Sunday
2:00 pm - 9:00 pm
Arrival and Check-in
6:00 pm - 7:00 pm
Dinner
7:30 pm - 7:40 pm
Introductory Comments by GRC Staff / Welcome and Introduction from the Chairs
7:40 pm - 9:30 pm
Keynote Session: Volume Electron Microscopy of Organelles, Organs, and Organisms
7:40 pm - 7:45 pm
Introduction by Discussion Leader
7:45 pm - 8:15 pm
"Connectomics: Information vs. Understanding"
8:15 pm - 8:20 pm
Discussion
8:20 pm - 8:50 pm
"vEM and CMI: Towards Imaging From Patient to Protein"
8:50 pm - 8:55 pm
Discussion
8:55 pm - 9:25 pm
"vEM and Neuronal Connectomics in Marine Zooplankton Larvae"
9:25 pm - 9:30 pm
Discussion
Monday
7:30 am - 8:30 am
Breakfast
9:00 am - 12:30 pm
9:00 am - 9:25 am
"Using Volume Electron Microscopy in Cancer Early Detection"
9:25 am - 9:30 am
Discussion
9:30 am - 9:40 am
"Mitochondrial Architecture of Cytotrophoblast and Syncytiotrophoblast in Placenta"
9:40 am - 9:45 am
Discussion
9:45 am - 9:55 am
"Mapping Glycogen Aggregates and Identifying Ultrastructural Changes in a Lafora Disease Mouse Model"
9:55 am - 10:00 am
Discussion
10:00 am - 10:10 am
"Characterising the Blood Nerve Barrier using SEM Array Tomography"
10:10 am - 10:15 am
Discussion
10:15 am - 10:25 am
"Using vEM Techniques to Catch Nanoparticles in the Act: Toxicology and Pharmacology"
10:25 am - 10:30 am
Discussion
10:30 am - 11:00 am
Coffee Break
11:00 am - 11:25 am
"Subcellular Connectomic Analyses of Energy Networks in Striated Muscles"
11:25 am - 11:30 am
Discussion
11:30 am - 11:40 am
"Reading the Engram in the Larvae of Drosophila with Enhanced FIBSEM and Connectomics"
11:40 am - 11:45 am
Discussion
11:45 am - 11:55 am
"Cell Size Matters: Comparing Cellular Ultrastructure Between Rotifers and Paramecium"
11:55 am - 12:00 pm
Discussion
12:00 pm - 12:10 pm
"Characterizing the Ultrastructural Determinants of Collective Behavior in Choanoeca Flexa Through Volume FIB-SEM"
12:10 pm - 12:15 pm
Discussion
12:15 pm - 12:30 pm
General Discussion
12:30 pm - 1:30 pm
Lunch
1:30 pm - 4:30 pm
Free Time
3:00 pm - 4:00 pm
The GRC Power Hour™
The GRC Power Hour™ is designed to address the interests and needs of all scientists and the challenges they face in their careers. Specifically, the program promotes meaningful conversations amongst colleagues of all career stages about barriers to career advancement and strategies to support the professional growth of all members of the community. The GRC Power Hour™ is open to all conference participants.
4:30 pm - 6:00 pm
Poster Session
6:00 pm - 8:00 pm
Sample Preparation Challenges in vEM
6:00 pm - 6:25 pm
"Recent Progress in En Bloc Tissue Staining for Volume Electron Microscopy"
6:25 pm - 6:30 pm
Discussion
6:30 pm - 6:40 pm
"Carbon Black Conductive Resins for vEM: Key Features and Recent Applications"
6:40 pm - 6:45 pm
Discussion
6:45 pm - 6:55 pm
"Resin Type-Dependent Deformation Characteristics in Ultra-Thin Sections for Array Tomography"
6:55 pm - 7:00 pm
Discussion
7:00 pm - 7:25 pm
"EasyCLEM with VP CLEM KIT"
7:25 pm - 7:30 pm
Discussion
7:30 pm - 7:40 pm
"Rapid High-Pressure Freezing for Improved Brain Tissue Preservation"
7:40 pm - 7:45 pm
Discussion
7:45 pm - 7:55 pm
"Expanding Access to Cryo-EM: Evaluating Chemically Fixed Samples for Cryo-Volume Imaging"
7:55 pm - 8:00 pm
Discussion
8:00 pm - 9:00 pm
Dinner
Tuesday
7:30 am - 8:30 am
Breakfast
8:30 am - 9:00 am
Group Photo
9:00 am - 12:30 pm
High Speed, High Volume, High Fidelity vEM
9:00 am - 9:25 am
"Faster Volume Electron Microscopy with 64 Electron Beams and Optical Transmission Detection"
9:25 am - 9:30 am
Discussion
9:30 am - 9:40 am
"Microcircuits in the Marmoset Prefrontal Cortex with a Large Volume Electron Microscopy, ATUM-Blade-TEM"
9:40 am - 9:45 am
Discussion
9:45 am - 9:55 am
"vCLEM to Target Organelles in Adherent Cells"
9:55 am - 10:00 am
Discussion
10:00 am - 10:10 am
"Rewiring of Organelle Ultrastructure and Interactions in HER2-Positive Breast Cancer Revealed through Volume Electron Microscopy"
10:10 am - 10:15 am
Discussion
10:15 am - 10:25 am
"FIB-SEM and Array Tomography Workflow Enhanced by Correlative Light-Electron Microscopy for Precise Targeting of Regions of Interest in Cells and Tissue"
10:25 am - 10:30 am
Discussion
10:30 am - 11:00 am
Coffee Break
11:00 am - 11:25 am
"Array Tomography Tissue Libraries Enable the Multimodal Visualization in Neuropathology Research"
11:25 am - 11:30 am
Discussion
11:30 am - 11:40 am
"AI-Driven Adaptive Scanning provides Accelerated, Efficient and Contextual Data Acquisition for Volume Electron Microscopy using Plasma FIB-SEM"
11:40 am - 11:45 am
Discussion
11:45 am - 11:55 am
"3D Targeting in Cryo-Volume Imaging of High-Pressure Frozen Samples"
11:55 am - 12:00 pm
Discussion
12:00 pm - 12:10 pm
"FAST-ET: Fast Automated Serial Tape-Enabled Electron Tomography"
12:10 pm - 12:15 pm
Discussion
12:15 pm - 12:30 pm
General Discussion
12:30 pm - 1:30 pm
Lunch
1:30 pm - 4:30 pm
Free Time
4:30 pm - 6:00 pm
Poster Session
6:00 pm - 8:00 pm
Analysis and Quantitation in vEM
6:00 pm - 6:25 pm
"Multimodal Lung Cell Atlas at the Nanometre Scale"
6:25 pm - 6:30 pm
Discussion
6:30 pm - 6:40 pm
"From Connectomics to Cellomics: Reconstructing a Whole Animal Using Deep Learning Methods"
6:40 pm - 6:45 pm
Discussion
6:45 pm - 6:55 pm
"Volume EM by FIB-SEM Enables Quantitative 3D Characterization of Coxiella Burnetii Infection"
6:55 pm - 7:00 pm
Discussion
7:00 pm - 7:25 pm
"Deciphering the Subcelular Architecture of Metabolic Tissues using FIB-SEM"
7:25 pm - 7:30 pm
Discussion
7:30 pm - 7:40 pm
"High-Throughput Quantification of Membrane Contact Sites on Large-Scale Electron Microscopy Imaging"
7:40 pm - 7:45 pm
Discussion
7:45 pm - 7:55 pm
"From Volume Electron Microscopy Datasets to Model Segmentation and Feature Quantification"
7:55 pm - 8:00 pm
Discussion
8:00 pm - 9:00 pm
Dinner
Wednesday
7:30 am - 8:30 am
Breakfast
9:00 am - 12:30 pm
Tools to Accelerate, Smooth and Widen the vEM Pipeline
9:00 am - 9:25 am
"Foundation Models for Electron Microscopy Image Analysis"
9:25 am - 9:30 am
Discussion
9:30 am - 9:40 am
"A Lightweight Pipeline for Classification of 3D Textural Patterns and Their Annotation"
9:40 am - 9:45 am
Discussion
9:45 am - 9:55 am
"SynapseNet: Deep Learning for Automatic Synapse Reconstruction"
9:55 am - 10:00 am
Discussion
10:00 am - 10:10 am
"Development of a Pipeline to Characterize Hepatic Organelles in Disease Models"
10:10 am - 10:15 am
Discussion
10:15 am - 10:45 am
Coffee Break
10:45 am - 11:10 am
"Probing the Structure and Function of the Healthy and Diseased Brain with X-Rays, Electrons and Light"
11:10 am - 11:15 am
Discussion
11:15 am - 11:25 am
"Concept for Routine Generation of N >> 1 Medium-Scale Ultrastructure Volumes"
11:25 am - 11:30 am
Discussion
11:30 am - 11:40 am
"Automating SBF-SEM Imaging Based on X-Ray Maps"
11:40 am - 11:45 am
Discussion
11:45 am - 11:55 am
"Enabling High-Resolution Cryo-FIB-SEM Volume Imaging through Specialized Serial Acquisition"
11:55 am - 12:00 pm
Discussion
12:00 pm - 12:10 pm
"Advanced Scan Control for Dose Efficient Imaging to Accelerate Cryo-Volume Electron Microscopy"
12:10 pm - 12:15 pm
Discussion
12:15 pm - 12:30 pm
General Discussion
12:30 pm - 1:30 pm
Lunch
1:30 pm - 4:30 pm
Free Time
4:30 pm - 6:00 pm
Poster Session
6:00 pm - 8:00 pm
Visualizing and Sharing Big vEM Data
6:00 pm - 6:25 pm
"Understanding Cellular Architecture with Interactive Machine Learning"
6:25 pm - 6:30 pm
Discussion
6:30 pm - 6:40 pm
"Automated Detection of Nuclear Pores in 3D Electron Microscopy Data of Mammalian Cortex"
6:40 pm - 6:45 pm
Discussion
6:45 pm - 7:10 pm
"Correlative Big Image Data Sharing, Registration and Exploration"
7:10 pm - 7:15 pm
Discussion
7:15 pm - 7:25 pm
"Cavalieri Principle for Fast and Straightforward Volume EM Data Analysis"
7:25 pm - 7:30 pm
Discussion
7:30 pm - 7:40 pm
"CebraEM: A Workflow for Efficient 3D Organelle Segmentation in Volume SEM"
7:40 pm - 7:45 pm
Discussion
7:45 pm - 7:55 pm
7:55 pm - 8:00 pm
Discussion
8:00 pm - 9:00 pm
Dinner
Thursday
7:30 am - 8:30 am
Breakfast
8:30 am - 9:00 am
Business Meeting
Nominations for the Next Vice Chair(s); Complete the GRC Evaluation Forms; Election of the Next Vice Chair(s)
9:00 am - 12:30 pm
Combining and Correlating vEM with Other Technologies
9:00 am - 9:25 am
"FIB-SEM of Biomineralized Tissues"
9:25 am - 9:30 am
Discussion
9:30 am - 9:40 am
"Megakaryocytes use Podosomes to Penetrate the Endothelial Barrier of Bone Marrow Sinusoids"
9:40 am - 9:45 am
Discussion
9:45 am - 9:55 am
"Integration of 3D Elemental Mapping with SBF-SEM"
9:55 am - 10:00 am
Discussion
10:00 am - 10:10 am
"Illuminating Protein Aggregate Clearance by Correlative Light and Electron Microscopy in Tokuyasu Cryo-Sections"
10:10 am - 10:15 am
Discussion
10:15 am - 10:25 am
"Deciphering Dinomitosis: A vCLEM Approach"
10:25 am - 10:30 am
Discussion
10:30 am - 11:00 am
Coffee Break
11:00 am - 11:25 am
"Correlative CryoFIB/SEM Volume Imaging of Native Cells"
11:25 am - 11:30 am
Discussion
11:30 am - 11:40 am
"Excavating Growth Cone Architecture from the Developing Neocortex with VolumeEM"
11:40 am - 11:45 am
Discussion
11:45 am - 11:55 am
"3D Chromatin Architecture By Volume EM"
11:55 am - 12:00 pm
Discussion
12:00 pm - 12:10 pm
"Automated Registration for Joint Analysis of 3D smFISH Gene Expression Data and Electron Microscopy"
12:10 pm - 12:15 pm
Discussion
12:15 pm - 12:30 pm
General Discussion
12:30 pm - 1:30 pm
Lunch
1:30 pm - 4:30 pm
Free Time
4:30 pm - 6:00 pm
Poster Session
6:00 pm - 8:00 pm
Nascent Technological Forays in vEM
6:00 pm - 6:25 pm
"Industrial Scale Brain Mapping"
6:25 pm - 6:30 pm
Discussion
6:30 pm - 6:40 pm
"EMcapsulins: An Expanded Volume EM Reporter Platform for Correlative Cell and Brain Imaging"
6:40 pm - 6:45 pm
Discussion
6:45 pm - 6:55 pm
"Investigation of Signal Generation in Optical STEM for Multibeam Applications"
6:55 pm - 7:00 pm
Discussion
7:00 pm - 7:25 pm
"Postmitotic Cilia Deconstruction in the Cerebellum: A New Model of Cilia Disassembly"
7:25 pm - 7:30 pm
Discussion
7:30 pm - 7:40 pm
"Combining Synchrotron-Based microCT with Plasma FIB-SEM for Targeted Volume EM"
7:40 pm - 7:45 pm
Discussion
7:45 pm - 7:55 pm
"Unveiling the 3D Architecture of Human Retinal Cone and Rod Cells Using Cryo-Volume Electron Microscopy"
7:55 pm - 8:00 pm
Discussion
8:00 pm - 9:00 pm
Dinner
Friday
7:30 am - 8:30 am
Breakfast
9:00 am
Departure