Poster Session
6:00 pm - 7:00 pm
Dinner
7:30 pm - 9:30 pm
Defining Plant Cell Types Across Plants
7:30 pm - 7:40 pm
Introduction by Discussion Leader
7:40 pm - 8:00 pm
"Auxin-Cell Wall Interplay in Moss Phyllotaxis Patterning"
8:00 pm - 8:10 pm
Discussion
8:10 pm - 8:25 pm
"An Evolutionary-Scale Single-Cell Multimodal Atlas of Leaf Development and Morphological Diversity"
8:25 pm - 8:30 pm
Discussion
8:30 pm - 8:50 pm
"Sharp Objects: Genetic and Cell Type Homology in Plant Armature Evolution"
8:50 pm - 9:00 pm
Discussion
9:00 pm - 9:15 pm
"Mechanisms of Venation Patterning in the Grass Leaf"
9:15 pm - 9:20 pm
Discussion
9:20 pm - 9:30 pm
General Discussion
Wednesday
7:30 am - 8:30 am
Breakfast
9:00 am - 12:30 pm
Spatially Resolved Single Cell Metabolomics and Proteomics
9:00 am - 9:10 am
Introduction by Discussion Leader
9:10 am - 9:30 am
"Multiplex Perturbation of Yew Reveals Cryptic Proteins and Enzymes that Enable Taxol Biosynthesis"
9:30 am - 9:40 am
Discussion
9:40 am - 9:55 am
"Analyzing the Cellular Recycling and Chloroplast Turnover by Autophagy in the Different Photosynthetic Cell Types of Maize Leaf"
9:55 am - 10:00 am
Discussion
10:00 am - 10:15 am
"Spatiotemporal Transcriptomic Mapping of Nodule Organogenesis in Medicago Reveals Cortex-Derived Cell Types Developed to Accommodate Nitrogen-Fixing Rhizobia"
10:15 am - 10:20 am
Discussion
10:20 am - 10:50 am
Coffee Break
10:50 am - 11:10 am
"The Chemistry of Stem Cells: Spatial Metabolomics in Developing Plant Tissue"
11:10 am - 11:20 am
Discussion
11:20 am - 11:35 am
"Spatially Defined Transient Cell States Regulate Differential Growth of the Apical Hook"
11:35 am - 11:40 am
Discussion
11:40 am - 11:55 am
"To Cell and Back: The Study of Plant Regeneration from Protoplasts"
11:55 am - 12:00 pm
Discussion
12:00 pm - 12:15 pm
"Spatiotemporal Regulation of Arbuscular Mycorrhizal Symbiosis at High-Resolution"
12:15 pm - 12:20 pm
Discussion
12:20 pm - 12:30 pm
General Discussion
12:30 pm - 1:30 pm
Lunch
1:30 pm - 4:00 pm
Free Time
4:00 pm - 6:00 pm
Poster Session
6:00 pm - 7:00 pm
Dinner
7:30 pm - 9:30 pm
Multiscale and Quantitative Imaging
7:30 pm - 7:40 pm
Introduction by Discussion Leader
7:40 pm - 8:00 pm
"Subcellular Defects in Plant Cell Wall Synthesis and Secretion Scale up to Whole-Plant Phenotypes"
8:00 pm - 8:10 pm
Discussion
8:10 pm - 8:30 pm
"Caught in the act: An Effector Trap Enables Visualization and Capture of Effector-Targeted Plant Cells"
8:30 pm - 8:40 pm
Discussion
8:40 pm - 8:55 pm
"Evolution of Moss Leaf-Like Organs Through Variations in Deeply Conserved Developmental Principles"
8:55 pm - 9:00 pm
Discussion
9:00 pm - 9:15 pm
"Single-Cell Genomics in Populus Stress Resilience"
9:15 pm - 9:20 pm
Discussion
9:20 pm - 9:30 pm
General Discussion
Thursday
7:30 am - 8:30 am
Breakfast
8:30 am - 9:00 am
Business Meeting
Nominations for the Next Vice Chair(s); Complete the GRC Evaluation Forms; Election of the Next Vice Chair(s)
9:00 am - 12:30 pm
Phenotyping at Cellular, Organ, and Whole Organism Levels
9:00 am - 9:10 am
Introduction by Discussion Leader
9:10 am - 9:30 am
"The Role of Mechanics in Patterning: From Gene Expression to Cell Polarity"
9:30 am - 9:40 am
Discussion
9:40 am - 9:55 am
"Single-Cell Transcriptomics Reveals the Unique and Shared Regulation of Programmed Cell Death in Xylem and Root Cap"
9:55 am - 10:00 am
Discussion
10:00 am - 10:15 am
"Understanding How Microbial Signals Stimulate Lateral Root Development at the Single Cell Level"
10:15 am - 10:20 am
Discussion
10:20 am - 10:50 am
Coffee Break
10:50 am - 11:10 am
"Dissecting Spatiotemporal Dynamics of Cell Division and Differentiation in Plant Roots"
11:10 am - 11:20 am
Discussion
11:20 am - 11:35 am
"The High-Resolution Transcriptional Landscape of Xylan Biosynthesis: Insights from Single Cell RNA-seq"
11:35 am - 11:40 am
Discussion
11:40 am - 11:55 am
"HDACs-Mediated Suppression of Remnant Identity and Stress Responses Facilitates Reprogramming"
11:55 am - 12:00 pm
Discussion
12:00 pm - 12:15 pm
"Spatiotemporal Variation of Osmotic Stress Responses in Developing Rice (Oryza Sativa) Leaves"
12:15 pm - 12:20 pm
Discussion
12:20 pm - 12:30 pm
General Discussion
12:30 pm - 1:30 pm
Lunch
1:30 pm - 4:00 pm
Free Time
4:00 pm - 6:00 pm
Poster Session
6:00 pm - 7:00 pm
Dinner
7:30 pm - 9:30 pm
Modeling and Data Integration for Single Cell Approaches
7:30 pm - 7:40 pm
Introduction by Discussion Leader
7:40 pm - 8:00 pm
"Specialised Cells for Specialised Metabolism? The Example of "Hyper Cells" and Hyperforin Biosynthetic Pathway in St John's Wort"
8:00 pm - 8:10 pm
Discussion
8:10 pm - 8:25 pm
"Benchmarking Gene-Gene Correlation Metrics and Imputation Methods in Single-Cell Transcriptomics"
8:25 pm - 8:30 pm
Discussion
8:30 pm - 8:50 pm
"Recruitment, Rewiring, and Deep Conservation in Flowering Plant Gene Regulation"
8:50 pm - 9:00 pm
Discussion
9:00 pm - 9:15 pm
"Improved Gene Prioritization in Crops Using Single-Cell Gene Regulatory Networks and Text Mining"
9:15 pm - 9:20 pm
Discussion
9:20 pm - 9:30 pm
General Discussion
Friday
7:30 am - 8:30 am
Breakfast
9:00 am
Departure