Sunday
2:00 pm - 9:00 pm
Arrival and Check-in
6:00 pm - 7:00 pm
Dinner
7:30 pm - 7:40 pm
Introductory Comments by GRC Staff / Welcome and Introduction from the Chairs
7:40 pm - 9:30 pm
Keynote Session: Expanding the Frontiers of Microscopy in Biology
7:40 pm - 8:00 pm
Introduction by Discussion Leader
8:00 pm - 8:20 pm
"CryoSeek: A Structure-First Paradigm for Discovery"
8:20 pm - 8:30 pm
Discussion
8:30 pm - 8:50 pm
"Advances in Cryogenic X-Ray Microscopy of Biological Specimens"
8:50 pm - 9:00 pm
Discussion
9:00 pm - 9:20 pm
"High-Performance Cryo-EM at the Lower End"
9:20 pm - 9:30 pm
Discussion
Monday
7:30 am - 8:30 am
Breakfast
9:00 am - 12:30 pm
Advances in Electron Detection for CryoEM
9:00 am - 9:20 am
Introduction by Discussion Leader
9:20 am - 9:40 am
"From Falcon300 to Swift100: How Will CMOS Image Sensors Help Cryo-EM to Fly Next?"
9:40 am - 9:50 am
Discussion
9:50 am - 10:10 am
"The C100 Project: Results and Outlook of Wafer Scale CMOS Sensor for 100 keV Cryo-Electron Microscopy"
10:10 am - 10:20 am
Discussion
10:20 am - 10:50 am
Coffee Break
10:50 am - 11:10 am
"A Small-Pitch Hybrid Pixel Counting Detector for CryoEM at 100 keV"
11:10 am - 11:20 am
Discussion
11:20 am - 11:40 am
"Recent Advances and Future Opportunities in Direct Detection Technology"
11:40 am - 11:50 am
Discussion
11:50 am - 12:10 pm
"Electron Imager Developments at Gatan"
12:10 pm - 12:20 pm
Discussion
12:20 pm - 12:30 pm
General Discussion
12:30 pm - 1:30 pm
Lunch
1:30 pm - 4:30 pm
Free Time
4:30 pm - 6:00 pm
Poster Session
6:00 pm - 8:00 pm
Advances in CryoEM Algorithms and Software
6:00 pm - 6:20 pm
Introduction by Discussion Leader
6:20 pm - 6:40 pm
"Automated Membrane Detection and Subtraction Aids Structure Determination of Small Membrane Proteins"
6:40 pm - 6:50 pm
Discussion
6:50 pm - 7:10 pm
"Advances in Methods for Heterogeneity Analysis"
7:10 pm - 7:20 pm
Discussion
7:20 pm - 7:30 pm
General Discussion
7:30 pm - 7:50 pm
"Model Building and Validation in Cryo-EM With Coot and Moorhen"
7:50 pm - 8:00 pm
Discussion
8:00 pm - 9:00 pm
Dinner
Tuesday
7:30 am - 8:30 am
Breakfast
8:30 am - 9:00 am
Group Photo
9:00 am - 12:30 pm
Challenging Structures: In Vitro to In Situ
9:00 am - 9:20 am
Introduction by Discussion Leader
9:20 am - 9:40 am
"Everything in Its Right Place and at High Resolution: Investigating Muscle Tissue by Cryo-ET"
9:40 am - 9:50 am
Discussion
9:50 am - 10:10 am
"High-Resolution Ab Initio Reconstruction Enables Structure Determination of Sub-30kDa Particles"
10:10 am - 10:20 am
Discussion
10:20 am - 10:50 am
Coffee Break
10:50 am - 11:10 am
"Structural Energetics of Cold Sensitivity"
11:10 am - 11:20 am
Discussion
11:20 am - 11:40 am
"Exploring Dynamics of Macromolecules by CryoEM: Ancestral Ribosomes as a Case Study"
11:40 am - 11:50 am
Discussion
11:50 am - 12:10 pm
Late-Breaking Topic
12:10 pm - 12:20 pm
Discussion
12:20 pm - 12:30 pm
General Discussion
12:30 pm - 1:30 pm
Lunch
1:30 pm - 4:30 pm
Free Time
4:30 pm - 6:00 pm
Poster Session
6:00 pm - 8:00 pm
Selected Poster Presentations
6:00 pm - 6:05 pm
Introduction by Discussion Leader
6:05 pm - 6:15 pm
Short Talk Selected from Poster Abstracts
6:15 pm - 6:20 pm
Discussion
6:20 pm - 6:30 pm
Short Talk Selected from Poster Abstracts
6:30 pm - 6:35 pm
Discussion
6:35 pm - 6:45 pm
Short Talk Selected from Poster Abstracts
6:45 pm - 6:50 pm
Discussion
6:50 pm - 7:00 pm
Short Talk Selected from Poster Abstracts
7:00 pm - 7:05 pm
Discussion
7:05 pm - 7:15 pm
Short Talk Selected from Poster Abstracts
7:15 pm - 7:20 pm
Discussion
7:20 pm - 7:30 pm
Short Talk Selected from Poster Abstracts
7:30 pm - 7:35 pm
Discussion
7:35 pm - 7:45 pm
Short Talk Selected from Poster Abstracts
7:45 pm - 7:50 pm
Discussion
7:50 pm - 8:00 pm
General Discussion
8:00 pm - 9:00 pm
Dinner
Wednesday
7:30 am - 8:30 am
Breakfast
9:00 am - 12:30 pm
Hardware Frontiers in Cryomicroscopy
9:00 am - 9:20 am
Introduction by Discussion Leader
9:20 am - 9:40 am
"Laser Phase Plate for Cyro-EM"
9:40 am - 9:50 am
Discussion
9:50 am - 10:10 am
"A New Design of the Laser Phase Plate and Prospects for Sub-Shot-Noise Electron Microscopy"
10:10 am - 10:20 am
Discussion
10:20 am - 10:50 am
Coffee Break
10:50 am - 11:10 am
"Sub-Cellular Chemical Mapping Using Correlated Cryogenic Electron and Mass Spectrometry Imaging"
11:10 am - 11:20 am
Discussion
11:20 am - 11:40 am
"Attempts to Maximize SNR in Cryo-EM: Single Electron Illumination and 4D-STEM"
11:40 am - 11:50 am
Discussion
11:50 am - 12:10 pm
"Multicolor Electron Microscopy via Cathodoluminescence"
12:10 pm - 12:20 pm
Discussion
12:20 pm - 12:30 pm
General Discussion
12:30 pm - 1:30 pm
Lunch
1:30 pm - 4:30 pm
Free Time
4:30 pm - 6:00 pm
Poster Session
6:00 pm - 8:00 pm
Advances in CryoEM Algorithms and Software
6:00 pm - 6:20 pm
Introduction by Discussion Leader
6:20 pm - 6:40 pm
"Ghostbuster With CryoSim: Creating Differentiable Simulators for Robust Structural Inference"
6:40 pm - 6:50 pm
Discussion
6:50 pm - 7:10 pm
"Membrane Protein Detection From Liposomes in Cryo-EM Images Using 2D Template Matching and Deep Learning"
7:10 pm - 7:20 pm
Discussion
7:20 pm - 7:40 pm
"Learning to Accurately Align Tilt Series With MissAlignment"
7:40 pm - 7:50 pm
Discussion
7:50 pm - 8:00 pm
General Discussion
8:00 pm - 9:00 pm
Dinner
Thursday
7:30 am - 8:30 am
Breakfast
8:30 am - 9:00 am
Business Meeting
Nominations for the Next Vice Chair(s); Complete the GRC Evaluation Forms; Election of the Next Vice Chair(s)
9:00 am - 12:30 pm
Specimen Preparation: From Cells to Microseconds
9:00 am - 9:20 am
Introduction by Discussion Leader
9:20 am - 9:40 am
"Improved Lamella Integrity and Next Generation 2DTM Expand the Visual Proteome"
9:40 am - 9:50 am
Discussion
9:50 am - 10:10 am
"Optimization of Cryosectioning Process for High-Throughput Cryo-Electron Tomography of Vitreous Sections"
10:10 am - 10:20 am
Discussion
10:20 am - 10:50 am
Coffee Break
10:50 am - 11:10 am
"Microsecond Time-Resolved CryoEM Using 3 Micron Pulsed Laser Revitrification in Liquid Ethane"
11:10 am - 11:20 am
Discussion
11:20 am - 11:40 am
"Advances in Microsecond Time-Resolved Cryo-EM"
11:40 am - 11:50 am
Discussion
11:50 am - 12:10 pm
"Back to the Future: Developing New (and Old) Approaches to Sample Preparation and Time-Resolved CryoEM"
12:10 pm - 12:20 pm
Discussion
12:20 pm - 12:30 pm
General Discussion
12:30 pm - 1:30 pm
Lunch
1:30 pm - 4:30 pm
Free Time
4:30 pm - 6:00 pm
Poster Session
6:00 pm - 8:00 pm
Selected Poster Presentations
6:00 pm - 6:05 pm
Introduction by Discussion Leader
6:05 pm - 6:15 pm
Short Talk Selected from Poster Abstracts
6:15 pm - 6:20 pm
Discussion
6:20 pm - 6:30 pm
Short Talk Selected from Poster Abstracts
6:30 pm - 6:35 pm
Discussion
6:35 pm - 6:45 pm
Short Talk Selected from Poster Abstracts
6:45 pm - 6:50 pm
Discussion
6:50 pm - 7:00 pm
Short Talk Selected from Poster Abstracts
7:00 pm - 7:05 pm
Discussion
7:05 pm - 7:15 pm
Short Talk Selected from Poster Abstracts
7:15 pm - 7:20 pm
Discussion
7:20 pm - 7:30 pm
Short Talk Selected from Poster Abstracts
7:30 pm - 7:35 pm
Discussion
7:35 pm - 7:45 pm
Short Talk Selected from Poster Abstracts
7:45 pm - 7:50 pm
Discussion
7:50 pm - 8:00 pm
General Discussion
8:00 pm - 9:00 pm
Dinner
Friday
7:30 am - 8:30 am
Breakfast
9:00 am
Departure